Engineering Candida tenuis xylose reductase for improved utilization of NADH: Antagonistic effects of multiple side chain replacements and performance of site-directed mutants under simulated in vivo conditions

Applied and Environmental Microbiology

Volumn 71, Issue 10, 2005, Pages 6390-6393

  Petschacher, Barbara ^a   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME KINETICS; MICROBIOLOGY; MOLECULAR STRUCTURE; MUTAGENESIS;

CHAIN REPLACEMENTS; KINETIC MECHANISM; PHYSIOLOGICAL REACTANTS; SUBSTITUTION;

ENZYMES;

ARGININE; BACTERIAL ENZYME; HISTIDINE; LYSINE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; XYLOSE REDUCTASE;

METABOLISM;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CANDIDA; CANDIDA TENUIS; CHEMICAL REACTION KINETICS; COMPUTER PREDICTION; CONCENTRATION RESPONSE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME STRUCTURE; IN VIVO STUDY; NONHUMAN; PHOSPHATE TRANSPORT; PROTEIN ENGINEERING; SIMULATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; WILD TYPE;

ALDEHYDE REDUCTASE; CANDIDA; COENZYMES; GENETIC ENGINEERING; KINETICS; MUTAGENESIS, SITE-DIRECTED; NAD; SUBSTRATE SPECIFICITY; XYLOSE;

CANDIDA TENUIS;

EID: 26844452043     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.10.6390-6393.2005     Document Type: Article

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