Electrotransfer into skeletal muscle for protein expression

Current Gene Therapy

Volumn 6, Issue 5, 2006, Pages 561-578

  Trollet, C ^a,b,c,d   Bloquel, C ^a   Scherman, D ^a,b,c,d   Bigey, Pascal ^a,b,c,d  

^a INSERM   (France)

^b Centre National de la Recherche Scientifique   (France)

^c UNIVERSITÉ PARIS DESCARTES   (France)

^d PSL RESEARCH UNIVERSITY   (France)

Author keywords

Electroporation; Gene therapy

Indexed keywords

1 ALKYL 2 ACETYLGLYCEROPHOSPHOCHOLINE ESTERASE; ADENOVIRUS VECTOR; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 4; CALCITONIN GENE RELATED PEPTIDE; CARDIOTROPHIN 1; CISPLATIN; CYTOKINE; ECDYSONE; ERYTHROPOIETIN; GROWTH HORMONE RELEASING FACTOR; INTERFERON; INTERLEUKIN 1 RECEPTOR BLOCKING AGENT; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 18; INTERLEUKIN 4; KERATINOCYTE GROWTH FACTOR RECEPTOR; LEPTIN; MIFEPRISTONE; PLASMID DNA; PROINSULIN; PROOPIOMELANOCORTIN; RAPAMYCIN; RAPSYN; SCATTER FACTOR; SHORT HAIRPIN RNA; SOMATOMEDIN C; TETRACYCLINE; TUMOR NECROSIS FACTOR RECEPTOR; UNINDEXED DRUG;

ANALGESIA; ANTIANGIOGENIC ACTIVITY; ANTINEOPLASTIC ACTIVITY; ARTHRITIS; ATHEROSCLEROTIC PLAQUE; CANCER; CELL COMPARTMENTALIZATION; CELL MEMBRANE POTENTIAL; CELL PERMEABILIZATION; CLINICAL TRIAL; CONGESTIVE CARDIOMYOPATHY; CYTOTOXICITY; DIABETES MELLITUS; DNA DETERMINATION; DNA TRANSFER; DNA VECTOR; DRUG DISTRIBUTION; DRUG EFFICACY; DRUG HALF LIFE; DRUG MECHANISM; DRUG PROTEIN BINDING; DRUG SAFETY; ELECTROPORATION; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC CODE; HISTOPATHOLOGY; HUMAN; HYPERGLYCEMIA; IMMUNOPATHOLOGY; IMMUNOSTIMULATION; IN VITRO STUDY; IN VIVO STUDY; INSULIN DEFICIENCY; MELANOMA; METABOLIC DISORDER; MONOGENIC DISORDER; MOTOR NEUROPATHY; MUSCLE ATROPHY; MUSCLE DISEASE; MYASTHENIA GRAVIS; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; PAIN; PROTEIN EXPRESSION; PROTEIN SECRETION; RETREATMENT; REVIEW; SKELETAL MUSCLE; SKIN METASTASIS; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; DNA; ELECTROPORATION; GENE THERAPY; GENE TRANSFER TECHNIQUES; MODELS, ANIMAL; MUSCLE, SKELETAL; PLASMIDS; VACCINATION;

ANIMALIA;

EID: 33749506737     PISSN: 15665232     EISSN: None     Source Type: Journal    
DOI: 10.2174/156652306778520656     Document Type: Review

References (175)

