Selection and characterization of scFv antibodies against the Sin Nombre hantavirus nucleocapsid protein

Journal of Immunological Methods

Volumn 321, Issue 1-2, 2007, Pages 60-69

  Velappan, Nileena ^a   Martinez, Jennifer S ^a   Valero, Rosa ^a   Chasteen, Leslie ^a   Ponce, Liana ^b   Bondu Hawkins, Virginie ^b   Kelly, Craig ^b   Pavlik, Peter ^a   Hjelle, Brian ^b   Bradbury, Andrew R M ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

Author keywords

Hantavirus; Nucleocapsid protein; Phage antibody; Phage display; scFv; Sin Nombre Virus

Indexed keywords

ALKALINE PHOSPHATASE; HYBRID PROTEIN; NUCLEOCAPSID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY DETECTION; ANTIBODY LIBRARY; ARTICLE; CROSS REACTION; NONHUMAN; PRIORITY JOURNAL; SIN NOMBRE VIRUS;

ALKALINE PHOSPHATASE; ANIMALS; ANTIBODIES, VIRAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HANTAVIRUS PULMONARY SYNDROME; IMMUNOGLOBULIN VARIABLE REGION; NUCLEOCAPSID PROTEINS; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SIN NOMBRE VIRUS; VERO CELLS;

HANTAVIRUS; RODENTIA; SIN NOMBRE VIRUS;

EID: 33947539526     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.01.011     Document Type: Article

References (66)

