Disruption of oligomerization induces nucleocytoplasmic shuttling of leukemia-associated Rho guanine-nucleotide exchange factor

Molecular Pharmacology

Volumn 72, Issue 4, 2007, Pages 993-1002

  Grabocka, Elda ^a   Wedegaertner, Philip B ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; ISOLEUCINE; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DIMERIZATION; GENE DISRUPTION; LEUKEMIA; NONHUMAN; NUCLEOCYTOPLASMIC TRANSPORT; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BIOPOLYMERS; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; COS CELLS; CYTOPLASM; DNA PRIMERS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; MUTAGENESIS, SITE-DIRECTED; NUCLEAR LOCALIZATION SIGNALS; PROTEIN CONFORMATION; PROTEIN TRANSPORT;

EID: 34748839538     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.107.035162     Document Type: Article

References (47)

1. Balboa MA and Insel P (1995) Nuclear phospholipase D in Madin-Darby canine kidney cells. Guanosine 5′-O-(thiotriphosphate)-stimulated activation is mediated by RhoA and is downstream of protein kinase C. J Biol Chem 270:29843-29847.
2. Baldassare JJ, Jarpe MB, Alferes L, and Raben D (1997) Nuclear translocation of RhoA mediates the mitogen induced activation of phospholipase D involved in nuclear envelope signal transduction. J Biol Chem 272:4911-4914.
3. Banaszynski LA, Liu CW, and Wandless TJ (2005) Characterization of the FKBP.rapamycin. FRB ternary complex. J Am Chem Soc 127:4715-4721.
4. Banerjee J and Wedegaertner PB (2004) Identification of a novel sequence in PDZ-RhoGEF that mediates interaction with the actin cytoskeleton. Mol Biol Cell 15:1760-1775.
5. Basile JR, Barac A, Zhu T, Guan KL, and Gutkind JS (2004) Class IV semaphorins promote angiogenesis by stimulating Rho-initiated pathways through plexin-B. Cancer Res 64:5212-5224.
6. Bayle JH, Grimley JS, Stankunas K, Gestwicki JE, Wandless TJ, and Crabtree GR (2006) Rapamycin analogs with differential binding specificity permit orthogonal control of protein activity. Chem Biol 13:99-107.
7. Bhattacharyya R and Wedegaertner PB (2000) Gα 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115RhoGEF membrane binding. J Biol Chem 275:14992-14999.
8. Bhattacharyya R and Wedegaertner PB (2003) Characterization of Galpha13-dependent plasma membrane recruitment of p115RhoGEF. Biochem J 371:709-720.
9. Burchett SA (2003) In through the out door: nuclear localization of the regulators of G-protein signaling. J Neurochem 87:551-559.
10. Chikumi H, Barac A, Behbahani B, Gao Y, Teramoto H, Zheng Y, and Gutkind JS (2004) Homo- and hetero-oligomerization of PDZ-RhoGEF, LARG and p115RhoGEF by their C-terminal region regulates their in vivo Rho GEF activity and transforming potential. Oncogene 23:233-240.
11. Dhanasekaran N and Prasad MV (1998) G protein subunits and cell proliferation. Biol Signals Recept 7:109-117.
12. Eisenhaure TM, Francis SA, Willison LD, Coughlin SR, and Lerner DJ (2003) The Rho guanine nucleotide exchange factor LSC homo-oligomerizes and is negatively regulated through domains in its carboxyl terminus that are absent in novel splenic isoforms. J Biol Chem 278:30975-30984.
13. Fromm C, Coso OA, Montaner S, Xu N, and Gutkind JS (1997) The small GTP-binding protein Rho links G protein-coupled receptors and Gα12 to the serum response element and to cellular transformation. Proc Natl Acad Sci U S A 94:10098-10103.
14. Fukuhara S, Chikumi H, and Gutkind JS (2000) Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G12 family to Rho. FEBS Lett 485:183-188.
15. Fukuhara S, Chikumi H, and Gutkind JS (2001) RGS-containing RhoGEFs: the missing link between transforming G proteins and Rho? Oncogene 20:1661-1668.
16. Fukuhara S, Murga C, Zohar M, Igishi T, and Gutkind JS (1999) A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho. J Biol Chem 274:5868-5879.
17. Grabocka E and Wedegaertner PB (2005) Functional consequences of G alpha 13 mutations that disrupt interaction with p115RhoGEF. Oncogene 24:2155-2165.
18. Hart MJ, Jiang X, Kozasa T, Roscoe W, Singer WD, Gilman AG, Sternweis PC, and Bollag G (1998) Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13. Science 280:2112-2114.
19. Jans DA, Xiao C-Y, and Lam MHC (2000) Nuclear targeting signal recognition: a key point in nuclear transport? Bioessays 22:532-544.
