Genetics, epigenetics and gene silencing in differentiating mammalian embryos

Reproductive BioMedicine Online

Volumn 13, Issue 5, 2006, Pages 732-753

  Edwards, R G ^a  

^a Dry Drayton   (United Kingdom)

Author keywords

Cloning; Early embryogenesis; Early haemopoietic stages; Gene silencing; Mammalian embryos; Tissue reprogramming

Indexed keywords

BLASTOCYST; CELL DIFFERENTIATION; CELL NUCLEUS TRANSPLANTATION; CLONING; CULTURE MEDIUM; DEVELOPMENTAL STAGE; DNA MODIFICATION; EMBRYO; EMBRYO CULTURE; EMBRYO DEVELOPMENT; EMBRYO GROWTH; EMBRYONIC STEM CELL; EPIGENETICS; FERTILIZATION; FERTILIZATION IN VITRO; GENE EXPRESSION; GENE SILENCING; GENETIC PROCEDURES; GENETICS; GENOME IMPRINTING; GROWTH CURVE; HEMATOPOIESIS; HISTOGENESIS; HUMAN; IN VITRO STUDY; MAMMAL; MAMMALIAN GENETICS; NONHUMAN; OOCYTE; OOCYTE CLEAVAGE; PREIMPLANTATION EMBRYO; PRENATAL DIAGNOSIS; PROTEOMICS; REVIEW; X CHROMOSOME INACTIVATION;

EID: 33751107771     PISSN: 14726483     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1472-6483(10)60665-7     Document Type: Review

References (172)

1. Akashi K, He X, Chen J et al. 2003 Transcriptional accessibility for genes of multiple tissues and haemopoietic lineages is hierarchically controlled during early haematopoiesis. Blood 101, 383-389.
2. Antczak M, Van Blerkom J 1999 Temporal and spatial effects of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized sites. Molecular Human Reproduction 14, 429-447.
3. Antczak M, Van Blerkom J 1997 Oocyte influences on early development: leptin and STAT3 are polarised in mouse and human oocytes and differentially distributed within the cells of the preimplantation embryo. Molecular Human Reproduction 3, 1067-1086.
4. Arney KL, Bao S, Bannister AJ et al. 2002 Histione methylation defines epigenetic asymmetry in the mouse oocyte. International Journal of Developmental Biology 46, 317-320.
5. Bachman KE, Rountree MR, Schulz RM 2001 Dnmt3a and Drm13b are transcriptional repressors that exhibit unique localization properties to heterochromatin. Journal of Bioological Chemistry 276, 32282-32287.
6. Bailey AS, Jians G, Afentoulis M et al. 2004 Transplanted adult haematopoietic stem cells differentiate into functional endothelial cells. Blood 103, 13-19.
7. Baksh D, Davies JE, Zandstra PW 2005 Soluble cross-talk between human bone marrow derived haematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor compartment. Blood 106, 3012-3020.
8. Bannister AJ, Zegerman P, Partridge JF et al. 2001 Selective recognition of methylated lysine 9 on H3 by the HP1 chromic domain. Nature 410, 120-124.
9. Birnbacher D 2005 Human cloning and human dignity. Reproductive BioMedicine Online 12 (Suppl. 1), 50-55.
10. Boiani M, Eckardt S, Scholer HR, McLaughlin KJ 2002 Oct4 distribution and level in mouse clones: consequences for pluripotency. Genes and Development 16, 1209-1219.
11. Boquest AC, Shahdadfar A, Fronsdal K et al. 2005 Isolation and transcriptional profiling of purified uncultured human stromal stem cells: alteration of gene expression following in vitro cell culture. Molecular Biology of the Cell 16, 1131-1141.
12. Bortvin A, Eggan K, Skaletsky H et al. 2003 Incomplete reactivation of Oct-4 related genes in mouse embryos cloned from somatic nuclei. Development 139, 1673-1680.
13. Brown CJ, Ballabio A, Rupert H et al. 1991 A gene from the region of the human X inactivation centre is expressed exclusively form the inactive X chromosome. Nature 349, 88-44.
14. Buiting K, Dittrich B, Gross S et al. 1998 Sporadic imprinting defects in Prader-Willi and Angelmann syndromes: implications for imprint switch models, genetic counselling and prenatal diagnosis. American Journal of Human Genetics 63, 170-180.