1. Adachi, O., Nakano, A., Sato, O., Kawamoto, S., Tahara, H., Toyoda, N., Yamato, E., Matsumori, A., Tabayashi, K. and Miyazaki, J., (2002). Gene transfer of Fc-fusion cytokine by in vivo electroporation: application to gene therapy for viral myocarditis. Gene Ther., 9: 577-83.
2. Aihara, H. and Miyazaki, J., (1998). Gene transfer into muscle by electroporation in vivo. Nat. Biotechnol., 16: 867-70.
3. Andre, F. and Mir, L. M., (2004). DNA electrotransfer: its principles and an updated review of its therapeutic applications. Gene Ther., 11 Suppl 1: S33-42.
4. Arruda, V. R., Hagstrom, J. N., Deitch, J., Heiman-Patterson, T., Camire, R. M., Chu, K., Fields, P. A., Herzog, R. W., Couto, L. B., Larson, P. J. and High, K. A., (2001). Posttranslational modifications of recombinant myotube-synthesized human factor IX. Blood, 97: 130-8.
5. Ataka, K., Maruyama, H., Neichi, T., Miyazaki, J. and Gejyo, F., (2003). Effects of erythropoietin-gene electrotransfer in rats with adenine-induced renal failure. Am. J. Nephrol., 23: 315-23.
6. Babiuk, L. A., Pontarollo, R., Babiuk, S., Loehr, B. and van Drunen Littelvan den Hurk, S., (2003). Induction of immune responses by DNA vaccines in large animals. Vaccine, 21: 649-58.
7. Babiuk, S., Baca-Estrada, M. E., Foldvari, M., Middleton, D.M., Rabussay, D., Widera, G. and Babiuk, L. A., (2004). Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccins. J. Biotechnol, 110: 1-10.
8. Babiuk, S., Baca-Estrada, M. E., Foldvari, M., Storms, M., Rabussay, D., Widera, G. and Babiuk, L. A., (2002). Electroporation improves the efficacy of DNA vaccines in large animals. Vaccine, 20: 3399-3408.
9. Bachy, M., Boudet, F., Bureau, M., Girerd-Chambaz, Y., Wils, P., Scherman, D. and Meric, C., (2001). Electric pulses increase the immunogenicity of an influenza DNA vaccine injected intramuscularly in the mouse. Vaccine, 19: 1688-93.
10. Belehradek, J., Jr., Orlowski, S., Ramirez, L. H., Pron, G., Poddevin, B. and Mir, L. M., (1994). Electropermeabilization of cells in tissues assessed by the qualitative and quantitative electroloading of bleomycin. Biochim. Biophys. Acta, 1190: 155-63.
11. Bertoni, C., Jarrahian, S., Wheeler, T. M., Li, Y., Olivares, E. C., Calos, M. P. and Rando, T. A., (2006). Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integration. Proc. Natl. Acad. Sci. USA, 103: 419-24.
12. Bertrand, A., Ngo-Muller, V., Hentzen, D., Concordet, J. P., Daegelen, D. and Tuil, D., (2003). Muscle electrotransfer as a tool for studying muscle fiber-specific and nerve-dependent activity of promoters. Am. J. Physiol. Cell Physiol., 285: C1071-81.
13. Bettan, M., Darteil, R. and Scherman, D., (1999). Secreted human placental alkaline phosphatase as a reporter gene for in vivo gene transfer. Anal. Biochem., 271: 187-9.
14. Bettan, M., Emmanuel, F., Darteil, R., Caillaud, J. M., Soubrier, F., Delaere, P., Branelec, D., Mahfoudi, A., Duverger, N. and Scherman, D., (2000). High-level protein secretion into blood circulation after electric pulse-mediated gene transfer into skeletal muscle. Mol. Ther., 2: 204-10.
15. Bloquel, C., Bejjani, R., Bigey, P., Bedioui, F., Doat, M., BenEzra, D., Scherman, D. and Behar-Cohen, F., (2006). Plasmid electrotransfer of eye ciliary muscle: principles and therapeutic efficacy using hTNF-alpha soluble receptor in uveitis. FASEB J., 20: 389-91.
16. Bloquel, C., Bessis, N., Boissier, M. C., Scherman, D. and Bigey, P., (2004). Gene therapy of collagen-induced arthritis by electrotransfer of human tumor necrosis factor-alpha soluble receptor I variants. Hum. Gene Ther., 15: 189-201.
17. Bonnafous, P., Vernhes, M., Teissie, J. and Gabriel, B., (1999). The generation of reactive-oxygen species associated with long-lasting pulse-induced electropermeabilisation of mammalian cells is based on a non-destructive alteration of the plasma membrane. Biochim. Biophys. Acta, 1461: 123-34.
18. Bossard, C., Van den Berghe, L., Laurell, H., Castano, C., Cerutti, M., Prats, A. C. and Prats, H., (2004). Antiangiogenic properties of fibstatin, an extracellular FGF-2-binding polypeptide. Cancer Res., 64: 7507-12.
19. Bureau, M. F., Gehl, J., Deleuze, V., Mir, L. M. and Scherman, D., (2000). Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransfer. Biochim. Biophys. Acta, 1474:353-9.
20. Bureau, M. F., Naimi, S., Torero Ibad, R., Seguin, J., Georger, C., Arnould, E., Maton, L., Blanche, F., Delaere, P. and Scherman, D., (2004). Intramuscular plasmid DNA electrotransfer: biodistribution and degradation. Biochim. Biophys. Acta, 1676: 138-48.
21. Cappelletti, M., Zampaglione, I., Rizzuto, G., Ciliberto, G., La Monica, N. and Fattori, E., (2003). Gene electro-transfer improves transduction by modifying the fate of intramuscular DNA. J. Gene Med., 5: 324-32.
22. Celiker, M. Y., Wang, M., Atsidaftos, E., Liu, X., Liu, Y. E., Jiang, Y., Valderrama, E., Goldberg, I. D. and Shi, Y. E., (2001). Inhibition of Wilms' tumor growth by intramuscular administration of tissue inhibitor of metalloproteinases-4 plasmid DNA. OncoGene, 20: 4337-43.
23. Chen, Y., Zhang, T., Li, T., Han, W., Zhang, Y. and Ma, D., (2005). Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation. Hybridoma (Larchmt)., 24: 305-8.
24. Chuang, I. C., Jhao, C. M., Yang, C. H., Chang, H. C., Wang, C. W., Lu, C. Y., Chang, Y. J., Lin, S. H., Huang, P. L. and Yang, L. C., (2004). Intramuscular electroporation with the pro-opiomelanocortin gene in rat adjuvant arthritis. Arthritis Res. Ther., 6: R7-R14.
25. Cichon, T., Jamrozy, L., Glogowska, J., Missol-Kolka, E. and Szala, S., (2002). Electrotransfer of gene encoding endostatin into normal and neoplastic mouse tissues: inhibition of primary tumor growth and metastatic spread. Cancer Gene Ther., 9: 771-7.
