Effects of TGF-β and glucocorticoids on map kinase phosphorylation, IL-6/IL-11 secretion and cell proliferation in primary cultures of human lung fibroblasts

Journal of Cellular Physiology

Volumn 210, Issue 2, 2007, Pages 489-497

  Pelaia, Girolamo ^a   Gallelli, Luca ^a   D'Agostino, Bruno ^b   Vatrella, Alessandro ^b   Cuda, Giovanni ^a   Fratto, Donatella ^a   Renda, Teresa ^a   Galderisi, Umberto ^b   Piegari, Elena ^b   Crimi, Nunzio ^c   Rossi, Francesco ^b   Caputi, Mario ^b   Costanzo, Francesco S ^a   Vancheri, Carlo ^c   Maselli, Rosario ^a   Marsico, Serafino A ^b  

^a UNIVERSITY MAGNA GRAECIA OF CATANZARO   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

^c UNIVERSITY OF CATANIA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BUDESONIDE; GLUCOCORTICOID; GROWTH FACTOR; INTERLEUKIN 11; INTERLEUKIN 6; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE INHIBITOR; THYMIDINE; TRANSFORMING GROWTH FACTOR BETA; TRYPAN BLUE;

ARTICLE; ASTHMA; CELL ASSAY; CELL CULTURE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; FUNCTIONAL PROTEOMICS; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERSTITIAL LUNG DISEASE; LUNG FIBROBLAST; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; STAINING; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; SUPERNATANT; WESTERN BLOTTING;

CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; ENZYME ACTIVATION; ENZYME INHIBITORS; FIBROBLASTS; GLUCOCORTICOIDS; HUMANS; INTERLEUKIN-11; INTERLEUKIN-6; INTERLEUKINS; LUNG; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; PHOSPHORYLATION; TRANSFORMING GROWTH FACTOR BETA1; UP-REGULATION;

EID: 33845670432     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.20884     Document Type: Article

References (50)