15. Byrne JA, Simonsson S, Western PS et al. 2003 Nuclei of adult mammalian cells are directly reprogrammed to oct-4 stem cells gene expression by amphibian oocytes. Current Biology 13, 1206-1213.
16. Cáceres L, Nilson LA 2005 Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. Development 132, 2345-2353.
17. Campbell KHS. Ritchie WH, Wilmut I 1993 Nuclear-cytoplasmic interactions during the first cell cycle of nuclear transfer reconstructed bovine embryos: implications for deoxyribonucleic acid replication and development. Biology of Reproduction 50, 933-942.
18. Cao YA, Wagers AJ, Beilhack A et al. 2004 Shifting foci of haematopoiesis during reconstitution from single stem cells. Proceedings of the National Academy of Sciences of the USA 101, 221-226.
19. Chadwick BP, Willard HF 2003 SETting the scene: Eed-Enx1 leaves an epigenetic signature in the inactive X chromosome. Developmental Cell 4, 445-447.
20. Cheong H-T, Takahashi Y, Kanagawaw 1993 Birth of mice after transplantation of early cell-cycle stage embryonic nuclei into enucleated oocytes. Biology of Reproduction 48, 958-963.
21. Chesne P, Adenot PG, Viglietta C et al. 2003 Cloned rabbits produced by nuclear transfer from adult somatic cells. Nature Biotechnology 20, 366-369.
22. Choi M, Kennedy M, Kazarov A et al. 1998 A common precursor for haematopoietic and endothelial stem cells. Development 125, 725-732.
23. Cole RJ, Edwards RG, Paul J 1966 Cytodifferentiation and embryogenesis in cell colonies and tissue cultures derived from ova and blastocysts of the rabbit. Developmental Biology 13, 285-307.
24. Collas P, Sullivan EJ, Barnes FL 1993 Histone H1 kinase activity in bovine oocytes following calcium stimulation. Molecular and Reproductive Development 34, 224-231.
25. Cowan CA, Atienza J, Melton DA et al. 2005 Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science 309, 1369-1373.
26. Cox GF, Burger J, Lip V et al. 2002 Intracytoplasmic some injection may increase the risk of imprinting defects. American Journal of Human Genetics 71, 162-164.
27. Deb K, Sivaguru M, Yong HY, Roberts M 2006 Cdx2 expression and trophectoderm lineage specification in mouse embryos. Science 311, 992-996.
28. Donnison M, Beaton A, Davey HW et al. 2005 Loss of the extra-embryonic ectoderm in Elf5 mutants leads to defects in embryonic patterning. Development 132, 2299-2308.
29. Drake CJ, Fleming PA 2000 Vasculogenesis in the day 6.5 to 9.5 mouse embryo. Blood 95, 1671-1679.
30. Drewes G, Ebneth A, Preuss U et al. 1997 MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption. Cell 89, 297-308.
31. D'Souza SL, Elefanty AG, Keller G 2005 SCL/Tal-1 is essential for haematopoietic commitment of the hemangioblast but not for its development. Blood 105, 3862-3870.
32. Duncan AW, Rattis FM, DiMascio L et al. 2005 Integration of Notch and Wnt signalling in haemopoietic stem cell maintenance. Nature Immunology 6, 314-322.
33. Eaton S, Simons K 1995 Atypical, basal and lateral axes for epithelial polarization. Cell, 82, 5-8.
34. Edwards RG 2005a Genetics of polarity in mammalian embryos. Reproductive BioMedicine Online 11, 104-114.
35. Edwards RG 2005b Changing genetic world of IVF, stem cells and PGD. C. Embryogenesis and the differentiation of the haemopoietic system. Reproductive BioMedicine Online 11, 777-785.
36. Edwards RG 2004 Stem cells today. B1 Bone marrow stem cells. Reproductive BioMedicine Online 6, 160-163.
37. Edwards RG, Beard H 1999 Hypothesis: sex determination and germline formation are committed at the pronuclear stage in mammalian embryos. Molecular Human Reproduction 5, 595-606.
38. Edwards RG, Beard HK 1998 How identical would cloned children be? An understanding essential to the ethical debate. Human Reproduction Update 4, 789-811,
39. Edwards RG, Beard H 1997 Oocyte polarity and cell determination in early mammalian embryos. Molecular Human Reproduction 3, 863-905.
