Directed Evolution of an Anti-prion Protein scFv Fragment to an Affinity of 1 pM and its Structural Interpretation

Journal of Molecular Biology

Volumn 363, Issue 1, 2006, Pages 75-97

  Luginbühl, Béatrice ^a   Kanyo, Zoltan ^b   Jones, R Mark ^c   Fletterick, Robert J ^b   Prusiner, Stanley B ^b,d   Cohen, Fred E ^b,d   Williamson, R Anthony ^e   Burton, Dennis R ^e   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Sapidyne Instruments Incorporated   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

antibody engineering; Biacore; crystal structure; KinExA; prion

Indexed keywords

PRION PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY LIBRARY; ARTICLE; BINDING AFFINITY; BIOSENSOR; CRYSTAL STRUCTURE; DENATURATION; DNA SHUFFLING; MOLECULAR EVOLUTION; MOLECULAR MODEL; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STRUCTURE; RIBOSOME;

BOS TAURUS;

EID: 33748793940     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2006.07.027     Document Type: Article

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