1. Bamba S, Andoh A, Yasui H, Makino J, Kim S, Fujiyama Y. 2003. Regulation of IL-11 expression in intestinal myofibroblasts: Role of c-Jun AP-1 and MAPK-dependent pathways. Am J Physiol Gastrointest Liver Physiol 285:G529-G538.
2. Barnes PJ. 2006. Corticosteroid effects on cell signalling. Eur Respir J 27:413-426.
3. Barnes PJ, Shapiro SD, Pauwels RA. 2003. Chronic obstructive pulmonary disease: Molecular and cellular mechanisms. Eur Respir J 22:672-688.
4. Black PN, Young PG, Skinner SJ. 1996. Response of airway smooth muscle cells to TGF-beta1: Effects on growth and synthesis of glycosaminoglycans. Am J Physiol 271:L910-L917.
5. Boxall C, Holgate ST, Davies DE. 2006. The contribution of transforming growth factor-β and epidermal growth factor signalling to airway remodelling in chronic asthma. Eur Respir J 27:208-229.
6. Chakir J, Shannon J, Molet S, Fukakusa M, Elias J, Laviolette M, Boulet L-P, Hamid K. 2003. Airway remodeling-associated mediators in moderate to severe asthma; effects of steroids on TGF-β, IL-11, IL-17, and type I and type III collagen expression. J Allergy Clin Immunol 111:1293-1298.
7. Chang L, Karin M. 2001. Mammalian MAP kinase signalling cascades. Nature 410:37-40.
8. Chen G, Khalil N. 2006. TGF-β1 increases proliferation of airway smooth muscle cells by phosphorylation of MAP kinases. Respir Res doi: 10.1186/1465-9921-7-2.
9. Crestani B, Seta N, De Bandt M, Soler P, Rolland C, Dehoux M, Boutien A, Dombret MC, Palazzo E, Kahn MF. 1994. Interleukin-6 secretion by monocytes and alveolar macrophages in systemic sclerosis with lung involvement. Am J Respir Crit Care Med 149:1260-1265.
10. Elias JA, Zheng T, Whiting NL, Trow TK, Merrill WW, Zitnik R, Ray P, Alderman EM. 1994. IL-11 and transforming growth factor-beta regulation of fibroblast-derived IL-11. J Immunol 152:2421-2429.
11. Elias JA, Zhu Z, Chupp G, Homer RJ. 1999. Airway remodeling in asthma. J Clin Invest 104:1001-1006.
12. Finlay GA, Thannickal VJ, Fanburg BL, Paulson KE. 2000. Transforming growth factor-beta1-induced activation of the ERK pathway/activator protein-1 in human lung fibroblasts requires the autocrine induction of basic fibroblast growth factor. J Biol Chem 8:27650-27656.
13. Fitzgerald SM, Lee SA, Hall K, Chi DS, Krishnaswamy G. 2004. Human lung fibroblasts express IL-6 in response to signaling following mast cell contact. Am J Respir Cell Mol Biol 30:585-593.
14. A receptor-dependent activation of MAP kinases. J Cell Biochem 96:858-868.
15. Hagimoto N, Kuwano K, Inoshima I, Yoshimi M, Nakamura N, Fujita M, Maeyama T, Hara N. 2002. TGF-β1 as an enhancer of Fas-mediated apoptosis of lung epithelial cells. J Immunol 168:6470-6478.
16. Hansson A, Hehenberger K, Thoren M. 1996. Long-term treatment of Swiss 3T3 fibroblasts with dexamethasone attenuates MAP kinase activation induced by insulin-like growth factor-I (IGF-I). Cell Biochem Funct 14:121-129.
17. 1 induces phenotypic modulation of human lung fibroblast to myofibroblast through c-Jun-NH2-terminal kinase-dependent pathway. Am J Respir Crit Care Med 163:152-157.
18. Hogg JC, Chu F, Utokaparch S, Woods R, Elliott WM, Buzatu L, Cherniack RM, Rogers RM, Sciurba FC, Coxson HO, Pare PD. 2004. The nature of small airway obstruction in chronic obstructive pulmonary disease. N Engl J Med 350:2645-2653.
19. Horowitz JC, Lee DY, Waghray M, Keshamouni VG, Thomas PE, Zhang H, Cui Z, Thannickal VJ. 2004. Activation of the pro-survival phosphatidylinositol 3-kinase/AKT pathway by transforming growth factor-beta1 in mesenchymal cells is mediated by p38 MAPK-dependent induction of an autocrine growth factor. J Biol Chem 279:1359-1367.
20. Hoshino M, Nakamura Y, Sim JJ, Yamashiro Y, Uchida K, Hosaka K, Isogai S. 1998. Inhaled corticosteroid reduced lamina reticularis of the basement membrane by modulation of insulin-like growth factor (IGF)-1 expression in bronchial asthma. Clin Exp Allergy 28:568-577.
21. Jordana M, Schulman J, McSharry C, Gauldie J. 1988. Heterogenous proliferative characteristics of human adult lung fibroblasts from control and fibrotic tissue. Am Rev Respir Dis 137:579-584.
22. Kelly M, Kolb M, Bonniaud P, Gauldie J. 2003. Re-evaluation of fibrogenic cytokines in lung fibrosis. Curr Pharm Des 9:39-49.
23. Khalil N, O'Connor RN, Flanders KC, Unruh H. 1996. TGF-β1, but not TGF-β2 or TGF-β3, is differentially present in epithelial cells of advanced pulmonary fibrosis: An immunohistochemical study. Am J Respir Cell Mol Biol 14:131-138.
24. Khalil N, Xu YD, O'Connor R, Duronio V. 2005. Proliferation of pulmonary interstitial fibroblasts is mediated by transforming growth factor-β1- induced release of extracellular fibroblast growth factor-2 and phosphorylation of p38 MAPK and JNK. J Biol Chem 280:43000-43009.
25. 1. J Exp Med 194:809-821.
26. McMillan SJ, Xanthou G, Lloyd CM. 2005. Therapeutic administration of budesonide ameliorates allergen-induced airway remodeling. Clin Exp Allergy 35:388-396.
27. Minshall E, Chakir J, Laviolette M, Molet S, Zhu Z, Olivenstein R, Elias JA, Hamid Q. 2000. IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils. J Allergy Clin Immunol 105:232-238.