40. Edwards RG, Brody SA 1995 Principles and Practice of Assisted Human Reproduction. WB Saunders Company, Philadelphia.
41. Edwards RG, Hansis C 2005 Initial differentiation of blastomeres in 4-cell embryos and its significance for early embryogenesis and implantation. Reproductive BioMedicine Online 11, 206-218.
42. Edwards RG, Sirlin JL 1956 Studies in gametogenesis, fertilization and early development in the mouse using radioactive tracers. Proceedings of the 2nd World Congress on Fertility and Sterility, Naples, pp. 376-386.
43. El-Maarri O, Buiring K, Perry EG et al. 2001 Maternal methylation imprints on human chromosome 11 established at or around the time of fertilization. Nature Genetics 27, 341-344.
44. Ema M, Faloon P, Zhang WJ et al. 2003 Combinatorial effects of Flk1 and Tal1 on vascular and haematopoietic development in the mouse. Genes and Development 17, 380-393.
45. Endoh M, Ogawa M, Orkin S, Nishikawa S 2002 SCL/tal-1-dependent processes determines a competence to select the definitive haemopoietic lineage prior to endothelial differentiation. EMBO Journal 21, 6700-6708.
46. Etienne-Manneville S 2004 Cdc42 - the centre of polarity. Journal of Cell Science 117, 1291-1300.
47. Ferguson-Smith AC, Reik W 2003 The need for Eed. Nature Genetics 33, 433-434.
48. Ferkowitz MJ, Starr M, Xie X et al. 2003 CD41 expression defines the onset of primitive and definitive haematopoiesis in the murine embryo. Development 130, 4393-4403.
49. Fire A, Xu S, Montgomery MK et al. 1998 Potent and specific gene interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811.
50. Fleming TP, Johnson MH 1988 From egg to epithelium. Annual Review of Cell Biology 4, 459-485.
51. Fleming TP, Garrod DR, Elsmore AJ 1991 Desmosome biogenesis in the mouse preimplantation embryo. Development 112, 527-539.
52. Fraga MF, Ballestar E, Paz MF et al. 2005 Epigenetic differences arise during the lifetime of monozygotic twins. Proceedings of the National Academy of Sciences, USA 102, 10604-10609.
53. Fujimori T, Kurotaki Y, Miyazaki I, Nebeshima Y-I 2003 Analysis of cell lineage in two- and four-cell mouse embryos. Development 130, 5113-5122.
54. Galat V, Oren S, Rechitsky S et al. 2005 Cytogenetic analysis of human somatic cell haploidization. Reproductive BioMedicine Online 10, 199-204.
55. Gardner RL 2006 Weaknesses in the case against prepatterning in the mouse. Reproductive BioMedicine Online 12, 144-149.
56. Gardner RL 2001 Specification of embryonic axes begins before cleavage in normal mouse development. Development 128, 839-847.
57. Gardner RL, Davis TJ 2003 Is the plane of first cleavage related to the point of sperm entry in the mouse? Reproductive BioMedicine Online 6, 157-160.
58. Green AR 2005 A tale of two lineages: the origins of blood and endothelium. Blood 105, 3758.
59. Gulyas BJ 1975 A re-examination of cleavage patterns in eutherian mammalian eggs: rotation of blastomere pairs during second cleavage in the rabbit. Journal of Experimental Zoology 193, 235-248.
60. Gunsalus KC, Ge H, Schetter AS et al. 2005 Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis. Nature 436, 861-865.
61. Guo S, Kemphues KJ 1996 Molecular genetics of asymmetric cleavage in the early Caenorhabditis elegans embryo. Current Opinion in Genetics and Development 6, 408-415.
62. Guo S, Kemphues KS 1995 par-1, a gene required for establishing polarity in C. elegans embryos, encodes a Ser/Thr kinase that is asymmetrically distributed. Cell 81, 611-620.
63. Hajkova P, Erhardt S, Lane N et al. 2002 Epigenetic reprogramming in mouse primordial germ cells. Mechanisms in Development 117, 15-23.
64. Hamatani T, Carter MS, Sharov AA, Ko MSH 2003 Dynamics of global gene expression changes during mouse preimplantation development. Developmental Cell 6, 117-131..
65. Handyside AH, Johnson MH 1978 Temporal and spatial patterns of the synthesis of tissue-specific polypeptides in the preimplantation mouse embryo. Journal of Embryology and Experimental Morphology 44, 191-199.
