Novel fusion protein approach for efficient high-throughput screening of small molecule-mediating protein-protein interactions in cells and living animals

Cancer Research

Volumn 65, Issue 16, 2005, Pages 7413-7420

  Paulmurugan, Ramasamy ^a   Gambhir, Sanjiv S ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; FK 506 BINDING PROTEIN; FRB PROTEIN; HYBRID PROTEIN; LUCIFERASE; PROTEIN; RAPAMYCIN; TACROLIMUS; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DIMERIZATION; FLOW CYTOMETRY; GENETIC COMPLEMENTATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; VALIDATION PROCESS; XENOGRAFT;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; GREEN FLUORESCENT PROTEINS; HUMANS; LUCIFERASES, RENILLA; MICE; MICE, NUDE; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT FUSION PROTEINS; SIROLIMUS; TACROLIMUS BINDING PROTEINS; TRANSFECTION;

EID: 23844476035     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: 10.1158/0008-5472.CAN-05-0588     Document Type: Article

References (22)

1. Fields S, Song O. A novel genetic system to detect protein-protein interactions. Nature 1989;340:245-6.
2. Johnsson N, Varshavsky A. Split ubiquitin as a sensor of protein interactions in vivo. Proc Natl Acad Sci USA 1994;91:10340-4.
3. Rossi F, Charlton CA, Blau HM. Monitoring protein-protein interactions in intact eukaryotic cells by β-galactosidase complementation. Proc Natl Acad Sci USA 1997;94:8405-10.
4. Sato M, Ozawa T, Inukai K, Asano T, Umezawa Y. Fluorescent indicators for imaging protein phosphorylation in single living cells. Nat Biotechnol 2002;20:287-94.
5. Pollok BA, Heim R. Using GFP in FRET-based applications. Trends Cell Biol 1999;9:57-60.
6. Paulmurugan R, Ray P, De A, Chan CT, Gambhir SS. Imaging protein-protein interactions in living subjects. In: Erica Golemis, editor. 2nd ed. Protein-protein interactions: a molecular cloning manual. New York: CSHL Press; 2005. In press.
7. Paulmurugan R, Ray P, De A, Chan CT, Gambhir SS. Imaging protein-protein interactions in living subjects. TrAC Trends in Analytical Chemistry 2005;24:446-58.
8. Pelletier JN, Arndt KM, Pluckthun A, Michnick SW. An in vivo library-versus-library selection of optimized protein-protein interactions. Nat Biotechnol 1999;17:683-90.
9. Pelletier JN, Campbell-Valois FX, Michnick SW. Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments. Proc Natl Acad Sci U S A 1998;95:12141-6.
10. Wehrman T, Kleaveland B, Her JH, Balint RF, Blau HM. Protein-protein interactions monitored in mammalian cells via complementation of β-lactamase enzyme fragments. Proc Natl Acad Sci USA 2002;99:3469-74.
11. Paulmurugan R, Gambhir SS. Monitoring protein-protein interactions using split synthetic Renilla luciferase protein-fragment-assisted complementation. Anal Chem 2003;75:1584-9.
12. Ghosh I, Hamilton AD, Regan L. Antiparallel leucine-zipper directed protein reassembly: application to the green fluorescent protein. J Am Chem Soc 2000;122:5658-9.
13. Paulmurugan R, Massoud TF, Huang J, Gambhir SS. Molecular imaging of drug-modulated protein-protein interactions in living subjects. Cancer Res 2004;64:2113-9.
14. Paulmurugan R, Umezawa Y, Gambhir SS. Noninvasive imaging of protein-protein interactions in living subjects using reporter protein complementation and reconstitution strategies. Proc Natl Acad Sci U S A 2002; 99:15608-13.
15. Luker KE, Piwnica-Worms D. Optimizing luciferase protein fragment complementation for bioluminescent imaging of protein-protein interactions in live cells and animals. Methods Enzymol 2004;385:349-60.
16. Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, Piwnica-Worms D. Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals. Proc Natl Acad Sci U S A 2004;101:12288-93.
17. Bhaumik S, Gambhir SS. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proc Natl Acad Sci U S A 2002;99:377-82.
18. Massoud TF, Gambhir SS. Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev 2003;17:545-80.
19. Rossi FM, Blakely BT, Blau HM. Interaction blues: protein interactions monitored in live mammalian cells by β-galactosidase complementation. Trends Cell Biol 2000;10:119-22.
20. Ray P, Pimenta H, Paulmurugan R, et al. Noninvasive quantitative imaging of protein-protein interactions in living subjects. Proc Natl Acad Sci U S A 2002;99:3105-10.
21. Massoud TF, Paulmurugan R, Gambhir SS. Molecular imaging of homodimeric protein-protein interactions in living subjects. FASEB J 2004;18:1105-7.
22. Ozawa T, Kaihara A, Sato M, Tachihara K, Umezawa Y. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing. Anal Chem 2001;73:2516-21.