26. Cleasby, M. E., Davey, J. R., Reinten, T. A., Graham, M. W., James, D. E., Kraegen, E. W. and Cooney, G. J., (2005). Acute bidirectional manipulation of muscle glucose uptake by in vivo electrotransfer of constructs targeting glucose transporter genes. Diabetes, 54: 2702-11.
27. Croze, F. and Prud'homme, G. J., (2003). Gene therapy of streptozotocin-induced diabetes by intramuscular delivery of modified preproinsulin genes. J. Gene Med. 5: 425-37.
28. Dalle, B., Henri, A., Rouyer-Fessard, P., Bettan, M., Scherman, D., Beuzard, Y. and Payen, E., (2001). Dimeric erythropoietin fusion protein with enhanced erythropoietic activity in vitro and in vivo. Blood, 97: 3776-82.
29. Davis, H. L., Whalen, R. G. and Demeneix, B. A., (1993). Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. Hum. Gene Ther., 4: 151-9.
30. de Gennes, P. G., (1999). Passive entry of a DNA molecule into a small pore. Proc. Natl. Acad. Sci. USA, 96: 7262-4.
31. Draghia-Akli, R., Cummings, K. K., Khan, A. S., Brown, P. A. and Carpenter, R. H., (2003). Effects of plasmid-mediated growth hormone releasing hormone supplementation in young, healthy Beagle dogs. J. Anim. Sci., 81: 2301-10.
32. Draghia-Akli, R., Ellis, K. M., Hill, L. A., Malone, P. B. and Fiorotto, M. L., (2003). High-efficiency growth hormone-releasing hormone plasmid vector administration into skeletal muscle mediated by electroporation in pigs. FASEB J., 17: 526-8.
33. Draghia-Akli, R. and Fiorotto, M. L., (2004). A new plasmid-mediated approach to supplement somatotropin production in pigs. J. Anim. Sci., 82 E-Suppl: E264-269.
34. Draghia-Akli, R., Fiorotto, M. L., Hill, L. A,, Malone, P. B., Deaver, D. R. and Schwartz, R. J., (1999). Myogenic expression of an injectable protease-resistant growth hormone-releasing hormone augments long-term growth in pigs. Nat. Biotechnol., 17: 1179-83.
35. Durieux, A. C., Bonnefoy, R., Busso, T. and Freyssenet, D., (2004). In vivo gene electrotransfer into skeletal muscle: effects of plasmid DNA on the occurrence and extent of muscle damage. J. Gene Med., 6: 809-16.
36. Fattori, E., Cappelletti, M., Zampaglione, I., Mennuni, C., Calvaruso, F., Arcuri, M., Rizzuto, G., Costa, P., Perretta, G., Ciliberto, G. and La Monica, N., (2005). Gene electro-transfer of an improved erythropoietin plasmid in mice and non-human primates. J Gene Med., 7: 228-36.
37. Faurie, C., Phez, E., Golzio, M., Vossen, C., Lesbordes, J. C., Delteil, C., Teissie, J. and Rols, M. P., (2004). Effect of electric field vectoriality on electrically mediated gene delivery in mammalian cells. Biochim. Biophys. Acta, 1665: 92-100.
38. Favre, D., Cherel, Y., Provost, N., Blouin, V., Ferry, N., Moullier, P. and Salvetti, A., (2000). Hyaluronidase enhances recombinant adeno-associated virus (rAAV)-mediated gene transfer in the rat skeletal muscle. Gene Ther., 7: 1417-20.
39. Ferrer, A., Foster, H., Wells, K. E., Dickson, G. and Wells, D. J., (2004). Long-term expression of full-length human dystrophin in transgenic mdx mice expressing internally deleted human dystrophins. Gene Ther., 11: 884-93.
40. Fewell, J. G., MacLaughlin, F., Mehta, V., Gondo, M., Nicol, F., Wilson, E. and Smith, L. C., (2001). Gene therapy for the treatment of hemophilia B using PINC-formulated plasmid delivered to muscle with electroporation. Mol. Ther., 3: 574-83.
41. Fussenegger, M., (2001). The impact of mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies. Biotechnol. Prog., 17: 1-51.
42. Gehl, J. and Mir, L. M., (1999). Determination of optimal parameters for in vivo gene transfer by electroporation, using a rapid in vivo test for cell permeabilization. Biochem. Biophys. Res. Commun., 261: 377-80.
43. Gehl, J., Sorensen, T. H., Nielsen, K., Raskmark, P., Nielsen, S. L., Skovsgaard, T. and Mir, L. M., (1999). In vivo electroporation of skeletal muscle: threshold, efficacy and relation to electric field distribution. Biochim. Biophys. Acta, 1428: 233-40.
44. Goldspink, G., (2003). Skeletal muscle as an artificial endocrine tissue. Best Pract. Res. Clin. Endocrinol. Metab., 17: 211-22.
45. Gollins, H., McMahon, J., Wells, K. E. and Wells, D. J., (2003). High-efficiency plasmid gene transfer into dystrophic muscle. Gene Ther., 10: 504-12.
46. Golzio, M., Rols, M. P. and Teissie, J., (2004). In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression. Methods, 33: 126-35.
47. Golzio, M., Teissie, J. and Rols, M. P., (2002). Direct visualization at the single-cell level of electrically mediated gene delivery. Proc. Natl. Acad. Sci. USA, 99: 1292-7.
48. Gossen, M. and Bujard, H., (1992). Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA, 89: 5547-51.
49. Gothelf, A., Mir, L. M. and Gehl, J., (2003). Electrochemotherapy: results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat Rev., 29: 371-87.
50. Gould, D. J., Bright, C. and Chernajovsky, Y., (2004). Inhibition of established collagen-induced arthritis with a tumour necrosis factor-alpha inhibitor expressed from a self-contained doxycycline regulated plasmid. Arthritis Res. Ther., 6: R103-13.
51. Goverdhana, S., Puntel, M., Xiong, W., Zirger, J. M., Barcia, C., Curtin, J. F., Soffer, E. B., Mondkar, S., King, G. D., Hu, J., Sciascia, S. A., Candolfi, M., Greengold, D. S., Lowenstein, P. R. and Castro, M. G., (2005). Regulatable gene expression systems for gene therapy applications: progress and future challenges. Mol. Ther., 12: 189-211.
52. Gronevik, E., von Steyern, F.V., Kalhovde, J.M., Tjelle, T.E. and Mathiesen, I., (2005). Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle. J. Gene Med., 7: 218-227.
53. Hartikka, J., Sukhu, L., Buchner, C., Hazard, D., Bozoukova, V., Margalith, M., Nishioka, W. K., Wheeler, C. J., Manthorp, M. and Sawdey, M., (2001). Electroporation-facilitated delivery of plasmid DNA in skeletal muscle: plasmid dependence of muscle damage and effect of poloxamer 188. Mol. Ther., 4: 407-15.