66. Hansis C, Edwards RG 2003 Cell differentiation in the preimplantation human embryo. Reproductive BioMedicine Online 6, 215-220.
67. Hiiragi T, Solter D 2005 Fatal flaws in the case for prepatterning in the mouse egg. Reproductive BioMedicine Online 12, 150-152.
68. Hiiragi T, Solter D 2004 First cleavage plane of the mouse egg is not predetermined but defined by the topology of the two opposing pronuclei. Nature 430, 360-364.
69. Hinds HL, Ashley CT, Sutckiffe JS et al. 1993 Tissue specific expression of FMR-1 provides evidence for a functional role in fragile X syndrome. Nature Genetics 3, 36-43.
70. Hochedlinger K, Jaenisch R 2003 Nuclear transplantation, embryonic stem cells and the potential for therapy. New England Journal of Medicine 349, 275-286.
71. Hong J-H, Hwang ES, McManus MT et al. 2005 TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science 309, 1074-1078.
72. Hoshi H 2003 In vitro production of bovine embryos and their application for embryo transfer. Theriogenology 15, 675-685.
73. Huber TL, Koustoff V, Fehling HJ et al. 2004 Haemangioblast commitment is initiated in the primitive stream; of the mouse embryo. Nature 432, 625-630.
74. Hubner K, Fuhmann G, Christensen CR et al. 2003 Derivation of oocytes from mouse embryonic stem cells. Science 300, 1252-1268.
75. Humpherys D, Eggan K, Akutsu H et al. 2001 Abnormal gene expression in cloned mice derived from embryonic stem cells and cumulus nuclei. Proceedings of the National Academies of Science, Washington 99, 1289-1294.
76. Hung TJ, Kemphues KJ 1999 PAR-6 is a conserved PZ domain-containing protein that colocalises with PAR-3 in C. elegans embryos. Development 126, 127-135.
77. Huyhn KD, Lee JT 2003 Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos. Nature 426, 857-862.
78. Izumi Y, Hirose T, Tamai Y et al. 1998 An atypical PKC directly associates and colocalises at the epithelial tight junction with ASII, a mammalian homologue of C. elegans polarity protein PAR-3. Journal of Cell Biology 143, 95-106.
79. Johnson J, Bagley J, Skaznik-Wilkiel M 2005 Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood. Cell 122, 303-315.
80. Johnson J, Canning J, Keneke T et al. 2004 Germline stem cells and follicular renewal in the postnatal mammalian embryo. Nature 428, 145-150.
81. Judson H, Hayward BE, Sheridan E, Bonthron DT 2002 A global disorder of imprinting in the human germline. Nature 418, 539.
82. Kabrun N, Bühring H-J, Choi HK et al. 1997 FLK-1 expression defines a population of early haematopoietic precursors. Development 124, 2039-2048.
83. Keefer CL, Baldassare H, Keyston B et al. 2001 Generation of dwarf goat (Capra hircus) following nuclear transfer. Biology of Reproduction 64, 849-856.
84. Khosla S, Dean W, Brown D et al. 2001 Culture of mouse embryos affects fetal development and the expression of imprinted genes. Biology of Reproduction 64, 918-926.
85. Kimura H, Tada M, Nakatsuji N, Tada T 2004 Histone code modifications on pluripotential nuclei of reprogrammed somatic cells. Molecular and Cellular Biology 24, 5710-5720.
86. Ko MSH 2005 Molecular biology of preimplantation embryos: primer for philosophical discussion. Reproductive BioMedicine Online 10 (Suppl. 1), 80-87.
87. Kohn MJ, Bronson RT, Harlow et al. 2003 Dp1 required for extra-embryonic development. Development 130, 1295-1305.
88. Kono T, Obaya Y, Wu Q 2004 Birth of a parthenogenetic mouse that can develop to adulthood. Nature 428, 860-864.
89. Kratzer PG, Chapman VM, Lambert H et al. 1983 Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice. Cell 33, 37-42.
90. Kurimoto K, Yabuta Y, Ohinata Y et al. 2006 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis. Nucleic Acids Research 34 (5):e42 [e-pub ahead of print 17 March 2006; doi: 10.1093/nar/gk1050].
91. Lacham-Kaplan O, Daniels R, Trounson A 2001 Fertilization of mouse oocytes using somatic cells as male germ cells. Reproductive BioMedicine Online 2, 203-209.