1. Alfadhli A., Love Z., Arvidson B., Seeds J., Willey J., and Barklis E. Hantavirus nucleocapsid protein oligomerization. J. Virol. 75 (2001) 2019
2. Alfadhli A., Steel E., Finlay L., Bachinger H.P., and Barklis E. Hantavirus nucleocapsid protein coiled-coil domains. J. Biol. Chem. 277 (2002) 27103
3. Alminaite A., Halttunen V., Kumar V., Vaheri A., Holm L., and Plyusnin A. Oligomerization of hantavirus nucleocapsid protein: analysis of the N-terminal coiled-coil domain. J. Virol. 80 (2006) 9073
4. Amersdorfer P., and Marks J.D. Phage libraries for generation of anti-botulinum scFv antibodies. Methods Mol. Biol. 145 (2000) 219
5. Amersdorfer P., Wong C., Smith T., Chen S., Deshpande S., Sheridan R., and Marks J.D. Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries. Vaccine 20 (2002) 1640
6. Ames R.S., Tornetta M.A., Jones C.S., and Tsui P. Isolation of neutralizing anti-C5a monoclonal antibodies from a filamentous phage monovalent Fab display library. J. Immunol. 152 (1994) 4572
7. Ames R.S., Tornetta M.A., Deen K., Jones C.S., Swift A.M., and Ganguly S. Conversion of murine Fabs isolated from a combinatorial Phage display library to full length immunoglobulins. J. Immunol. Methods 184 (1995) 177
8. Ames R.S., Tornetta M.A., McMillan L.J., Kaiser K.F., Holmes S.D., Appelbaum E., Cusimano D.M., Theisen T.W., Gross M.S., Jones C.S., et al. Neutralizing murine monoclonal antibodies to human IL-5 isolated from hybridomas and a filamentous phage Fab display library. J. Immunol. 154 (1995) 6355
9. Barbas C.F.R., Hu D., Dunlop N., Sawyer L., Cababa D., Hendry R.M., Nara P.L., and Burton D.R. In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity. Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 3809
10. Bharadwaj M., Nofchissey R., Goade D., Koster F., and Hjelle B. Humoral immune responses in the hantavirus cardiopulmonary syndrome. J. Infect. Dis. 182 (2000) 43
11. Boder E.T., Midelfort K.S., and Wittrup K.D. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 10701
12. Burton D.R., Barbas C.F., Persson M.A.A., Koenig S., Chanock R.M., and Lerner R.A. A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic individuals. Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 10134
13. Cardoso D.F., Nato F., England P., Ferreira M.L., Vaughan T.J., Mota I., Mazie J.C., Choumet V., and Lafaye P. Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library. Scand. J. Immunol. 51 (2000) 337
14. Casey J.L., Coley A.M., Tilley L.M., and Foley M. Green fluorescent antibodies: novel in vitro tools. Protein Eng. 13 (2000) 445
15. Coia G., Hudson P.J., and Irving R.A. Protein affinity maturation in vivo using E. coli mutator cells. J. Immunol. Methods 251 (2001) 187
16. Daugherty P.S., Chen G., Olsen M.J., Iverson B.L., and Georgiou G. Antibody affinity maturation using bacterial surface display. Protein Eng. 11 (1998) 825
17. de Haard H.J., van Neer N., Reurs A., Hufton S.E., Roovers R.C., Henderikx P., de Bruine A.P., Arends J.W., and Hoogenboom H.R. A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies. J. Biol. Chem. 274 (1999) 18218
18. de Kruif J., and Logtenberg T. Leucine zipper dimerized bivalent and bispecific scFv antibodies from a semi-synthetic antibody phage display library. J. Biol. Chem. 271 (1996) 7630
19. de Kruif J., Boel E., and Logtenberg T. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions. J. Mol. Biol. 248 (1995) 97
20. Grandien M. Viral diagnosis by antigen detection techniques. Clin. Diagn. Virol. 5 (1996) 81
21. Green W., Feddersen R., Yousef O., Behr M., Smith K., Nestler J., Jenison S., Yamada T., and Hjelle B. Tissue distribution of hantavirus antigen in naturally infected humans and deer mice. J. Infect. Dis. 177 (1998) 1696
22. Griep R.A., van Twisk C., Kerschbaumer R.J., Harper K., Torrance L., Himmler G., van der Wolf J.M., and Schots A. pSKAP/S: an expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins. Protein Expr. Purif. 16 (1999) 63
23. Griffiths A.D., Williams S.C., Hartley O., Tomlinson I.M., Waterhouse P., Crosby W.L., Kontermann R.E., Jones P.T., Low N.M., Alison T.J., Prospero T.D., Hoogenboom H.R., Nissim A., Cox J.P.L., Harrison J.L., Zaccolo M., Gherardi E., and Winter G. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13 (1994) 3245