54. Hase, M., Tanaka, M., Yokota, M. and Yamada, Y., (2002). Reduction in the extent of atherosclerosis in apolipoprotein E-deficient mice induced by electroporation-mediated transfer of the human plasma platelet-activating factor acetylhydrolase gene into skeletal muscle. Prostaglandins Other Lipid Mediat., 70: 107-18.
55. Heller, L. C., Ingram, S. F., Lucas, M. L., Gilbert, R. A. and Heller, R., (2002). Effect of electrically mediated intratumor and intramuscular delivery of a plasmid encoding IFN alpha on visible B16 mouse melanomas. Technol. Cancer Res. Treat., 1: 205-9.
56. Heller, L. C., Ugen, K. and Heller, R., (2005). Electroporation for targeted gene transfer. Expert Opin. Drug Deliv., 2: 255-68.
57. Ho, S. H., Hahn, W., Lee, H. J., Kim, D. S., Jeong, J. G., Kim, S., Yu, S. S., Jeon, E. S. and Kim, J. M., (2004). Protection against collagen-induced arthritis by electrotransfer of an expression plasmid for the interleukin-4. Biochem. Biophys. Res. Commun., 321: 759-66.
58. Honigman, A., Zeira, E., Ohana, P., Abramovitz, R., Tavor, E., Bar, I., Zilberman, Y., Rabinovsky, R., Gazit, D., Joseph, A., Panet, A., Shai, E., Palmon, A., Laster, M. and Galun, E., (2001). Imaging transgene expression in live animals. Mol. Ther., 4: 239-49.
59. Horiki, M., Yamato, E., Noso, S., Ikegami, H., Ogihara, T. and Miyazaki, J., (2003). High-level expression of interleukin-4 following electroporation-mediated gene transfer accelerates Type 1 diabetes in NOD mice. J. Autoimmun., 20: 111-7.
60. Jeong, J. G., Kim, J. M., Ho, S. H., Hahn, W., Yu, S. S. and Kim, S., (2004). Electrotransfer of human IL-1Ra into skeletal muscles reduces the incidence of murine collagen-induced arthritis, J. Gene Med., 6: 1125-33.
61. Jiang, J., Yamato, E. and Miyazaki, J., (2003). Sustained expression of Fc-fusion cytokine following in vivo electroporation and mouse strain differences in expression levels. J., Biochem. (Tokyo), 133: 423-7.
62. Kawai, M., Bessho, K., Kaihara, S., Sonobe, J., Oda, K., Iizuka, T. and Maruyama, H., (2003). Ectopic bone formation by human bone morphogenetic protein-2 gone transfer to skeletal muscle using transcutaneous electroporation. Hum. Gene Ther., 14: 1547-56.
63. Kim, J. M., Ho, S. H., Hahn, W., Jeong, J. G., Park, E. J., Lee, H. J., Yu, S. S., Lee, C. S., Lee, Y. W. and Kim, S., (2003). Electro-gene therapy of collagen-induced arthritis by using an expression plasmid for the soluble p75 tumor necrosis factor receptor-Fe fusion protein. Gene Ther., 10: 1216-24.
64. Kirman, J. R. and Seder, R. A., (2003). DNA vaccination: the answer to stable, protective T-cell memory? Curr. Opin. Immunol., 15: 471-6.
65. Kishida, T., Asada, H., Gojo, S., Ohashi, S., Shin-Ya, M., Yasutomi, K., Terauchi, R., Takahashi, K. A., Kubo, T., Imanishi, J. and Mazda, O., (2004). Sequence-specific gene silencing in murine muscle induced by electroporation-mediated transfer of short interfering RNA. J. Gene Med., 6: 105-10.
66. Kishimoto, K. N., Watanabe, Y., Nakamura, H. and Kokubun, S., (2002). Ectopic bone formation by electroporatic transfer of bone morphogenetic protein-4 gene. Bone, 31: 340-7.
67. Klenchin, V. A., Sukharev, S. I., Serov, S. M., Chemomordik, L. V. and Chizmadzhev Yu, A., (1991). Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis. Biophys. J., 60: 804-11.
68. Komamura, K., Tatsumi, R., Miyazaki, J., Matsumoto, K., Yamato, E., Nakamura, T., Shimizu, Y., Nakatani, T., Kitamura, S., Tomoike, H., Kitakaze, M., Kangawa, K. and Miyatake, K., (2004). Treatment of dilated cardiomyopathy with electroporation of hepatocyte growth factor gene into skeletal muscle. Hypertension 44: 365-71.
69. Kon, O. L., Sivakumar, S., Teoh, K. L., Lok, S. H. and Long, Y. C., (1999). Naked plasmid-mediated gene transfer to skeletal muscle ameliorates diabetes mellitus. J. Gene Med., 1: 186-94.
70. Konig, S., Burkman, J., Fitzgerald, J., Mitchell, M., Su, L. and Stedman, H., (2002). Modular organization of phylogenetically conserved domains controlling developmental regulation of the human skeletal myosin heavy chain gene family. J. Biol. Chem., 277: 27593-605.
71. Konishi, Y., Stegmuller, J., Matsuda, T., Bonni, S. and Bonni, A., (2004). Cdhl-APC controls axonal growth and patterning in the mammalian brain. Science, 303: 1026-30.
72. Kreiss, P., Bettan, M., Crouzet, J. and Scherman, D., (1999). Erythropoietin secretion and physiological effect in mouse after intramuscular plasmid DNA electrotransfer. J. Gene Med., 1: 245-50.
73. Krieg A.M., (2002). CpG motifs in bacterial DNA and their immune effects. Annu. Rev. Immunol., 20: 709-60.
74. Krull, C. E., (2004). A primer on using in ovo electroporation to analyze gene function. Dev. Dyn., 229: 433-9.
75. Lamartina, S., Roscilli, G., Rinaudo, C. D., Sporeno, E., Silvi, L., Hillen, W., Bujard, H., Cortese, R., Ciliberto, G. and Toniatti, C., (2002). Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators. Hum. Gene Ther., 13: 199-210.
76. Lasne, F., Martin, L., de Ceaurriz, J., Larcher, T., Moullier, P. and Chenuaud, P., (2004). "Genetic Doping" with erythropoietin cDNA in primate muscle is detectable. Mol. Ther., 10: 409-10.
77. Ledwith, B. J., Manam, S., Troilo, P. J., Barnum, A. B., Pauley, C. J., Griffiths, T. G., 2nd, Harper, L. B., Beare, C. M., Bagdon, W. J. and Nichols, W. W., (2000). Plasmid DNA vaccines: investigation of integration into host cellular DNA following intramuscular injection in mice. Intervirology, 43: 258-72.
78. Ledwith, B. J., Manam, S., Troilo, P. J., Barnum, A. B., Pauley, C. J, Griffiths, T. G., 2nd, Harper, L. B., Schock, H. B., Zhang, H., Faris, J. E., Way, P. A., Beare, C. M., Bagdon, W. J. and Nichols, W. W., (2000). Plasmid DNA vaccines: assay for integration into host genomic DNA. Dev. Biol. (Basel), 104: 33-43.