92. Lagasse E, Connors H, Al-Dhalim M et al. 2000 Purified haematopoietic stem cells can differentiate into hepatocytes in vitro. Nature Medicine 6, 1229-1234.
93. Lane N, Dean W, Erhardt S et al. 2003 Resistance of IAPs to methylation reprogramming may provide a mechanism for epigenetic inheritance in the mouse. Genesis 35, 88-93.
94. Lavoir M-C, Weier J, Conaghan J, Pedersen R 2005 Poor development of human nuclear transfer embryos using failed fertilized oocytes. Reproductive BioMedicine Online 11, 740-744.
95. Leese HJ 1995 Metabolic control during preimplantation mammalian development. Human Reproduction Update 1, 63-72.
96. Lyon MF 1961 Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature 190, 372-373.
97. Mager J, Montgomery ND, de Villena FP-M, Magnuson T 2003 Genome imprinting regulated by the mouse polycomb group protein Eed. Nature Genetics 33, 502-507.
98. Maher ER, Brueton LA, Bowdin SC et al. 2000. Beckwith-Wiedemann syndrome and assisted reproduction technology. Journal of Medical Genetics 40, 62-64.
99. Mak W, Baxter J, Silva J et al. 2002 Mitotically stable association of polycomb proteins eed and enx1 with the inactive chromosome in trophoblast stem cells. Current Biology 12, 1016-1020.
100. Mayne CS, McEvoy J 1993 In vitro fertilized embryos: implications for the dairy herd. The Veterinary Annual 33, 1-4.
101. Meissner A, Jaenisch R 2006 Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx-2-deficient blastocysts. Nature 439, 212-215.
102. Ménard C, Hagège AA, Agbulut O et al. 2005 Transplantation of cardiac-committed mouse embryonic stem cells to infarcted sheep myocardium: a preclinical study. Lancet 366, 1005-1012.
103. Milhem M, Mahmud N, Lavelle D et al. 2004 Modification of haemopoietic stem cell fate by 5-aza2′deoxycytidine and trichstatin A. Blood 103, 4102-4110.
104. Nagy ZP 2004 Haploidization to produce human embryos: a new frontier in micromanipulation. Reproductive BioMedicine Online 8, 492-495.
105. + cells at a divergent point of endothelial and haemopoietic lineages. Development 125, 1747-1757.
106. Niwa H, Toyooka Y, Shimosato D et al. 2005 Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell 123, 917-929.
107. Niwa H, Miyazaki J, Smith AG 2000 Quantitative expression of Oct3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics 24, 372-376,
108. Ogunuki N, Inoue K, Yamomoto Y et al. 2002 Early death of mice cloned from somatic cells. Nature Genetics 30, 253-254.
109. Ohinata Y, Payer B, O-Carroll D et al. 2005 Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436, 207-213.
110. Ohsugi M, Hwang S-Y, Butz S et al. 1996 Expression and cell membrane localization of catenins during mouse preimplantation development. Developmental Biology 206, 391-402.
111. Olbrich H, Häffner K, Kispert A et al. 2002 Mutations in DNAH5 cause primary ciliary dyskinesis and randomization of left-right asymmetry. Nature Genetics 30, 143-144.
112. -/low/negative haemopoietic stem cell. Science 273, 242-245.
113. Palermo GD, Takeuchi T, Rosenwaks Z 2002 Oocyte-induced haploidization. Reproductive Biomedicine Online 4, 237-242.
114. Piko L, Clegg KB 1982 Quantitative changes in the total RNA, total poly(A) and ribosomes in early mouse embryos. Developmental Biology 89, 362-378.
115. Pinto-Correia C, Long C, Chang T, Robl JM 1995 Factors involved in nuclear reprogramming during early development in the rabbit. Molecular and Reproductive Development 40, 292-304.
116. Piotrowska-Nitsche K, Perea-Gomez A, Haraguchi S, Zernicke-Goetz M 2006 Four-cell mouse blastomeres have different developmental properties. Development, in press.
117. Plusa B, Frankenberg S, Chalmers A et al. 2005 Down-regulation of Par 3 and aPKC function directs cells towards the ICM in the preimplantation mouse embryo. Journal of Cell Science 118, 505-515.
118. Prather RS, Sims MM, Robl JM et al. 1987 Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biology of Reproduction 41, 123-132.
119. Purandare SM, Ware SM, Kwan KM et al. 2002 A complex syndrome of left-right axis, central nervous system and axial skeletal defects in ZIC3 mutant mice. Development 129, 2293-2302.