24. Han Z., Karatan E., Scholle M.D., McCafferty J., and Kay B.K. Accelerated screening of phage-display output with alkaline phosphatase fusions. Comb. Chem. High Throughput Screen 7 (2004) 55
25. Hanes J., Schaffitzel C., Knappik A., and Pluckthun A. Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display. Nat. Biotechnol. 18 (2000) 1287
26. Hanke T., Szawlowski P., and Randall R.E. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73 (1992) 653
27. Hatzakis A., Magiorkinis E., and Haida C. HBV virological assessment. J. Hepatol. 44 (2006) S71
28. Hink M.A., Griep R.A., Borst J.W., van Hoek A., Eppink M.H., Schots A., and Visser A.J. Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein. J. Biol. Chem. 275 (2000) 17556
29. Hjelle B. Vaccines against hantaviruses. Expert Rev. Vaccines 1 (2002) 373
30. Hjelle B., Jenison S., Torrez-Martinez N., Herring B., Quan S., Polito A., Pichuantes S., Yamada T., Morris C., Elgh F., Lee H.W., Artsob H., and Dinello R. Rapid and specific detection of Sin Nombre virus antibodies in patients with hantavirus pulmonary syndrome by a strip immunoblot assay suitable for field diagnosis. J. Clin. Microbiol. 35 (1997) 600
31. Iweala O.I. HIV diagnostic tests: an overview. Contraception 70 (2004) 141
32. Jenison S., Yamada T., Morris C., Anderson B., Torrez-Martinez N., Keller N., and Hjelle B. Characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. J. Virol. 68 (1994) 3000
33. Kaukinen P., Kumar V., Tulimaki K., Engelhardt P., Vaheri A., and Plyusnin A. Oligomerization of Hantavirus N protein: C-terminal alpha-helices interact to form a shared hydrophobic space. J. Virol. 78 (2004) 13669
34. Knappik A., Ge L., Honegger A., Pack P., Fischer M., Wellnhofer G., Hoess A., Wolle J., Pluckthun A., and Virnekas B. Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J. Mol. Biol. 296 (2000) 57
35. Kortt A.A., Dolezal O., Power B.E., and Hudson P.J. Dimeric and trimeric antibodies: high avidity scFvs for cancer targeting. Biomol. Eng. 18 (2001) 95
36. Kramer R.A., Marissen W.E., Goudsmit J., Visser T.J., Clijsters-Van der Horst M., Bakker A.Q., de Jong M., Jongeneelen M., Thijsse S., Backus H.H., Rice A.B., Weldon W.C., Rupprecht C.E., Dietzschold B., Bakker A.B., and de Kruif J. The human antibody repertoire specific for rabies virus glycoprotein as selected from immune libraries. Eur. J. Immunol. 35 (2005) 2131
37. Krebber A., Bornhauser S., Burmester J., Honeggar A., Willuda J., H.R.,B., and Pluckthun A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods 201 (1997) 35
38. Marks J.D., Hoogenboom H.R., Bonnert T.P., McCafferty J., Griffiths A.D., and Winter G. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222 (1991) 581
39. Moulard M., Phogat S.K., Shu Y., Labrijn A.F., Xiao X., Binley J.M., Zhang M.Y., Sidorov I.A., Broder C.C., Robinson J., Parren P.W., Burton D.R., and Dimitrov D.S. Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120-CD4-CCR5 complexes. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 6913
40. Muller B.H., Chevrier D., Boulain J.C., and Guesdon J.L. Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization. J. Immunol. Methods 227 (1999) 177
41. Nichol S.T., Spiropoulou C.F., Morzunov S., Rollin P.E., Ksiazek T.G., Feldmann H., Sanchez A., Childs J., Zaki S., and Peters C.J. Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science 262 (1993) 914
42. Nissim A., Hoogenboom H.R., Tomlinson I.M., Flynn G., Midgley C., Lane D., and Winter G. Antibody fragments from a 'single pot' phage display library as immunochemical reagents. EMBO J. 13 (1994) 692
43. Nowakowski A., Wang C., Powers D.B., Amersdorfer P., Smith T.J., Montgomery V.A., Sheridan R., Blake R., Smith L.A., and Marks J.D. Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 11346
44. Olding-Stenkvist E., and Bjorvatn B. Rapid detection of measles virus in skin rashes by immunofluorescence. J. Infect. Dis. 134 (1976) 463
45. Orum H., Andersen P.S., Oster A., Johansen L.K., Riise E., Bjornvad M., Svendsen I., and Engberg J. Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage. Nucleic Acids Res. 21 (1993) 4491