79. Lee, A. H., Suh, Y. S., Sung, J. H., Yang, S. H. and Sung, Y. C., (1997). Comparison of various expression plasmids for the induction of immune response by DNA immunization. Mol. Cells, 7: 495-501.
80. Lee, S. C., Wu, C. J., Wu, P. Y., Huang, Y. L., Wu, C. W. and Tao, M. H., (2003). Inhibition of established subcutaneous and metastatic murine tumors by intramuscular electroporation of the interleukin-12 gene. J. Biomed Sci., 10: 73-86.
81. Leroy-Willig, A., Bureau, M. F., Scherman, D. and Carlier, P. G., (2005). In vivo NMR imaging evaluation of efficiency and toxicity of gene electrotransfer in rat muscle. Gene Ther., 12: 1434-43.
82. Lesbordes, J. C., Bordet, T., Haase, G., Castelnau-Ptakhine, L., Rouhani, S., Gilgenkrantz, H. and Kahn, A., (2002). In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice. Hum. Mol. Genet., 11: 1615-25.
83. Li, S., (2004). Electroporation gene therapy: new developments in vivo and in vitro. Curr. Gene Ther., 4: 309-16.
84. Li, S., Zhang, L., Torrero, M., Cannon, M. and Barret, R., (2005). Administration route- and immune cell activation-dependent tumor eradication by IL12 electransfer. Mol. Ther., 12: 942-9.
85. Li, S., Zhang, X., Xia, X., Zhou, L., Breau, R., Suen, J. and Hanna, E., (2001). Intramuscular electroporation delivery of IFN-alpha gene therapy for inhibition of tumor growth located at a distant site. Gene Ther., 8: 400-7.
86. Li, Z., Zhang, H., Fan, X., Zhang, Y., Huang, J., Liu, Q., Tjelle, T. E., Mathiesen, I., Kjeken, R. and Xiong, S., (2005). DNA electroporation prime and protein boost strategy enhances humoral immunity of tuberculosis DNA vaccines in mice and non-human primates. Vaccine.
87. Liu, F., Heston, S., Shollenberger, L. M., Sun, B., Mickle, M., Lovell, M. and Huang, L., (2006). Mechanism of in vivo DNA transport into cells by electroporation: electrophoresis across the plasma membrane may not be involved. J. Gene Med., 8: 353-61.
88. Long, Y. C., Jaichandran, S., Ho, L. P., Tien, S. L., Tan, S. Y. and Kon, O. L., (2005). FVIII gene delivery by muscle electroporation corrects murine hemophilia A. J. Gene Med, 7: 494-505.
89. Losen, M., Stassen, M. H., Martinez-Martinez, P., Machiels, B. M., Duimel, H., Frederik, P., Veldman, H., Wokke, J. H., Spaans, F., Vincent, A. and De Baets, M. H., (2005). Increased expression of rapsyn in muscles prevents acetylcholine receptor loss in experimental autoimmune myasthenia gravis. Brain, 128: 2327-37.
90. Lu. O. L.. Bou-Gharios. G. and Partridge. T. A.. (2003). Non-viral gene delivery in skeletal muscle: a protein factory. Gene Ther., 10: 131-42.
91. Lucas, M. L. and Heller, R., (2001). Immunomodulation by electrically enhanced delivery of plasmid DNA encoding IL-12 to murine skeletal muscle. Mol. Ther., 3:47-53.
92. Mallat, Z., Besnard, S., Duriez, M., Deleuze, V., Emmanuel, F., Bureau, M. F., Soubrier, F., Esposito, B., Duez, H., Fievet, C., Staels, B., Duverger, N., Scherman, D. and Tedgui, A., (1999). Protective role of interleukin-10 in atherosclerosis. Circ. Res., 85: e17-24.
93. Mallat, Z., Corbaz, A., Scoazec, A., Graber, P., Alouani, S., Esposito, B., Humbert, Y., Chvatchko, Y. and Tedgui, A., (2001).' Interleukin18/ interleukin-18 binding protein signaling modulates atherosclerotic lesion development and stability. Circ. Res., 89: E41-5.
94. Mallat, Z., Silvestre, J. S., Le Ricousse-Roussanne, S., Lecomte-Raclet, L., Corbaz, A., Clergue, M., Duriez, M., Barateau, V., Akira, S., Tedgui, A., Tobelem, G., Chvatchko, Y. and Levy, B. I., (2002). Interleukin18/interleukin-18 binding protein signaling modulates ischemia-induced neovascularization in mice hindlimb. Circ. Res., 91: 441-8.
95. Manam, S., Ledwith, B. J., Barnum, A. B., Troilo, P. J., Pauley, C. J., Harper, L. B., Griffiths, T. G., 2nd, Niu, Z., Denisova, L., Follmer, T. T., Pacchione, S. J., Wang, Z., Beare, C. M., Bagdon, W. J. and Nichols, W. W., (2000). Plasmid DNA vaccines: tissue distribution and effects of DNA sequence, adjuvants and delivery method on integration into host DNA. Intervirology, 43: 273-81.
96. Martel-Renoir, D., Trochon-Joseph, V., Galaup, A., Bouquet, C., Griscelli, F., Opolon, P., Opolon, D., Connault, E., Mir, L. and Perricaudet, M., (2003). Coelectrotransfer to skeletal muscle of three plasmids coding for antiangiogenic factors and regulatory factors of the tetracycline inducible system: tightly regulated expression, inhibition of transplanted tumor growth, and antimetastatic effect. Mol. Ther., 8: 425-33.
97. Martin, T., Parker, S. E., Hedstrom, R., Le, T., Hoffman, S. L., Norman, J., Hobart, P. and Lew, D., (1999). Plasmid DNA malaria vaccine: the potential for genomic integration after intramuscular injection. Hum. Gene Ther., 10: 759-68.
98. Martinenghi, S., Cusella De Angelis, G., Biressi, S., Amadio, S., Bifari, F., Roncarolo, M. G., Bordignon, C. and Falqui, L., (2002). Human insulin production and amelioration of diabetes in mice by electrotransfer-enhanced plasmid DNA gene transfer to the skeletal muscle. Gene Ther., 9: 1429-37.
99. Maruyama, H., Ataka, K., Gejyo, F., Higuchi, N., Ito, Y., Hirahara, H., Imazeki, L., Hirata, M., Ichikawa, F., Neichi, T., Kikuchi, H., Sugawa, M. and Miyazaki, J., (2001). Long-term production of erythropoietin after electroporation-mediated transfer of plasmid. DNA into the muscles of normal and uremic rats. Gene Ther., 8: 461-8.
100. Maruyama, H., Higuchi, M., Higuchi, N., Kameda, S., Saito, M., Sugawa, M.,Matsuzaki, J., Neichi, T., Yokoyama, S., Miyazaki, Y., Miyazaki, J. and Gejyo, F., (2004). Post-secretion neutralization of transgene derived effect: soluble erythropoietin receptor/IgG1fc expressed in liver neutralizes erythropoietin produced in muscle. J. Gene Med., 6: 228-37.
