The transcriptional repressor Nkx6.1 also functions as a deoxyribonucleic acid context-dependent transcriptional activator during pancreatic β-cell differentiation: Evidence for feedback activation of the nkx6.1 gene by Nkx6.1

Molecular Endocrinology

Volumn 18, Issue 6, 2004, Pages 1363-1375

  Iype, Tessy ^a   Taylor, David G ^b   Ziesmann, Suzanne M ^a   Garmey, James C ^a   Watada, Hirotaka ^c   Mirmira, Raghavendra G ^a,b,d  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c JUNTENDO UNIVERSITY   (Japan)

^d UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID; DNA; HOMEODOMAIN PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NKX6.1; TRANSCRIPTION FACTOR PDX 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AT RICH SEQUENCE; CARBOXY TERMINAL SEQUENCE; CELL SPECIFICITY; CHROMATIN; CONTROLLED STUDY; DNA PROTEIN COMPLEX; ENHANCER REGION; FEEDBACK SYSTEM; GC RICH SEQUENCE; GEL MOBILITY SHIFT ASSAY; GENE ACTIVATION; GENE CONTROL; GENE EXPRESSION REGULATION; GENE IDENTIFICATION; GENE REPRESSION; GENE SEQUENCE; IMMUNOPRECIPITATION; IN VITRO STUDY; IN VIVO STUDY; MAMMAL CELL; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN FUNCTION; REPORTER GENE; TRANSCRIPTION INITIATION; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION REGULATION; TRANSCRIPTION TERMINATION; TRANSLATION REGULATION;

ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; DNA; ENHANCER ELEMENTS (GENETICS); ESCHERICHIA COLI; GENES, REPORTER; GENETIC VECTORS; HELA CELLS; HOMEODOMAIN PROTEINS; HUMANS; IMMUNOPRECIPITATION; ISLETS OF LANGERHANS; LUCIFERASES; MICE; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NIH 3T3 CELLS; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC; TRANSFECTION;

ANIMALIA; MAMMALIA;

EID: 2542484345     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/me.2004-0006     Document Type: Article

References (45)