120. Qui J 2006 Unfinished symphony. Nature 441, 143-145.
121. Ramchandani S, Bhattacharya SK, Cervoni N, Szyf M 1999 DNA methylation is a reversible biological signal. Proceedings of the National Academies of Science, Washington 96, 6107-6112.
122. Reik W, Walter J 2001 Evolution of imprinting mechanisms: the battle of the sexes begins in the zygote. Nature Genetics 27, 255-256.
123. Reik W, Römer I, Barton SC 1993 Adult phenotype in the mouse can be affected by epigenetic events in the early embryo. Development 119, 933-942.
124. Rossant J 2001 Stem cells from the mammalian blastocyst. Stem Cells 19, 477-482.
125. Rossant J, Papaioannou VE 1997 The biology of embryogenesis. In: Sherman M (ed.) Concepts in Mammalian Embryogenesis. MTP Press, Cambridge, Massachusetts.
126. Rossant J, Chazoud C, Yamanaka Y 2003 Lineage allocation and asymmetries in the early mouse embryo. Philosophical Transactions of the Royal Society, London. B. Biological Sciences 358, 1341-1348.
127. Sado T, Okano M, Li E, Sasaki H 2004 De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation. Development 131, 975-982.
128. Sagata N 1997 What does Mos do in oocytes and comatic cells? Bioessays 19, 13-21.
129. Saitou M, Barton SC, Surani MA 2002 A molecular programme for the specification of germ cell fate in mice. Nature 418, 293-300.
130. Sandovici I, Naumoa AK, Leppert M et al. 2004 A longitudinal study on the X inactivation ratio in human females. Human Genetics 115, 387-392.
131. Santos F, Hendrich B, Reik W, Dean W 2001 Dynamic reprogramming on DNA methylation in the early mouse embryo. Developmental Biology 241 172-182.
132. Sathananthan H, Menezes H, Gunasheela G et al. 2003 Mechanisms of human blastocyst hatching in vitro. Reproductive BioMedicine Online 7, 228-234.
133. Saunders PTK, Pathirana S, Maguire SM et al. 2000 Mouse staufen genes are expressed in germ cells during oogenesis and spermatogenesis. Molecular Human Reproduction 6, 983-992.
134. Schlaeger TM, Hanna KA, Mikkola CG et al. 2005 Tie2Cre-mediated gene ablation defines the stem cell-leukemia gene (SCL/tal1)-dependent window during haemopoietic stem-cell development. Blood 105, 3871-3874.
135. Simonsson S, Gurdon J 2004 DNA methylation is necessary for the epigenetic reprogramming of somatic cell nuclei. Nature Cell Biology B, 984-990.
136. Singer-Sam J, Chapman V, Lebon JM, Riggs AD 1992 Parental imprinting studies by allele-specific primer extension after PCR paternal X chromosome-linked genes are transcribed prior to preferential paternal X chromosome inactivation. Proceedings of the National Academy of Sciences, Washington 89, 10469-10473.
137. Sollars V, Lu X, Xiao L et al. 2003 Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. Nature Genetics 33, 70-74.
138. Sönnischen B, Koski LB, Walsh A et al. 2005 Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature 434, 462-469
139. Stadfield M, Graf T 2004 Assessing the role of haemopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing. Development 132, 203-213.
140. Stojkovic M, Stoljovic P, Leary C et al. 2005 Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes. Reproductive BioMedicine Online 11, 226-231.
141. Strelchenko N, Kukharenko V, Shkumatov A et al. 2006 Reprogramming of human somatic cells by embryonic stem cell cytoplast. Reproductive BioMedicine Online 12, 107-111.
142. Strong C 2005 The ethics of human cloning. Reproductive BioMedicine Online 10 (Suppl.) 1, 45-49.
143. Surani MAH 2002 Immaculate nonconception. Nature 416, 491-493.
144. Surani MAH, Allen ND, Barton SC et al. 1990 Developmental consequences of imprinting of paternal chromosomes by DNA methylation. Philosophical Transactions of the Royal Society, London (Biology) 326, 313-327.
145. Szeto IY, Barton SC, Keveme EB, Surani AM 2004 Analysis of imprinted murine Peg3 locus in transgenic mice. Mammalian Genome 15, 284-295
146. Takahashi K, Yamanaka S 2006 Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
147. Takaoka K, Yamamoto M, Shiratori H et al. 2006 The mouse embryo autonomously acquires anterior-posterior polarity at implantation. Developmental Cell 10, 451-459.