101. Maruyama, H., Sugawa, M., Moriguchi, Y., Imazeki, I, Ishikawa, Y., Ataka, K., Hasegawa, S., Ito, Y., Higuchi, N., Kazama, J. J., Gejyo, F. and Miyazaki, J. I., (2000). Continuous erythropoietin delivery by muscle targeted gene transfer using in vivo electroporation. Hum. Gene Ther., 11: 429-37.
102. Mathiesen, I., (1999). Electropermeabilization of skeletal muscle enhances gene transfer in vivo. Gene Ther., 6: 508-14.
103. Matsuda, T. and Cepko, C. L., (2004). Electroporation and RNA interference in the rodent retina in vivo and in vitro. Proc. Natl. Acad Sci. ' USA, 101: 16-22.
104. McMahon, J.M., Wells, K.E., Bamfo, J.E., Cartwright, M.A. and Wells, D.J., (1998). Inflammatory responses following direct injection of plasmid DNA into skeletal muscle. Gene Ther, 5: 1283-1290.
105. McMahon, J. M., Signori, E., Wells, K. E., Fazio, V. M. and Wells, D. J., (2001). Optimisation of electrotransfer of plasmid into skeletal muscle by pretreatment with hyaluronidase -- increased expression with reduced muscle damage. Gene Ther., 8: 1264-70.
106. Mei, W. H., Qian, G. Q., Zhang, X. Q., Zhang, P. and Lu, J., (2006). Sustained expression of Epstein-Barr virus episomal vector mediated factor VIII in vivo following muscle electroporation. Haemophilia, 12: 271-9.
107. Mennuni, C., Calvaruso, F., Zampaglione, I., Rizzuto, G., Rinaudo, D., Dammassa, E., Ciliberto, G., Fattori, E. and La Monica, N., (2002). Hyaluronidase increases electrogene transfer efficiency in skeletal muscle. Hum. Gene Ther., 13: 355-65.
108. Mir, L. M., Bureau, M. F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J. M., Delaere. P., Branellec. D., Schwartz. B. and Scherman. D., (1999). High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc. Natl. Acad Sci. USA, 96: 4262-7.
109. Mir, L. M., Bureau, M. F., Rangara, R., Schwartz, B. and Scherman, D., (1998). Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle. C. R. Acad. Sci, III., 321: 893-9.
110. Mir, L. M. and Orlowski, S., (1999). Mechanisms of electrochemotherapy. Adv. Drug Deliv. Rev., 35: 107-118.
111. Molnar, M. J., Gilbert, R., Lu, Y., Liu, A. B., Guo, A., Larochelle, N., Orlopp, K., Lochmuller, H., Petrof, B. J., Nalbantoglu, J. and Karpati, G., (2004). Factors influencing the efficacy, longevity, and safety of electroporation-assisted plasmid-based gene transfer into mouse muscles. Mol. Ther., 10: 447-55.
112. Murakami, T., Nishi, T., Kimura, E., Goto, T., Maeda, Y., Ushio, Y., Uchino, M. and Sunada, Y., (2003). Full-length dystrophin cDNA transfer into skeletal muscle of adult mdx mice by electroporation. Muscle Nerve 27: 237-41.
113. Nakano, A., Matsumori, A., Kawamoto, S., Tahara, H., Yamato, E., Sasayama, S. and Miyazaki, J. I., (2001). Cytokine gene therapy for myocarditis by in vivo electroporation. Hum. Gene Ther., 12: 1289-97.
114. Neumann, E., Schaefer-Ridder, M., Wang, Y. and Hofschneider, P. H., (1982). Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J., 1: 841-5.
115. Nichols, W. W., Ledwith, B. J., Manam S. V. and Troilo, P. J., (1995). Potential DNA vaccine integration into host cell genome. Ann. N. Y. Acad. Sci., 772: 30-9.
116. Nicol, F., Wong, M., MacLaughlin, F. C., Perrard, J., Wilson, E., Nordstrom, J. L. and Smith, L. C., (2002). Poly-L-glutamate, an anionic polymer, enhances transgene expression for plasmids delivered by intramuscular injection with in vivo electroporation. Gene Ther., 9:1351-8.
117. Nishikage, S., Koyama, H., Miyata, T., Ishii, S., Hamada, H. and Shigematsu, H., (2004). In vivo electroporation enhances plasmid-based gene transfer of basic fibroblast growth factor for the treatment of ischemic limb. J. Surg. Res., 120: 37-46.
118. Oikawa, Y., Shimada, A., Kasuga, A., Morimoto, J., Osaki, T., Tahara, H., Miyazaki, T., Tashiro, F., Yamato, E., Miyazaki, J. and Saruta, T., (2003). Systemic administration of IL-18 promotes diabetes development in young nonobese diabetic mice. J. Immunol., 171: 5865-75.
119. Otten, G., Schaefer, M., Doe, B., Liu, H., Srivastava, I., zur Megede, J., O'Hagan, D., Donnelly, J., Widera, G., Rabussay, D., Lewis, M. G., Barnett, S. and Ulmer, J. B., (2004). Enhancement of DNA vaccine potency in rhesus macaques by electroporation. Vaccine, 22: 2489-93.
120. Paturneau-Jouas, M., Parzy, E., Vidal, G., Bozin, P., Vilquin, J.-T., Wary, C., Carlier, P., Schwartz, K. and Leroy-Willig, A., (2001). Electroporation-mediated delivery of a magnetic resonance imaging contrast agent into muscle to visualize electrotransfer. Acta Myologica., 20: 174-178.
121. Payen, E., Bettan, M., Henri, A., Tomkiewitcz, E., Houque, A., Kuzniak, I., Zuber, J., Scherman, D. and Beuzard, Y., (2001). Oxygen tension and a pharmacological switch in the regulation of transgene expression for gene therapy. J. Gene Med., 3: 498-504.
122. Payen, E., Bettan, M., Rouyer-Fessard, P., Beuzard, Y. and Scherman, D., (2001). Improvement of mouse beta-thalassemia by electrotransfer of erythropoietin cDNA. Exp. Hematol., 29: 295-300.
123. Pekarik, V., Bourikas, D., Miglino, N., Joset, P., Preiswerk, S. and Stoeckli, E. T., (2003). Screening for gene function in chicken embryo using RNAi and electroporation. Nat. Biotechnol., 21: 93-6.
124. Pelegrin, M., Gros, L., Dreja, H. and Piechaczyk, M., (2004). Monoclonal antibody-based genetic immunotherapy. Curr. Gene Ther., 4: 347-56.
125. Peng, B., Zhao, Y., Lu, H., Pang, W. and Xu, Y., (2005). In vivo plasmid DNA electroporation resulted in transfection of satellite cells and lasting transgene expression in regenerated muscle fibers. Biochem. Biophys. Res. Commun., 338: 1490-8.
126. Perez, N., Bigey, P., Scherman, D., Danos, O., Piechaczyk, M. and Pelegrin, M., (2004). Regulatable systemic production of monoclonal antibodies by in vivo muscle electroporation. Genet. Vaccines Ther., 2: 2.
