Temporal and spatial control of gene silencing in transgenic plants by inducible expression of double-stranded RNA

Plant Journal

Volumn 36, Issue 5, 2003, Pages 731-740

  Chen, Shuai ^a   Hofius, Daniel ^a   Sonnewald, Uwe ^a   Börnke, Frederik ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

Author keywords

Chemically inducible expression; Double stranded RNA; Ethanol; Functional genomics; Gene silencing; Nicotiana tabacum

Indexed keywords

BIOSYNTHESIS; CHLOROPHYLL; DEGRADATION; ENZYMES; ETHANOL; GENES; RNA;

GENE SILENCING;

PLANTS (BOTANY);

ENZYMES; ETHANOL; GENE SILENCING; GENETIC TECHNIQUES; KINETICS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; RNA, DOUBLE-STRANDED; TOBACCO; TRANSCRIPTION, GENETIC;

EMBRYOPHYTA; NICOTIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0344237357     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2003.01914.x     Document Type: Article

References (58)

1. Baulcombe, D.C. (1999) Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant Biol. 2, 109-113.
2. Baulcombe, D. (2002) RNA silencing. Curr. Biol. 12, R82-R84.
3. Béclin, C., Beutet, S., Waterhouse, P. and Vaucheret, H. (2002) A branched pathway for transgene-induced RNA silencing in plants. Curr. Biol. 12, 684-688.
4. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucl. Acids Res. 12, 8711-8721.
5. Boutla, A., Kalantidis, K., Tavernarakis, N., Tsagris, M. and Tabler, M. (2002) Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing. Nucl. Acid Res. 30, 1688-1694.
6. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
7. Caddick, M.X., Greenland, A.J., Jepson, I., Krause, K.P., Qu, N., Riddell, K.V., Salter, M.G., Schuch, W., Sonnewald, U. and Tomsett, A.B. (1998) An ethanol inducible gene switch for plants used to manipulate carbon metabolism. Nat. Biotechnol. 16, 177-180.
8. Carthew, R.W. (2001) Gene silencing by double stranded RNA. Curr. Opin. Cell Biol. 13, 244-248.
9. Chuang, C.H. and Meyerowitz, E.M. (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA, 97, 4985-4990.
10. Cogoni, C. and Macino, G. (2000) Post-transcriptional gene silencing across kingdoms. Curr. Opin. Genet. Dev. 10, 638-643.
11. Dalmay, T., Hamilton, A., Mueller, E. and Baulcombe, D. (2000) An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell, 101, 543-553.
12. Frommer, W.B. and Sonnewald, U. (1995) Molecular analysis of carbon partitioning in solanaceous species. J. Exp. Bot. 46, 587-607.
13. Goff, S.A., Ricke, D., Lan, T.H. et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science, 296, 92-100.
14. Gosselé, V., Faché, I., Meulewataeter, F., Cornelissen, M. and Metzlaff, M. (2002) SVISS - a novel transient gene silencing system for gene function discovery and validation in tobacco plants. Plant J. 32, 859-866.
15. Guo, H.S., Fei, J.F., Xie, Q. and Chua, N.H. (2003) A chemical-regulated inducible RNAi system in plants. Plant J. 34, 383-392.
16. Hamilton, A.J. and Baulcombe, D. (1999) A species of small antisense RNA in posttranscriptional gene silencing in plants. Science, 286, 950-952.
17. Hammond, S.M., Bernstein, E., Beach, D. and Hannon, G.J. (2000) An RNA-directed nuclease mediates post-transcriptiohal gene silencing in Drosophila cells. Nature, 404, 293-296.
18. Härtel, H., Kruse, E. and Grimm, B. (1997) Restriction of chlorophyll synthesis due to expression of glutamate 1-semialdehyde aminotransferase antisense RNA does not reduce the light-harvesting antenna size in tobacco. Plant Physiol. 113, 1113-1124.
19. He, Z.-H., Li, J., Sundqvist C. and Timko, M.P. (1994) Leaf developmental age controls expression of genes encoding enzymes of chlorophyll and heme biosynthesis in pea (Pisum sativum L.). Plant Physiol. 106, 537-546.
20. Hiriart J.-B., Lehto, K., Tyystjärvi, E., Junttila, T. and Aro, E.-M. (2002) Suppression of a key gene involved in chlorophyll biosynthesis by means of virus induced gene silencing. Plant. Mol. Biol. 50, 213-224.
21. Höfgen, R., Axelsen, K.A., Kannangara, C.G., Schüttke, I., Pohlenz, H.-D., Willmitzer, L., Grimm, B. and von Wettstein, D. (1994) A visible marker for antisense mRNA expression in plants: inhibition of chlorophyll synthesis with a glutamate 1-semialdehyde aminotransferase antisense gene. Proc. Natl. Acad. Sci. USA, 91, 1726-1730.
22. Holzberg, S., Brosio, P., Gross, C. and Pogue, G.P. (2002) Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J. 30, 315-327.
23. Junker, B.H., Chu, C., Sonnewald, U., Willmitzer, L. and Fernie, A.R. (2003) In plants the ale gene expression system responds more rapidly following induction with acetaldehyde than with ethanol. FEBS Lett. 535, 136-140.
24. Klahre, U., Crété, P., Leuenberger, S.A., Iglesias, V.A. and Meins, F. (2002) High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants. Proc. Natl. Acad. Sci. USA, 99, 11981-11986.
25. van der Krol, A., Lenting, P.E., Veenstra, J., van der Meer, I.M., Koes, R.E., Gerats, A.G.M., Mol, J.N.M. and Stuitje, A.R. (1988) An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature, 333, 866-869.
