Constructs and methods for hairpin RNA-mediated gene silencing in plants

Methods in Enzymology

Volumn 392, Issue , 2005, Pages 24-35

  Helliwell, Chris A ^a   Waterhouse, Peter M ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA;

ARTICLE; CONTROLLED STUDY; GENE CONSTRUCT; GENE EXPRESSION; GENE SEQUENCE; GENE SILENCING; GENE TARGETING; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; RNA DEGRADATION;

BASE SEQUENCE; DNA PRIMERS; GENE SILENCING; GENETIC VECTORS; NUCLEIC ACID CONFORMATION; PLANTS; RNA, PLANT;

EUKARYOTA;

EID: 11844286931     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(04)92002-2     Document Type: Article

References (12)

1. Fraser A.G., Kamath R.S., Zipperlen P., Martinez-Campos M., Sohrmann M., Ahringer J. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature. 408:2000;325-330
2. Gleave A.P. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol. Biol. 20:1992;1203-1207
3. Hanahan D. Techniques for transformation of E. coli in DNA cloning. Glover D. "DNA Cloning: A Practical Approach" Vol. 1:1985;109-138 IRL Press, London
4. Helliwell C.A., Wesley S.V., Wielopolska A.J., Waterhouse P.M. High throughput vectors for efficient gene silencing in plants. Func. Plant Biol. 29:2002;1217-1225
5. Hilson P., Small I., Kuiper M.T. European consortia building integrated resources for Arabidopsis functional genomics. Curr. Opin. Plant Biol. 6:2003;426-429
6. Kamath R.S., Fraser A.G., Dong Y., Poulin G., Durbin R., Gotta M., Kanapin A., Le Bot N., Moreno S., Sohrmann M., Welchman D.P., Zipperlen P., Ahringer J. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature. 421:2003;231-237
7. Sharp P.A. RNA interference - 2001. Genes Dev. 15:2001;485-490
8. Smith N.A., Singh S.P., Wang M.B., Stoutjesdijk P.A., Green A.G., Waterhouse P.M. Total silencing by intron-spliced hairpin. Nature. 407:2000;319-320
9. Wang M.B., Upadhyaya N.M., Brettell R.I.S., Waterhouse P.M. Intron mediated improvement of a selectable marker gene for plant transformation using Agrobacterium tumefaciens. J. Genet. Breed. 51:1997;325-334
10. Waterhouse P., Griffiths A.D., Johnson K.S., Winter G. Combinatorial infection and in vivo recombination: A strategy for making large phage antibody repertoires. Nucleic Acids Res. 21:1993;2265-2266
11. Waterhouse P.M., Wang M.B., Lough T. Gene silencing as an adaptive defence against viruses. Nature. 411:2001;834-842
12. Wesley S.V., Helliwell C.A., Smith N.A., Wang M.B., Rouse D.T., Liu Q., Gooding P.S., Singh S.P., Abbott D., Stoutjesdijk P.A., Robinson S.P., Gleave A.P., Waterhouse P.M. Constructs for efficient, effective and high throughput gene silencing in plants. Plant J. 27:2001;581-590