Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization

Journal of Immunological Methods

Volumn 227, Issue 1-2, 1999, Pages 177-185

  Muller, Bruno H ^a,b   Chevrier, Danièle ^a   Boulain, Jean Claude ^b   Guesdon, Jean Luc ^a  

^a INSTITUT PASTEUR   (France)

^b CEA SACLAY   (France)

Author keywords

Enzyme immunodetection, 2 Acetylaminofluorene; Fusion protein; Molecular hybridization; Recombinant scFv PhoAv conjugate, Phage display

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL DNA; DNA; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; MONOCLONAL ANTIBODY; N (2 FLUORENYL)ACETAMIDE; NUCLEIC ACID; REAGENT;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; DOT HYBRIDIZATION; ENZYME IMMUNOASSAY; ESCHERICHIA COLI; MOLECULAR HYBRIDIZATION; MOUSE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; RNA SEQUENCE;

2-ACETYLAMINOFLUORENE; ALKALINE PHOSPHATASE; ANIMALS; CELL LINE; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN FRAGMENTS; MICE; NUCLEIC ACID HYBRIDIZATION; RECOMBINANT FUSION PROTEINS; RNA;

ANIMALIA; BACTERIA (MICROORGANISMS); CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; ESCHERICHIA COLI;

EID: 0032817913     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00071-X     Document Type: Article

References (30)

1. Auriol J., Guesdon J.-L., Mazié J.-C., Nato F. Development of a bispecific monoclonal antibody for use in molecular hybridisation. J. Immunol. Methods. 169:1994;123.
2. Boulain, J.-C., Cattolico, L., Ducancel, F., Ménez, A., 1995. Phosphatases alcalines bactériennes modifiées et leurs applications. French Patent Application 95.07833.
3. Brodeur P.H., Riblet R. The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse: I. One hundred Igh-V genes comprise seven families of homologous genes. Eur. J. Immunol. 14:1984;922.
4. Carrier A., Ducancel F., Settiawan N.B., Cattolico L., Maillère B., Léonetti M., Drevet P., Ménez A., Boulain J.-C. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection. J. Immunol. Methods. 181:1995;177.
5. Chanussot C., Bellanger L., Ligny-Lemaire C., Seguin P., Ménez A., Boulain J.-C. Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin. J. Immunol. Methods. 197:1996;39.
6. Chesneau O., Aubert S., Morvan A., Guesdon J.-L., El Solh N. Usefulness of the ID32 staph system and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates. J. Clin. Microbiol. 30:1992;2346.
7. Coleman J.E. Structure and mechanism of alkaline phosphatase. Annu. Rev. Biophys. Biomol. Struct. 21:1992;441.
8. Ducancel F., Gillet D., Carrier A., Lajeunesse E., Ménez A., Boulain J.-C. Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli. Biotechnology. 11:1993;601.
9. Fuchs R., Daune M. Physical studies on deoxyribonucleic acid after covalent binding of a carcinogen. Biochemistry. 11:1972;2659.
10. Gillet D., Ducancel F., Pradel E., Léonetti M., Ménez A., Boulain J.-C. Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities. Protein Eng. 5:1992;273.
11. Gillet D., Ezan E., Ducancel F., Gaillard C., Ardouin T., Istin M., Ménez A., Boulain J.-C., Grognet J.-M. Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer. Anal. Chem. 65:1993;1779.
12. Grimont F., Grimont P.A.D. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann. Inst. Pasteur/Microbiol. 137B:1986;165.
13. Grimont F., Chevrier D., Grimont P.A.D., Lefèvre M., Guesdon J.-L. Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy. Res. Microbiol. 140:1989;447.
14. Guesdon J.-L. Immunoenzymatic techniques applied to the specific detection of nucleic acids. A review. J. Immunol. Methods. 150:1992;33.
15. Ho S.N., Hunt H.D., Horton R.M., Pullen J.K., Pease L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 77:1989;51.
16. Kabat, E.A., Wu, T.T., Reid-Miller, M., Perry, H.M., Gottesman, K.S., 1987. Sequences of proteins of immunological interest. National Institute of Health, Bethesda, MD.
17. Kourilsky, P., Avrameas, S., Cami, B., Guesdon, J.-L., 1978. French Patent Application 78.10975.
18. Kricka, L.J., 1992. Nonisotopic DNA Probe Techniques. Academic Press, San Diego, CA.
19. Le Guern A., Leng M., Kourilsky P. Monoclonal antibodies to DNA modified by the carcinogen N-acetoxy-N-2 acetyl aminofluorene. Dev. Biol. Stand. 57:1984;409.
20. Mahana W., Matsiota P., Avrameas S. Both natural and induced murine anti-TNP antibodies possess anti-DNA activity. Ann. Inst. Pasteur/Immunol. 138:1987;805.
21. Marks J.D., Hoogenboom H.R., Bonnert T.P., McCafferty J., Griffiths A.D., Winter G. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222:1991;581.
22. Masse M.J., Meulien P., Le Guern A., Kourilsky P. Monoclonal antibody detection of 2-acetyl-aminofluorene-modified DNA probes for the specific detection of nucleic acids in hybridization procedures. Ann. Inst. Pasteur/Immunol. 136:1985;231.
23. Mérienne K., Germain N., Zinn-Justin S., Boulain J.-C., Ducancel F., Ménez A. The functional architecture of an acetylcholine receptor-mimicking antibody. J. Biol. Chem. 272:1997;23775.
24. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
25. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:1977;5463.
26. Southern E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:1975;503.
27. Strohal R., Helmberg A., Kroemer G., Kofler R. Mouse Vκ gene classification by nucleic acid sequence similarity. Immunogenetics. 30:1989;475.
28. Tchen P., Fuchs R.P.P., Sage E., Leng M. Chemically modified nucleic acids as immunodetectable probes in hybridization experiments. Proc. Natl. Acad. Sci. U.S.A. 81:1984;3466.
29. Tonegawa S. Somatic generation of antibody diversity. Nature. 302:1983;575.
30. Wels W., Harwerth I.M., Zwickl M., Hardman N., Groner B., Hynes N.E. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor. Biotechnology. 10:1992;1128.