20. Kourlas PJ, Strout MP, Becknell B, Veronese ML, Croce CM, Theil KS, Krahe R, Ruutu T, Knuutila S, Bloomfield CD, et al. (2000) Identification of a gene at 11q23 encoding a guanine nucleotide exchange factor: evidence for its fusion with MLL in acute myeloid leukemia. Proc Natl Acad Sci U S A 97:2145-2150.
21. Kozasa T, Jiang X, Hart MJ, Sternweis PM, Singer WD, Gilman AG, Bollag G, and Sternweis PC (1998) p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Science 280:2109-2111.
22. Kutay U and Guttinger S (2005) Leucine-rich nuclear-export signals: born to be weak. Trends Cell Biol 15:121-124.
23. Li ZY, Liu DP, and Liang CC (2005) New Insight into the molecular mechanisms of MLL-associated leukemia. Leukemia 19:183-190.
24. Lupas A (1997) Predicting coiled-coil regions in proteins. Curr Opin Struct Biol 7:388-393.
25. Lupas A, Van Dyke M, and Stock J (1991) Predicting coiled coils from protein sequences. Science 252:1162-1164.
26. Muthuswamy SK, Gilman M, and Brugge JS (1999) Controlled dimerization of ErbB receptors provides evidence for differential signaling by homo- and heterodimers. Mol Cell Biol 19:6845-6857.
27. Nakai K and Horton P (1999) PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci 24:34-36.
28. Paulmurugan R and Gambhir SS (2005) Novel fusion protein approach for efficient high-throughput screening of small molecule-mediating protein-protein interactions in cells and living animals. Cancer Res 65:7413-7420.
29. Popovic R and Zeleznik-Le N (2005) MLL-how complex does it get. J Cell Biochem 95:234-242.
30. Rossman KL, Der CJ, and Sondek J (2005) GEF means go: turning on RhoGTPases with guanine nucleotide exchange factors. Nat Rev Mol Cell Biol 6:167-180.
31. Sah VP, Seasholtz TM, Sagi SA, and Brown JH (2000) The role of Rho in G protein-coupled receptor signal transduction. Annu Rev Pharmacol Toxicol 40:459-489.
32. Sahai E and Marshall CJ (2002) Rho-GTPases and cancer. Nature Rev Cancer 2:133-142.
33. Schmidt A and Hall A (2002a) Guanine nucleotide exchange factors for Rho GTPases: turning on the switch. Genes Dev 16:1587-1609.
34. Schmidt A and Hall A (2002b) The rho exchange factor Net1 is regulated by nuclear sequestration. J Biol Chem 277:14581-14588.
35. Seasholtz TM, Majumdar M, and Brown JH (1999) Rho as a mediator of G protein-coupled receptor signaling. Mol Pharmacol 55:949-956.
36. Shaner NC, Steinbach PA, and Tsien RY (2005) A guide to choosing fluorescent proteins. Nat Methods 2:905-909.
37. Shih LY, Liang DC, Fu JF, Wu JH, Wang PN, Lin TL, Dunn P, Kuo MC, Tang TC, Lin TH, et al. (2006) Characterization of fusion partner genes in 114 patients with de novo acute myeloid leukemia and MLL rearrangement. Leukemia 20:218-223.
38. Stommel JM, Marchenko ND, Jimenez GS, Moll UM, Hope TJ, and Wahl GM (1999) A leucine-rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking. EMBO J 18:1660-1672.
39. Suzuki N, Nakamura S, Mano H, and Kozasa T (2003) Gα12 activates Rho GTPase through tyrosine-phosphorylated leukemia-associated RhoGEF. Proc Natl Acad Sci U S A 100:733-738.
40. Tanaka T, Nishimura D, Wu RC, Amano M, Iso T, Kedes L, Nishida H, Kaibuchi K, and Hamamori Y (2006) Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase. J Biol Chem 281:15320-15329.
41. Tatsumoto T, Xie X, Blumenthal R, Okamoto I, and Miki T (1999) Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. J Cell Biol 147:921-928.
42. Taya S, Inagaki N, Sengiku H, Makino H, Iwamatsu A, Urakawa I, Nagao K, Kataoka S, and Kaibuchi K (2001) Direct interaction of insulin-like growth factor-1 receptor with leukemia-associated RhoGEF. J Cell Biol 155:809-820.
43. Tyybakinoja A, Saarinen-Pihkala U, Elonen E, and Knuutila S (2006) Amplified, lost, and fused genes in 11q23-25 amplicon in acute myeloid leukemia, an array-CGH study. Genes Chromosomes Cancer 45:257-264.
44. Wang P, Wu Y, Ge X, Ma L, and Pei G (2003) Subcellular localization of β-arrestins is determined by their intact N domain and the nuclear export signal at the C terminus. J Biol Chem 278:11648-11653.
45. Wang Y and Elion EA (2003) Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell 14:2543-2558.
46. Watanabe M, Masuyama N, Fukuda M, and Nishida E (2000) Regulation of intracellular dynamics of Smad4 by its leucine-rich nuclear export signal. EMBO Rep 1:176-182.
47. Yamada T, Ohoka Y, Kogo M, and Inagaki S (2005) Physical and functional interactions of the lysophosphatidic acid receptors with PDZ domain-containing Rho guanine nucleotide exchange factors (RhoGEFs). J Biol Chem 280:19358-19363.