28. Moodley YP, Scaffidi AK, Misso NL, Keerthisingam C, McAnulty RJ, Laurent GJ, Mutsaers SE, Thompson PJ, Knight DA. 2003. Fibroblasts isolated from normal lungs and those with idiopathic pulmonary fibrosis differ in interleukin-6/gp 130-mediated cell signaling and proliferation. Am J Pathol 163:345-354.
29. Olivieri D, Chetta A, Del Donno A, Bertorelli G, Canalini A, Pesci A, Testi R, Foresi A. 1997. Effect of short-term treatment with low-dose inhaled fluticasone propionate on airway inflammation and remodeling in mild asthma: A placebo-controlled study. Am J Respir Crit Care Med 155:1864-1871.
30. Pelaia G, Cuda G, Vatrella A, Grembiale RD, De Sarro GB, Maselli R, Costanzo FS, Avvedimento VE, Rotiroti D, Marsico SA. 2001. Effects of glucocorticoids on activation of c-Jun N-terminal, extracellular signal-regulated, and p38 MAP kinases in human pulmonary endothelial cells. Biochem Pharmacol 62:1719-1724.
31. Pelaia G, Cuda G, Vatrella A, Grembiale RD, Fratto D, Tagliaferri P, Maselli R, Costanzo FS, Marsico SA. 2003. Effects of transforming growth factor-β and budesonide on mitogen-activated protein kinase activation and apoptosis in airway epithelial cells. Am J Respir Cell Mol Biol 29:12-18.
32. 4 induces TGF-β1 production in airway epithelium via p38 kinase pathway. Am J Respir Cell Mol Biol 34:101-107.
33. Pulleyn LJ, Newton R, Adcock IM, Barnes PJ. 2001. TGFβ1 allele association with asthma severity. Hum Genet 109:623-627.
34. Redington AE, Madden J, Frew AJ, Djukanovic R, Roche WR, Holgate ST, Howarth PH. 1997. Transforming growth factor-β1 in asthma: Measurement in bronchoalveolar lavage. Am J Respir Crit Care Med 156:642-647.
35. Shahar I, Fireman E, Topilsky M, Grief J, Kivity S, Spirer Z, Ben Efraim S. 1996. Effect of IL-6 on alveolar fibroblast proliferation in interstitial lung diseases. Clin Immunol Immunopathol 79:244-251.
36. Silverman ES, Palmer LJ, Subramaniam V, Hallock A, Mathew S, Vallone J, Faffe DS, Shikanai T, Raby BA, Weiss ST, Shore SA. 2004. Transforming growth factor-beta1 promoter polymorphism C-509T is associated with asthma. Am J Respir Crit Care Med 169:214-219.
37. Song CZ, Tian X, Gelehrter TD. 1999. Glucocorticoid receptor inhibits transforming growth factor-β signaling by directly targeting the transcriptional activation function of Smad3. Proc Natl Acad Sci USA 96:11776-11781.
38. Taga T. 1997. gp130 and the interleukin-6 family of cytokines. Annu Rev Immunol 15:797-819.
39. Takizawa H, Tanaka M, Takami K, Ohtoshi T, Ito K, Satoh M, Okada Y, Yamasawa F, Nakahara K, Umeda A. 2001. Increased expression of transforming growth factor-β1 in small airway epithelium from tobacco smokers and patients with chronic obstructive pulmonary disease (COPD). Am J Respir Crit Care Med 163:1476-1483.
40. Tang W, Yang L, Yang Y-C, Leng SX, Elias JA. 1998. Transforming growth factor-β stimulates interleukin-11 transcription via complex activating protein-1-dependent pathways. J Biol Chem 273:5506-5513.
41. Trigg CJ, Manolitsas ND, Wang J, Calderon MA, McAulay A, Jordan SE, Herdman MJ, Jhalli N, Duddle JM, Hamilton SA, Devalia JL, Davies RJ. 1994. Placebo-controlled immunopathologic study of four months of inhaled corticosteroids in asthma. Am J Respir Crit Care Med 150:17-22.
42. van den Bosch JMM, Westermann C, Aumann J, Edsbacker S, M. Ton-nesson M, Selroos O. 1993. Relationship between lung tissue and blood concentrations of inhaled budesonide. Biopharm Drug Dispos 14:455-459.
43. Vancheri C, Sortino MA, Tomaselli V, Mastruzzo C, Condorelli F, Bellistri G, Pistorio MP, Canonico PL, Crimi N. 2000. Different expression of TNF-α receptors and prostaglandin E2 production in normal and fibrotic lung fibroblasts: Potential implications for the evolution of the inflammatory process. Am J Respir Cell Mol Biol 22:628-634.
44. Vivien D, Galera P, Lebrun E, Loyau G, Pujol JP. 1990. Differential effects of transforming growth factor-beta and epidermal growth factor on the cell cycle of cultured rabbit articular chondrocytes. J Cell Physiol 143:834-845.
45. Wang J, Zhu Z, Nolfo R, Elias JA. 1999. Dexamethasone regulation of lung epithelial cell and fibroblast interleukin-11 production. Am J Physiol Lung Cell Mol Physiol 276:L175-L185.
46. Wen FQ, Kohyama T, Skold CM, Zhu YK, Liu X, Romberger DJ, Stoner J, Rennard SI. 2002. Glucocorticoids modulate TGF-beta production. Inflammation 26:279-290.
47. Widmann C, Gibson S, Jarpe MB, Johnson GL. 1999. Mitogen-activated protein kinase: Conservation of a three kinase module from yeast to human. Physiol Rev 79:143-180.
48. Winzen R, Kracht M, Ritter B, Wilhelm A, Chen C-YA, Shyu A-B, Muller M, Gaestel M, Reach K, Holtmann H. 1999. The p38 MAP kinase pathway signals for cytokine-induced mRNA stabilization via MAP kinase-activated protein kinase 2 and an AU-rich region-targeted mechanism. EMBO J 18:4969-4980.
49. Yoshida K, Kuwano K, Hagimoto N, Watanabe K, Matsuba T, Fujita M, Inoshima I, Hara N. 2002. MAP kinase activation and apoptosis in lung tissues from patients with idiopathic pulmonary fibrosis. J Pathol 198:388-396.
50. Zhu Z, Lee CG, Zheng T, Chupp G, Wang J, Homer RJ, Noble PW, Hamid Q, Elias JA. 2001. Airway inflammation and remodeling in asthma: Lessons from interleukin 11 and interleukin 13 transgenic mice. Am J Respir Crit Care Med 164:S67-S70.