46. Park S.G., Jeong Y.J., Lee Y.Y., Kim I.J., Seo S.K., Kim E.J., Jung H.C., Pan J.G., Park S.J., Lee Y.J., Kim I.S., and Choi I.H. Hepatitis B virus-neutralizing anti-pre-S1 human antibody fragments from large naive antibody phage library. Antivir. Res. 68 (2005) 109
47. Persic L., Roberts A., Wilton J., Cattaneo A., Bradbury A., and Hoogenboom H. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene 187 (1997) 9
48. Rawlinson W., and Scott G. Cytomegalovirus. A common virus causing serious disease. Aust. Fam. Physician 32 (2003) 789
49. Sanna P.P., Williamson R.A., De Logu A., Bloom F.E., and Burton D.R. Directed selection of recombinant human monoclonal antibodies to herpes simplex virus glycoproteins from phage display libraries. Proc. Natl. Acad. Sci. U. S. A. 92 (1995) 6439
50. Sblattero D., and Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat. Biotechnol. 18 (2000) 75
51. Schier R., and Marks J.D. Efficient in vitro affinity maturation of phage antibodies using BIAcore guided selections. Hum. Antib. Hybridomas 7 (1996) 97
52. Schmaljohn C., and Hjelle B. Hantaviruses: a global disease problem. Emerg. Infect. Dis. 3 (1997) 95
53. Schmidt J., Meisel H., Hjelle B., Kruger D.H., and Ulrich R. Development and evaluation of serological assays for detection of human hantavirus infections caused by Sin Nombre virus. J. Clin. Virol. 33 (2005) 247
54. Seme K., Poljak M., Babic D.Z., Mocilnik T., and Vince A. The role of core antigen detection in management of hepatitis C: a critical review. J. Clin. Virol. 32 (2005) 92
55. Sheets M.D., Amersdorfer P., Finnern R., Sargent P., Lindqvist E., Schier R., Hemmingsen G., Wong C., Gerhart J.C., and Marks J.D. Efficient construction of a large nonimmune phage antibody library; the production of panels of high affinity human single-chain antibodies to protein antigens. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 6157
56. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207
57. VanAntwerp J.J., and Wittrup K.D. Fine affinity discrimination by yeast surface display and flow cytometry. Biotechnol. Prog. 16 (2000) 31
58. van den Brink E.N., Ter Meulen J., Cox F., Jongeneelen M.A., Thijsse A., Throsby M., Marissen W.E., Rood P.M., Bakker A.B., Gelderblom H.R., Martina B.E., Osterhaus A.D., Preiser W., Doerr H.W., de Kruif J., and Goudsmit J. Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus. J. Virol. 79 (2005) 1635
59. Vapalahti O., Lundkvist A., Kallio-Kokko H., Paukku K., Julkunen I., Lankinen H., and Vaheri A. Antigenic properties and diagnostic potential of puumala virus nucleocapsid protein expressed in insect cells. J. Clin. Microbiol. 34 (1996) 119
60. Vaughan T.J., Williams A.J., Pritchard K., Osbourn J.K., Pope A.R., Earnshaw J.C., McCafferty J., Hodits R.A., Wilton J., and Johnson K.S. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14 (1996) 309
61. Williamson R.A., Burioni R., Sanna P.P., Partridge L.J., Barbas C.F., and Burton D.R. Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries. Proc. Natl. Acad. Sci. U. S. A. 90 (1993) 4141
62. Yoshimatsu K., Lee B.H., Araki K., Morimatsu M., Ogino M., Ebihara H., and Arikawa J. The multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes. J. Virol. 77 (2003) 943
63. Zebedee S.L., Barbas C.F., Hom Y., Caothien R.H., Graff R., Degraw J., Pyati J., LaPolla R., Burton D.R., Lerner R.A., and Thronton G.B. Human combinatorial antibody libraries to hepatitis B surface antigen. Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 3175
64. Zhang M.Y., Shu Y., Phogat S., Xiao X., Cham F., Bouma P., Choudhary A., Feng Y.R., Sanz I., Rybak S., Broder C.C., Quinnan G.V., Evans T., and Dimitrov D.S. Broadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library. J. Immunol. Methods 283 (2003) 17
65. Zhang J., Tanha J., Hirama T., Khieu N.H., To R., Tong-Sevinc H., Stone E., Brisson J.R., and MacKenzie C.R. Pentamerization of single-domain antibodies from phage libraries: a novel strategy for the rapid generation of high-avidity antibody reagents. J. Mol. Biol. 335 (2004) 49
66. Zvirbliene A., Samonskyte L., Gedvilaite A., Voronkova T., Ulrich R., and Sasnauskas K. Generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles. J. Immunol. Methods 311 (2006) 57