127. Pradat, P. F., Kennel, P., Naimi-Sadaoui, S., Finiels, F., Scherman, D., Orsini, C., Delaere, P., Mallet, J. and Revah, F., (2002). Viral and non-viral gene therapy partially prevents experimental cisplatin-induced neuropathy. Gene Ther., 9: 1333-7.
128. Prud'homme, G. J., Glinka, Y., Khan, A. S. and Draghia-Akli, R., (2006). Electroporation-enhanced nonviral gene transfer for the prevention or treatment of immunological, endocrine and neoplastic diseases. Curr. Gene Ther., 6: 243-73.
129. Rao. M.. Baraban. J. H.. Raiaii. F. and Sockanathan. S.. (2004). In vivo comparative study of RNAi methodologies by in ovo electroporation in the chick embryo. Dev. Dyn., 231: 592-600.
130. Rebersek, M., Cofer, T., Cemazar, M., Kranjc, S. and Sersa, G., (2004). Electrochemotherapy with cisplatin of cutaneous tumor lesions in breast cancer. AntiCancer Drugs., 15: 593-7.
131. Rizzuto, G., Cappelletti, M., Maione, D., Savino, R., Lazzaro, D., Costa, P., Mathiesen, I., Cortese, R., Ciliberto, G., Laufer, R., La Monica, N. and Fattori, E., (1999). Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. Proc. Natl. Acad. Sci. USA, 96: 6417-22.
132. Rizzuto, G., Cappelletti, M., Mennuni, C., Wiznerowicz, M., DeMartis, A., Maione, D., Ciliberto, G., La Monica, N. and Fattori, E., (2000). Gene electrotransfer results in a high-level transduction of rat skeletal muscle and corrects anemia of renal failure. Hum. Gene Ther., 11: 1891-900.
133. Rols, M. P., Delteil, C., Golzio, M. and Teissie, J., (1998). Control by ATP and ADP of voltage-induced mammalian-cell-membrane permeabilization, gene transfer and resulting expression. Eur. J. Biochem., 254: 382-8.
134. Rols, M. P., Tamzali, Y. and Teissie, J., (2002). Electrochemotherapy of horses. A preliminary clinical report. Bioelectrochemistry., 55: 101-5.
135. Roorda, B. D., Hesselink, M. K., Schaart, G., Moonen-Kornips, E., Martinez-Martinez, P., Losen, M., De Baets, M. H., Mensink, R. P. and Schrauwen, P., (2005). DGAT1 overexpression in muscle by in vivo DNA electroporation increases intramyocellular lipid content. J. Lipid Res., 46: 230-6.
136. Rubenstrunk, A., Mahfoudi, A. and Scherman, D., (2004). Delivery of electric pulses for DNA electrotransfer to mouse muscle does not induce the expression of stress related genes. Cell Biol. Toxicol., 20: 25-31.
137. Rubenstrunk, A., Trollet, C., Orsini, C. and Scherman, D., (2005). Positive in vivo heterologous gene regulation by electric pulses delivery with metallothionein I gene promoter. J. Gene Med., 7: 1565-72.
138. Sadasivam, G., Willmann, R., Lin, S., Erb-Vogtli, S., Kong, X. C., Ruegg, M. A. and Fuhrer, C., (2005). Src-family kinases stabilize the neuromuscular synapse in vivo via protein interactions, phosphorylation, and cytoskeletal linkage of acetylcholine receptors. J. Neurosci., 25: 10479-93.
139. Saidenberg-Kermanac'h, N., Bessis, N., Deleuze, V., Bloquel, C., Bureau, M., Scherman, D. and Boissier, M. C., (2003). Efficacy of interleukin 10 gene electrotransfer into skeletal muscle in mice with collagen induced arthritis. J. Gene Med., 5: 164-71.
140. Satkauskas, S., Bureau, M. F., Mahfoudi, A. and Mir, L. M., (2001). Slow accumulation of plasmid in muscle cells: supporting evidence for a mechanism of DNA uptake by receptor-mediated endocytosis. Mol. Ther., 4: 317-23.
141. Satkauskas, S., Bureau, M. F., Puc, M., Mahfoudi, A., Scherman, D., Miklavcic, D. and Mir, L. M., (2002). Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis. Mol. Ther., 5: 133-40.
142. Schakman, O., Gilson, H., de Coninck, V., Lause, P., Verniers, J., Havaux, X., Ketelslegers, J. M. and Thissen, J. P., (2005). Insulin-like growth factor-I gene transfer by electroporation prevents skeletal muscle atrophy in glucocorticoid-treated rats. Endocrinology, 146: 1789-97.
143. Scheerlinck, J. P., Karlis, J., Tjelle, T. E., Presidente, P. J., Mathiesen, I. and Newton, S. E., (2004). In vivo electroporation improves immune responses to DNA vaccination in sheep. Vaccine, 22: 1820-5.
144. Sersa, G., Stabuc, B., Cemazar, M., Miklavcic, D. and Rudolf, Z., (2000). Electrochemotherapy with cisplatin: the systemic antitumour effectiveness of cisplatin can be potentiated locally by the application of electric pulses in the treatment of malignant melanoma skin metastases. Melanoma Res., 10: 381-5.
145. Silvestre, J. S., Mallat, Z., Duriez, M., Tamarat, R., Bureau, M. F., Scherman, D., Duverger, N., Branellec, D., Tedgui, A. and Levy, B. I., (2000). Antiangiogenic effect of interleukin-10 in ischemia-induced angiogenesis in mice hindlimb. Circ Res., 87: 448-52.
146. Silvestre, J. S., Tamarat, R., Ebrahimian, T. G., Le-Roux, A., Clergue, M., Emmanuel, F., Duriez, M., Schwartz, B., Branellec, D. and Levy, B. I., (2003). Vascular endothelial growth factor-B promotes in vivo angiogenesis. Circ. Res., 93: 114-23.
147. Stieger, K., Le Meur, G., Lasne, F., Weber, M., Deschamps, J. Y., Nivard, D., Mendes-Madeira, A., Provost, N., Martin, L., Moullier, P. and Rolling, F., (2006). Long-term doxycycline-regulated transgene expression in the retina of nonhuman primates following subretinal injection of recombinant AAV vectors. Mol. Ther., 13: 967-75.
148. Sugano, M., Hata, T., Tsuchida, K., Suematsu, N., Oyama, J., Satoh, S. and Makino, N., (2004). Local delivery of soluble TNF-alpha receptor 1 gene reduces infarct size following ischemia/reperfusion injury in rats. Mol. Cell Biochem., 266: 127-32.
149. Sukharev, S. I., Klenchin, V. A., Serov, S. M., Chernomordik, L. V. and Chizmadzhev Yu, A., (1992). Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores. Biophys. J., 63: 1320-7.
150. Sun, W., Wang, L., Zhang, Z., Chen, M. and Wang, X., (2003). Intramuscular transfer of naked calcitonin gene-related peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice. Eur. J. Immunol., 33: 233-42.
