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This paper describes the preliminary results from the AGRIKOLA project, the largest, most systematic application of RNAi in plants to date. It contains detailed protocols for high-throughput cloning of RNAi constructs.
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A superb demonstration of the potential of RNAi in plant biotechnology. Gossypol toxin, normally found throughout the plant, improves pest tolerance of cotton but makes the oil extracted from seeds inedible. By specifically reducing expression of δ-cadinene synthase in developing seeds using tissue-specific RNAi, the authors succeeded in producing gossypol-free seeds whilst maintaining normal levels of the toxin elsewhere in the plant. If this approach turns out to be stable in the field and passes regulatory hurdles, it could considerably increase the value of cottonseed oil.
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Sunilkumar, G.1
Campbell, L.M.2
Puckhaber, L.3
Stipanovic, R.D.4
Rathore, K.S.5
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