Role of loop bundle hydrogen bonds in the maturation and activity of (Pro)caspase-3

Biochemistry

Volumn 45, Issue 44, 2006, Pages 13249-13263

  Feeney, Brett ^a   Pop, Cristina ^a,b   Swartz, Paul ^a   Mattos, Carla ^a   Clark, A Clay ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b BURNHAM INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; CRYSTAL STRUCTURE; FLUORESCENCE; HYDROGEN BONDS; MOLECULAR STRUCTURE; X RAY CRYSTALLOGRAPHY;

ACTIVE SITE LOOPS; CASPASE; LOOP BUNDLE; PROCASPASE;

ENZYMES;

CASPASE; PROCASPASE 3; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYMOLOGY; HYDROGEN BOND; IMMUNOBLOTTING; MUTATIONAL ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CASPASE 3; CRYSTALLOGRAPHY; DNA PRIMERS; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE;

EID: 33750690511     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0611964     Document Type: Article

References (32)

1. Cain, K., Brown, D. G., Langlais, C., and Cohen, G. M. (1999) Caspase activation involves the formation of the aposome, a large (∼700 kDa) caspase-activating complex, J. Biol. Chem. 274, 22686-22692.
2. Pop, C., Chen, Y.-R., Smith, B., Bose, K., Bobay, B., Tripathy, A., Franzen, S., and Clark, A. C. (2001) Removal of the pro-domain does not affect the conformation of the procaspase-3 dimer, Biochemistry 40, 14224-14235.
3. Boatright, K. M., Renatus, M. Scott, F. L., Sperandio, S., Shin, H., Pedersen, I. M., Ricci, J.-E., Edris, W. A., Sutherlin, D. P., Green, D. R., and Salvesen, G. (2003) A unified model for apical caspase activation, Mol. Cell 11, 529-541.
4. Donepudi, M., Sweeney, A. M., Briande, C., and Grutter, M. G. (2003) Insights into the regulatory mechanism lor caspase-8 activation, Mol. Cell 11, 543-549.
5. Zou, H., Li, Y., Liu, X., and Wang, X. (1999) An apaF-1-cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9, J. Biol. Chem. 274, 11549-11556.
6. Medema, J. P., Scaffidi, C., Kischkel, F. C., Shevchenko, A., Mann, M., Krammer, P. H., and Peter, M. E. (1997) FLICE is activated by association with the CD95 death-inducing signaling complex (DISC). EMBO J. 16, 2794-2804.
7. Stennicke, H. R., Deveraux, Q. L., Humke, E. W., Reed, J. C., Dixil, V. M., and Salvesen, G. S. (1999) Caspase-9 can he activated without proteolytic processing, J. Biol. Chem. 274, 8359-8362.
8. Srinivasula, S. M., Hegde, R., Saleh, A., Datta, P., Shiozaki, E., Chai, J., Lee, R.-A., Robbins, P. D., Fernandes-Alnemri, T., Shi, Y., and Alnemri, E. S. (2001) A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis. Nature 410, 112-116.
9. Bose, K., Pop, C., Feeney, B., and Clark, A. C. (2003) An uncleavable procaspase-3 mutant has a lower catalytic efficiency but an active site similar to that of mature caspase-3, Biochemistry 42, 12298-12310.
10. Stennicke, H. R., Jurgensmeier, J. M., Shin. H., Deveraux, Q., Wolf, B. B., Yang, X., Zhou, Q., Ellerby, M., Ellerby, L. M., Bredesen, D., Green, D. R., Reed, J. C., Froelich, C. J., and Salvesen, G. S. (1998) Pro-caspase-3 is a major physiologic target of caspase-8, J. Biol. Chem. 273, 27084-27090.
11. Mittl, P. R. E., DiMarco, S., Krebs, J. F., Bai, X., Karanewsky, D. S., Priestle, J. P., Tomaselli, K. J., and Grutter, M. G. (1997) Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-asp-val-ala-asp fluoromethyl ketone, J. Biol. Chem. 272, 6539-6547.
12. Rotonda, J., Nicholson, D. W., Fazil, K. M., Gallant, M., Gareau, Y., Labelle, M., Peterson, E. P., Rasper, D. M., Ruel, R., Vaillancourt, J. P., Thornberry, N. A., and Becker, J. W. (1996) The three-dimensional structure of apopain/CPP32. a key mediator of apoptosis. Nat. Struct. Biol. 3, 619-625.
13. Chai, J., Shiozaki, E., Srinivasula, S. M., Wu, Q., Dataa, P., Alnemri, E. S., and Shi, Y. (2001) Structural basis of caspase-7 inhibition by XIAP, Cell 104, 769-780.
14. Blanchard, H., Kodandapani, L., Mittl, P. R. E., Di Marco, S., Krebs, J. F., Wu, J. C., Tomaselli, K. J., and Grutter, M. G. (1999) The three-dimensional structure of caspase-8: An initiator enzyme in apoptosis, Structure 7, 1125-1133.