148. Takeuchi T, Palermo G 2004 Clinical prospects of nuclear transfer and somatic cell haploidization: implications of cloning technique for reproductive medicine. Reproductive BioMedicine Online 8, 509-515.
149. Tang F, Hajkova P, Barton SC et al. 2006 MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Research 24, 34.
150. Tarkowski AK, Balakier H 1980 Nucleo-cytoplasmic interactions in cell hybrids between mouse oocytes, blastomeres and somatic cells. Journal of Embryology and Experimental Morphology 55, 319-330.
151. Tesarik J, Nagy ZP, Sousa M et al. 2001 Fertilizable oocytes reconstructed from patient's patient's somatic cell nuclei and donor ooplasts. Reproductive BioMedicine Online 2, 160-164.
152. Thornton JM 2001 Protein structures, inherited mutations and disease. In: Abstract PS02, Eleventh International Congress of Human Genetics, Vienna.
153. Tolkunova E, Cavaleri F, Eckardt S et al. 2006 The caudal-related protein Cdx2 promotes trophoblast differentiation on mouse ES cells. Stem Cells 24, 139-144.
154. Unwin RD, Smith DL, Blinco D et al. 2006 Quantitative proteonomics reveals posttranslational control as a regulatory factor in primary hemopoietic stem cells. Blood 107, 4687-4694.
155. Van Blerkom J, Barton SC, Johnson MH 1976 Molecular differentiation in the preimplantation mouse embryo. Nature 259, 319-321.
156. Verdel A, Jia S, Gerber S et al. 2004 RNA-mediated targeting of heterochromatin by the RITS complex. Science 303, 672-676.
157. Vinot S, Le Tran, Maro B, Louvet-Vallé S 2004 Two PAR6 proteins become asymmetrically localized during establishment of polarity in mouse oocytes. Current Biology 14, 520-525.
158. Waddington CH 1943 The epigenotype. Endeavour 1, 18-20.
159. Wang X, Willembring H, Akkari Y et al. 2003 Cell fusion is the principle source of bone-marrow derived hepatocytes. Nature 422, 897-901.
160. Weismann I 2005 Stem cell research. Journal of the American Medical Association 294, 1359-1360.
161. Wiley LM, Wu J-X, Harari I, Adamson ED 1992 Epidermal growth factor receptor mRNA and protein increase after the four-cell preimplantation stage in murine development. Developmental Biology 149, 247-260.
162. Wilmut I, Schmeike AE, McWhirter J et al. 1997 Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810-813.
163. Wolf SF, Migeon BR 1985 Clusters of CpG dinucleotides implicated by miclease hypersensitivity as control elements of housekeeping genes. Nature 314,467-469.
164. Wong PMC, Chung SW, Chui DHK, Eaves CJ 1986 Properties of the earliest clonogenic haematopoietic precursors to appear in the developing murine yolk sac. Proceedings of the National Academy of Sciences USA 83, 3851-3854.
165. Woodbury D, Reynolds K, Black IB 2002 Adult bone marrow stromal cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. Journal of Neuroscience Research 69, 908-917.
166. Wu L, de Bruin A, Saavedra HI et al. 2003 Extra-embryonic function of Rb is essential for embryonic development and viability. Nature 421, 942-947.
167. Wutz A, Jaenisch R 2000 A shift from reversible to irreversible inactivation during ES cell differentiation. Molecular Cell 5, 695-705.
168. Wutz A, Smzka OW, Barlow DP 1998 Making sense of imprinting in the mouse, p. 251-263. In: Chadwick DJ, Cardew G (eds) Epigenetics. Wiley, Chichester, UK.
169. Xue L, Chen X, Chang Y, Bieker JJ 2004 Regulatory elements in the EKLF gene that direct erythroid cell-specific expression during mammalian development. Blood 103, 4078-4083.
170. Young LE, Fernandes K, McEvoy TG et al. 2001 Sheep embryo culture. Nature Genetics 27, 153-154.
171. Zavos PM 2003 Human reproductive cloning: the time is near. Reproductive BioMedicine Online 6, 397-398.
172. Zernicke-Goetz M 2003 Determining the first cleavage of the mouse oocyte. Reproductive BioMedicine Online 6, 160-163.