1. Chakrabarti SK, Mirmira RG 2003 Transcription factors direct the development and function of pancreatic β cells. Trends Endocrinol Metab 14:78-84
2. Sander M, Sussel L, Conners J, Scheel D, Kalamaras J, Dela Cruz F, Schwitzgebel V, Hayes-Jordan A, German M 2000 Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of β-cell formation in the pancreas. Development 127:5533-5540
3. Kim SK, Selleri L, Lee JS, Zhang AY, Gu X, Jacobs Y, Cleary ML 2002 Pbx1 inactivation disrupts pancreas development and in Ipf1-deficient mice promotes diabetes mellitus. Nat Genet 30:430-435
4. Ahlgren U, Pfaff SL, Jessell TM, Edlund T, Edlund H 1997 Independent requirement for ISL1 in formation of pancreatic mesenchyme and islet cells. Nature 385:257-260
5. Sussel L, Kalamaras J, Hartigan-O'Connor DJ, Meneses JJ, Pedersen RA, Rubenstein JL, German MS 1998 Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic β cells. Development 125:2213-2221
6. Harrison KA, Thaler J, Pfaff SL, Gu H, Kehrl JH 1999 Pancreas dorsal lobe agenesis and abnormal islets of Langerhans in Hlxb9-deflcient mice. Nat Genet 23:71-75
7. Li H, Arber S, Jessell TM, Edlund H 1999 Selective agenesis of the dorsal pancreas in mice lacking homeobox gene Hlxb9. Nat Genet 23:67-70
8. Jonsson J, Carlsson L, Edlund T, Edlund H 1994 Insulin-promoter-factor 1 is required for pancreas development in mice. Nature 371:606-609
9. Offield MF, Jetton TL, Labosky PA, Ray M, Stein RW, Magnuson MA, Hogan BL, Wright CV 1996 PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development 122:983-995
10. Sosa-Pineda B, Chowdhury K, Torres M, Oliver G, Gruss P 1997 The Pax4 gene is essential for differentiation of insulin-producing β cells in the mammalian pancreas. Nature 386:399-402
11. Sander M, Paydar S, Ericson J, Briscoe J, Berber E, German M, Jessell TM, Rubenstein JL 2000 Ventral neural patterning by Nkx homeobox genes: Nkx6.1 controls somatic motor neuron and ventral interneuron fates. Genes Dev 14:2134-2139
12. Vallstedt A, Muhr J, Pattyn A, Pierani A, Mendelsohn M, Sander M, Jessell TM, Ericson J 2001 Different levels of repressor activity assign redundant and specific roles to Nkx6 genes in motor neuron and interneuron specification. Neuron 31:743-755
13. Jorgensen MC, Vestergard Petersen H, Ericson J, Madsen OD, Serup P 1999 Cloning and DNA-binding properties of the rat pancreatic β-cell-specific factor Nkx6.1. FEBS Lett 461:287-294
14. Mirmira RG, Watada H, German MS 2000 β-Cell differentiation factor Nkx6.1 contains distinct DNA binding interference and transcriptional repression domains. J Biol Chem 275:14743-14751
15. Muhr J, Andersson E, Persson M, Jessell TM, Ericson J 2001 Groucho-mediated transcriptional repression establishes progenitor cell pattern and neuronal fate in the ventral neural tube. Cell 104:861-873
16. Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J 2003 Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development 130:4149-4159
17. Oster A, Jensen J, Edlund H, Larsson LI 1998 Homeobox gene product Nkx 6.1 immunoreactivity in nuclei of endocrine cells of rat and mouse stomach. J Histochem Cytochem 46:717-721
18. Watada H, Mirmira RG, Leung J, German MS 2000 Transcriptional and translational regulation of β-cell differentiation factor Nkx6.1. J Biol Chem 275:34224-34230
19. Gerrish KE, Cissell MA, Stein R 2001 The role of hepatic nuclear factor 1a and PDX-1 in transcriptional regulation of the pdx-1 gene. J Biol Chem 276:47775-47784
20. Smith SB, Watada H, Scheel DW, Mrejen C, German MS 2000 Autoregulation and maturity onset diabetes of the young transcription factors control the human PAX4 promoter. J Biol Chem 275:36910-36919
21. Aota S, Nakajima N, Sakamoto R, Watanabe S, Ibaraki N, Okazaki K 2003 Pax6 autoregulation mediated by direct interaction of Pax6 protein with the head surface ectoderm-specific enhancer of the mouse Pax6 gene. Dev Biol 257:1-13
22. Smith SB, Watada H, German MS 2004 Neurogenin3 activates the islet differentiation program while repressing its own expression. Mol Endocrinol 18:142-149
23. Stringer KF, Ingles CJ, Greenblatt J 1990 Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID. Nature 345:783-786
24. Roberts SG, Ha I, Maldonado E, Reinberg D, Green MR 1993 Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation. Nature 363:741-744
25. Ohneda K, Ee H, German M 2000 Regulation of insulin gene transcription. Semin Cell Dev Biol 11:227-233
26. Treisman J, Harris E, Wilson D, Desplan C 1992 The homeodomain: a new face for the helix-turn-helix? Bioessays 14:145-150
27. Lin Y, Ince TA, Scotto KW 2001 Optimization of a versatile in vitro transcription assay for the expression of multiple start site TATA-less promoters. Biochemistry 40:12959-12966
28. Uhler J, Garbern J, Yang L, Kamholz J, Mellerick DM 2002 Nk6, a novel Drosophila homeobox gene regulated by vnd. Mech Dev 116:105-116
29. Wang H, Maechler P, Ritz-Laser B, Hagenfeldt KA, Ishihara H, Philippe J, Wollheim CB 2001 Pdx1 level defines pancreatic gene expression pattern and cell lineage differentiation. J Biol Chem 276:25279-25286
30. Ahlgren U, Jonsson J, Jonsson L, Simu K, Edlund H 1998 β-Cell-specific inactivation of the mouse Ipf1/Pdx1 gene results in loss of the β-cell phenotype and maturity onset diabetes. Genes Dev 12:1763-1768
31. Komuro I, Schalling M, Jahn L, Bodmer R, Jenkins NA, Copeland NG, Izumo S 1993 Gtx: a novel murine homeobox-containing gene, expressed specifically in glial cells of the brain and germ cells of testis, has a transcriptional repressor activity in vitro for a serum-inducible promoter. EMBO J 12:1387-1401
32. Greenall A, Willingham N, Cheung E, Boam DS, Sharrocks AD 2001 DNA binding by the ETS-domain transcription factor PEA3 is regulated by intramolecular and intermolecular protein.protein interactions. J Biol Chem 276:16207-16215
33. Stemmer C, Schwander A, Bauw G, Fojan P, Grasser KD 2002 Protein kinase CK2 differentially phosphorylates maize chromosomal high mobility group B (HMGB) proteins modulating their stability and DNA interactions. J Biol Chem 277:1092-1098
34. Das D, Scovell WM 2001 The binding interaction of HMG-1 with the TATA-binding protein/TATA complex. J Biol Chem 276:32597-32605
35. Goetz TL, Gu TL, Speck NA, Graves BJ 2000 Autoinhibition of Ets-1 is counteracted by DNA binding cooperativity with core-binding factor α2, Mol Cell Biol 20:81-90
36. Kaczynski J, Cook T, Urrutia R 2003 Sp1- and Kruppel-like transcription factors. Genome Biol 4:206
37. Suzuki M, Ueno N, Kuroiwa A 2003 Hox proteins functionally cooperate with the GC box-binding protein system through distinct domains. J Biol Chem 278:30148-30156
38. Majello B, De Luca P, Lania L 1997 Sp3 is a bifunctional transcription regulator with modular independent activation and repression domains. J Biol Chem 272:4021-4026
39. Petersen JM, Skalicky JJ, Donaldson LW, McIntosh LP, Alber T, Graves BJ 1995 Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an α helix. Science 269:1866-1869
40. Sakai DD, Helms S, Carlstedt-Duke J, Gustafsson JA, Rottman FM, Yamamoto KR 1988 Hormone-mediated repression: a negative glucocorticoid response element from the bovine prolactin gene. Genes Dev 2:1144-1154
41. German MS, Wang J, Chadwick RB, Rutter WJ 1992 Synergistic activation of the insulin gene by a LIM-homeo domain protein and a basic helix-loop-helix protein: building a functional insulin minienhancer complex. Genes Dev 6:2165-2176
42. Chakrabarti SK, James JC, Mirmira RG 2002 Quantitative assessment of gene targeting in vitro and in vivo by the pancreatic transcription factor, Pdx1. Importance of chromatin structure in directing promoter binding. J Biol Chem 277:13286-13293
43. Morrison TB, Weis JJ, Wittwer CT 1998 Quantification of low-copy transcripts by continuous SYBR green I monitoring during amplification. Biotechniques 24:954-958, 960, 962
44. German MS, Moss LG, Wang J, Rutter WJ 1992 The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical β-cell nuclear complexes. Mol Cell Biol 12:1777-1788
45. Chakrabarti SK, Francis J, Ziesmann SM, Garmey JC, Mirmira RG 2003 Covalent histone modifications underlie the developmental regulation of insulin gene transcription in pancreatic β cells. J Biol Chem 278:23617-23623