151. Tang, D. C., DeVit, M. and Johnston, S. A., (1992). Genetic immunization is a simple method for eliciting an immune response. Nature, 356: 152-4.
152. Tavakoli, R., Gazdhar, A., Pierog, J., Bogdanova, A., Gugger, M., Pringle, I. A., Gill, D. R., Hyde, S. C., Genoni, M. and Schmid, R. A., (2006). Electroporation-mediated interleukin-10 overexpression in skeletal muscle reduces acute rejection in rat cardiac allografts. J. Gene Med., 8: 242-248.
153. Tawk, M., Tuil, D., Torrente, Y., Vriz, S. and Paulin, D., (2002). High-efficiency gene transfer into adult fish: a new tool to study fin regeneration. Genesis., 32: 27-31.
154. Teissie, J., Eynard, N., Gabriel, B. and Rols, M. P., (1999). Electropermeabilization of cell membranes. Adv. Drug Deliv. Rev., 35: 3-19.
155. Teissie, J. and Rols, M. P., (1993). An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization. Biophys. J., 65: 409-13.
156. Tollefsen, S., Tjelle, T., Schneider, J., Harboe, M., Wiker, H., Hewinson, G., Huygen, K. and Mathiesen, I., (2002). Improved cellular and humoral immune responses against Mycobacterium tuberculosis antigens after intramuscular DNA immunisation combined with muscle electroporation. Vaccine, 20: 3370-8.
157. Tollefsen, S., Vordermeier, M., Olsen, I., Storset, A. K., Reitan, L. J., Clifford, D., Lowrie, D. B., Wiker, H. G., Huygen, K., Hewinson, G., Mathiesen, I. and Tjelle, T. E., (2003). DNA injection in combination with electroporation: a novel method for vaccination of farmed ruminants. Scand. J. Immunol., 57: 229-38.
158. Trollet, C., Bigey, P. and Scherman, D., (2005). Electrotransfection: an overview. DNA-Pharmaceuticals Wiley., 1: 189-218.
159. Trollet, C., Ibanez-Ruiz, M., Bloquel, C., Valin, G., Scherman, D. and Bigey, P., (2004). Regulation of gene expression using a conditional RNA antisense strategy. J. Genome Sci. Tech.., 3: 1-13.
160. Ulmer, J. B., Donnelly, J. J., Parker, S. E., Rhodes, G. H., Felgner, P. L., Dwarki, V. J., Gromkowski, S. H., Deck, R. R., DeWitt, C. M. and Friedman, A., (1993). Heterologous protection against influenza by injection of DNA encoding a viral protein. Science, 259: 1745-9.
161. Umeda, Y., Marui, T., Matsuno, Y., Shirahashi, K., Iwata, H., Takagi, H., Matsumoto, K., Nakamura, T., Kosugi, A., Mori, Y. and Takemura, H., (2004). Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice. Lab. Invest, 84: 836-44.
162. van Drunen Littel-van den Hurk, S., Babiuk, S. L. and Babiuk, L. A., (2004). Strategies for improved formulation and delivery of DNA vaccines to veterinary target species. Immunol. Rev., 199: 113-25.
163. Vicat, J. M., Boisseau, S., Jourdes, P., Laine, M., Wion, D., Bouali-Benazzouz, R., Benabid, A. L. and Berger, F., (2000). Muscle transfection by electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression. Hum. Gene Ther., 11: 909-16.
164. Vilquin, J. T., Kennel, P. F., Paturneau-Jouas, M., Chapdelaine, P., Boissel, N., Delaere, P., Tremblay, J. P., Scherman, D., Fiszman, M. Y. and Schwartz, K., (2001). Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies. Gene Ther., 8: 1097-107.
165. Wang, Z., Troilo, P. J., Wang, X., Griffiths, T. G., Pacchione, S. J., Barnum, A. B., Harper, L. B., Pauley, C. J., Niu, Z., Denisova, L., Follmer, T. T., Rizzuto, G., Ciliberto, G., Fattori, E., Monica, N. L., Manam, S. and Ledwith, R. J., (2004). Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation. Gene Ther., 11: 711-21.
166. Watanabe, K., Nakazawa, M., Fuse, K., Hanawa, H., Kodama, M., Aizawa, Y., Ohnuki, T., Gejyo, F., Maruyama, H. and Miyazaki, J., (2001). Protection against autoimmune myocarditis by gene transfer of interleukin- 10 by electroporation. Circulation, 104: 1098-100.
167. Weber, W. and Fussenegger, M., (2002). Artificial mammalian gene regulation networks-novel approaches for gene therapy and bioengineering. J. Biotechnol., 98: 161-87.
168. Widera, G., Austin, M., Rabussay, D., Goldbeck C., Barnett; S. W., Chen, M., Leung, L., Otten, G. R., Thudium, K., Selby, M. J. and Ulmer, J. B., (2000). Increased DNA vaccine delivery and immunogenicity by electroporation in vivo. J. Immunol., 164: 4635-40.
169. Wong, S. H., Lowes, K. N., Quigley, A. F., Marotta, R., Kita, M., Byrne, E., Kornberg, A. J., Cook, M. J. and Kapsa, R. M., (2005). DNA electroporation in vivo targets mature fibres in dystrophic mdx muscle. Neuromuscul. Disord., 15: 630-41.
170. Xiang, L., Murai, A. and Muramatsu, T., (2005). Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice. Int. J. Mol. Med., 16: 1015-20.
171. Xiang, L., Murai, A., Sugahara, K., Yasui, A. and Muramatsu, T., (2003). Effects of leptin gene expression in mice in vivo by electroporation. and hydrodynamics-based gene delivery. Biochem. Biophys. Res. Commun., 307: 440-5.
172. Xue, F., Takahara, T., Yata, Y., Kuwabara, Y., Shinno, E., Nonome, K., Minemura, M., Takahara, S., Li, X., Yamato, E. and Watanabe, A., (2003). Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy. Gut, 52: 694-700.
173. Xue, F., Takahara, T., Yata, Y., Minemura, M., Morioka, C. Y., Takahara, S., Yamato, E., Dono, K. and Watanabe, A., (2002). Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation. Gut, 50: 558-62.
174. Yamamoto, M., Kobayashi, Y., Li, M., Niwa, H., Mitsuma, N., Ito, Y., Muramatsu, T. and Sobue, G., (2001). In vivo gene electroporation of glial cell line-derived neurotrophic factor (GDNF) into skeletal muscle of SOD1 mutant mice. Neurochem. Res., 26: 1201-7.
175. Zhang, G. H., Tan, X. F., Shen, D., Zhao, S. Y., Shi, Y. L., Jin, C. K., Sun, W. G., Guo, Y. H., Chen, K. H. and Tang, J., (2003). Gene expression and antitumor effect following im electroporation delivery of human interferon alpha 2 gene. Acta. Pharmacol. Sin., 24: 891-6.