15. Wilson, K. P., Black, J.-A. F., Thomson, J. A., Kim, E. E., Griffith, J. P., Navia, M. A., Murcko, M. A., Chambers, S. P., Aldape, R. A., Raybuck, S. A., and Livingsion, D. J. (1994) Structure and mechanism of interleukin-1β converting enzyme. Nature 370, 270-275.
16. Schweizer, A., Briande, C., and Grutter, M. G. (2003) Crystal structure of caspase-2, apical initiator of the intrinsic apopiotic pathway, J. Biol. Chem. 278, 42441-42447.
17. Chai, J., Wu, Q., Shiozaki, E., Srinivasula, S. M., Alnemri, E. S., und Shi, Y. (2001) Crystal structure of a procaspase-7 zymogen: Mechanisms of activation and substrate binding, Cell 107, 399-407.
18. Ried, S. J., Fuentes-Prior, P., Renatus, M., Kairies, N., Krapp, S., Huber, R., Salvesen, G. S., and Bode, W. (2001) Structural basis for the activation of human procaspase-7, Proc. Natl. Acad. Sci. U.S.A. 98, 14790-14795.
19. Fuentes-Prior, P., and Salvesen, G. S. (2004) The protein structures that shape caspase activity, specificity activation and inhibition, Biochem. J. 384, 201-232.
20. Pop, C., Feeney, B., Tripathy, A., and Clark, A. C. (2003) Mutations in the procaspase-3 dimer interface affect the activity of the zymogen, Biochemistry 42, 12311-12320.
21. Feeney, B., Pop, C., Tripathy, A., and Clark, A. C. (2004) Ionic interactions near loop L4 are important for maintaining the active site environment and the dimer stability of (pro)caspase-3, Biochem. J. 384, 515-525.
22. Stennicke, H. R., and Salvesen, G. S. (1999) Caspases: Preparation and characterization, Methods 17, 313-319.
23. Royer, C. A., Mann, C. J., and Matthews, C. R. (1993) Resolution of the fluorescence equilibrium unfolding profile of trp apore-pressor using single tryptophan mutants, Protein Sci. 2, 1844-1852.
24. Brunger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Panni, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Crystallography and NMK system: A new software suite for macromolecular structure determination, Acta Crystallogr. D54, 905-921.
25. Jones, T. A., Zou, J. Y., Cowan, S. W., and Kjeldgaard, M. (1991) Improved methods for building protein models in electron density maps and the location of errors in these models, Acta Crystallogr. A47, 110-119.
26. Bose, K., and Clark, A. C. (2005) pH effects on the stability and dimerization of procaspase-3, Protein Sci. 14, 24-36.
27. Feeney, B., and Clark, A. C. (2005) Reassembly of active caspase-3 is facilitated by the propeptide, J. Biol. Chem. 280, 39772-39785.
28. Ni, C.-Z., Li, C., Wu, J. C., Spada, A. P., and Ely, K. R. (2003) Conformational restrictions in the active site of unliganded human caspase-3, J. Mol. Recognit. 16, 121-124.
29. Becker, J., Rotonda, J., Soisson, S. M., Aspiotis, K., Bayly, C., Francoeur, S., Gallant, M., Garcia-Calvo, M., Giroux, A., Grimm, E., Han, Y., McKay, D., Nicholson, D. W., Peterson, E., Renaud, J., Roy, S., Thornberry, N., and Zamboni, R. (2004) Reducing the peptidyl features of caspase-3 inhibitors: A structural analysis, J. Med. Chem. 47, 2466-2474.
30. Erlanson, D. A., Lam, J. W., Wiesmann, C., Luong, T. N., Simmons, R. L., DeLano, W. L., Choong, I. C., Burdett, M. T., Flanagan, W. M., Lee, D., Gordon, E. M., and O'Brien, T. (2003) In situ assembly of enzyme inhibitors using extended tethering, Nat. Biotechnol. 21, 308-314.
31. Lee, D., Long, S. A., Adams, J. M., Chan, G., Vaidya, K. S., Francis, T. A., Kikly, K., Winkler, J. D., Sung, C.-M., Debouck, C. Richardson, S., Levy, M. A., DeWolf, W. E., Jr., Keller, P. M., Tomaszek, T., Head, M. S., Ryan, D. M., Haltiwanger, R. C., Liang, P.-H., Janson, C. A., McDevitt, P. J., Johanson, K., Concha, N. O., Chan, W., Abdel-Meguid, S. S., Badger, A. M. Lark, M. W., Nadeau, D. P., Suva, L. J. Gowen, M., and Nuttall, M. E. (2000) Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality, J. Biol. Chem. 275, 16007-16014.
32. Riedl, S. J., Renatus, M., Schwarzenbacher, R., Zhou, Q., Sun, C., Fesik, S. W., Liddington, R. C., and Salvesen, G. S. (2001) Structural basis for the inhibition of caspase-3 by XIAP, Cell 104, 791-800.