26. van der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N. and Stuitje, A.R. (1990) Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell, 2, 291-299.
27. Levin, J.Z., de Framond, A.J., Tuttle, A., Bauer, M.W. and Heifetz, P.B. (2000) Methods of double-stranded RNA-mediated gene inactivation in Arabidopsis and their use to define an essential gene methionine biosynthesis. Plant Mol. Biol. 44, 759-775.
28. Lichtenthaler, H.K. (1987) Chlorophylls and caroteinoides: pigments of photosynthetic biomembranes. Meth. Enzymol. 148, 350-382.
29. Liu, Y., Schiff, M. and Dinesh-Kumar, S.P. (2002) Virus-induced gene silencing in tomato. Plant J. 31, 777-786.
30. Logemann, J., Schell, J. and Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163, 16-20.
31. Matzke, M., Matzke, A.J. and Kooter, J.M. (2001) RNA: guiding gene silencing. Science, 293, 1080-1083.
32. Mishra, K.K. and Hanada, A.K. (1998) Post-transcriptional silencing of pectin methylesterase gene in transgenic tomatos fruits results from impaired pre-mRNA processing. Plant J. 14, 583-592.
33. Mlotshwa, S., Voinnet, O. Mette, M.F., Matzke, M., Vaucheret, H., Ding, S.W., Pruss, G. and Vance, V.B. (2002) RNA silencing and the mobile silencing signal. Plant Cell, 14, S289-S301.
34. Mourrain, P., Béclin, C., Elmayan, T. et al. (2000) Arabidopsis SGS2 and SGS3 are required for posttranscriptional gene silencing and natural virus resistance. Cell, 101, 533-542.
35. Napoli, C., Lemieux, C. and Jorgensen, R. (1990) Introduction of chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell, 2, 279-289.
36. Page, D.R. and Grossniklaus, U. (2002) The art and design of genetic screens: Arabidopsis thaliana. Nat. Rev. Genet. 3, 124-136.
37. Palauqui, J.C. and Vaucheret H. (1998) Transgenes are dispensable for the RNA degradation step of cosuppression. Proc. Natl Acad. Sci. USA, 95, 9675-9680.
38. Palauqui, J.C., Elmayan, T., Pollien, J.M. and Vaucheret, H. (1997) Systemic acquired silencing: transgene-specific posttranscriptional gene silencing is transmitted by grafting from stocks to non-silenced scions. EMBO J. 16, 4738-4745.
39. Papenbrock, J., Pfündel, E., Mock, H.-P. and Grimm, B. (2000) Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants. Plant J. 22, 155-164.
40. Plasterk, R.H. (2002) RNA silencing: the genome's immune system. Science, 296, 1263-1265.
41. Ratcliff, F., Martin-Hernandez, A.M. and Baulcombe, D.C. (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25, 237-245.
42. Rosahl, S., Schell, J. and Willmitzer, L. (1987) Expression of a tuber-specific storage protein in transgenic tobacco plants: demonstration of an esterase activity. EMBO J. 6, 1155-1159.
43. Roslan, H.A., Salter, M.G., Wood, C.D. et al. (2001) Characterisation of the ethanol-inducible ale gene expression system in Arabidopsis thaliana. Plant J. 28, 225-235.
44. Salter, M.G., Paine, J.A., Riddel, K.V., Jepson, I., Greenland, A.J., Caddick, M.X. and Tomsett, B.A. (1998) Characterisation of the ethanol-inducible ale gene expression system for transgenic plants. Plant J. 16, 127-132.
45. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
46. Smith, N.A., Singh, S.P., Wang, N.B., Stoutjesdijk, P.A., Green, A.G. and Waterhouse, P.M. (2000) Total silencing by intron-spliced hairpin RNAs. Nature, 407, 319-320.
47. Smith, C.J., Watson, C.F., Ray, J., Bird, C.R., Morris, P.C., Schuch, W. and Grierson, D. (1988) Antisense RNA inhibition of polygalacturonase genes in transgenic tomatos. Nature, 334, 724-726.
48. Stitt, M. and Sonnewald, U. (1995) Regulation of metabolism transgenic plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 341-368.
49. Stoutjesdijk, P.A., Singh, S.P., Liu, Q., Hurlstone, C.J., Waterhouse, P.M. and Green, A.G. (2002) hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiol. 129, 1723-1731.
50. Sweetman, J.P., Chu, C., Qu, N., Greenland, A.J., Sonnewald, U. and Jepson, I. (2002) Ethanol vapor is an efficient inducer of the alc gene expression system in model and crop plant species. Plant Physiol. 129, 943-948.
51. Tang, G., Reinhart, B.J., Bartel, D.P. and Zamore, P.D. (2003) A biochemical framework for RNA silencing in plants. Genes Dev. 17, 49-63.
52. The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature, 408, 796-815.
53. Turnage, M.A., Muangsan, N., Peele, C.G. and Robertson, D. (2002) Geminivirus-based vectors for gene silencing in Arabidopsis. Plant J. 30, 107-114.
54. Vance, V. and Vaucheret, H. (2001) RNA silencing in plants - defense and counterdefense. Science, 292, 2277-2280.
55. Voinnet, O., Vain, P., Angell, S. and Baulcombe, D. (1998) Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell, 95, 177-187.
56. Waterhouse, P.M., Graham, H.W. and Wang, M.B. (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl. Acad. Sci. USA, 95, 13959-13964.
57. Yu, J., Hu, S., Wang, J. et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science, 296, 79-91.
58. Zamore, P.D., Tuschl, T., Sharp, P.A. and Bartel, D.P. (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21-23 nucleotide intervals. Cell, 101, 25-33.