Reduced oxygen supply increases process stability and product yield with recombinant Pichia pastoris

Biotechnology Progress

Volumn 20, Issue 6, 2004, Pages 1766-1775

  Trentmann, Oliver ^b,c   Khatri, Narendar K ^a   Hoffmann, Frank ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b Novoplant GmbH   (Germany)

^c Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELLS; DEGRADATION; ESCHERICHIA COLI; METABOLISM; METHANOL; OXYGEN; PH EFFECTS; TITRATION;

CULTURE INTEGRITY; FEEDING RATE; FLOW CYTOMETRY; HIGH-CELL-DENSITY CULTIVATION;

ANTIBODIES;

IMMUNOGLOBULIN FRAGMENT; OXYGEN;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; CELL PROLIFERATION; COMPARATIVE STUDY; CULTURE TECHNIQUE; ESCHERICHIA COLI; EVALUATION; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; OXYGEN CONSUMPTION; PHYSIOLOGY; PICHIA; PROTEIN ENGINEERING;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, FUNGAL; IMMUNOGLOBULIN FRAGMENTS; OXYGEN; OXYGEN CONSUMPTION; PICHIA; PROTEIN ENGINEERING;

ESCHERICHIA COLI; PICHIA; PICHIA PASTORIS;

EID: 10044227272     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp049711h     Document Type: Article

References (37)

1. Sun, R.; Anderson, T. J.; Erickson, A. K.; Nelson, E. A.; Francis, D. H. Inhibition of adhesion of Escherichia coli k88ac fimbria to its receptor, intestinal mucin-type glycoproteins, by a monoclonal antibody directed against a variable domain of the fimbria. Infect. Immun. 2000, 68, 3509-3515.
2. Lin Cereghino, G. P.; Lin Cereghino, J.; Ilgen, C.; Cregg, J. M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 2002, 13, 329-332.
3. Lin Cereghino, G. P., Sunga, A. J., Lin Cereghino, J., Cregg, J. M. Expression of foreign genes in the yeast Pichia pastoris. In Genetic Engineering: Principles and Methods; Setlow, J. K., Ed.; Kluwer Academic/Plenum Publishers: London, 2001; Vol. 23, pp 157-169.
4. Hasslacher, M.; Schall, M.; Hayn, M.; Bona, R.; Rumbold, K.; Lückl, J.; Griengl, H.; Kohlwein, S. D.; Schwab, H. High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts. Protein Expression Purif. 1997, 11, 61-71.
5. Werten, M. W. T.; van den Bosch, T. J.; Wind, R. D.; Mooibroek, H.; de Wolf, F. A. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 1999, 15, 1087-1096.
6. Fischer, R.; Drossard, J.; Emans, N.; Commandeur, U.; Hellwig, S. Towards molecular farming in the future: Pichia pastoris-based production of single-chain antibody fragments. Biotechnol. Appl. Biochem. 1999, 30, 117-120.
7. Freyre, F. M.; Vázquez, J. E.; Ayala, M.; Canaán-Haden, L.; Bell, H.; Rodríguez, I.; González, A.; Cintado, A.; Gavilondo, J. V. Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J. Biotechnol. 2000, 76, 157-163.
8. Bushell, M. E.; Rowe, M.; Avignone-Rossa, C. A.; Wardell, J. N. Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris. Biotechnol. Bioeng. 2003, 82, 678-683.
9. Lin Cereghino, J.; Cregg, J. M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 2000, 24, 45-66.
10. Thiry, M.; Cingolani, D. Optimizing scale-up fermentation processes. Tibtech 2002, 20, 103-105.
11. Curvers, S.; Brixius, P.; Klauser, T.; Thömmes, J.; Weuster-Botz, D.; Takors, R.; Wandrey, C. Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation. Biotechnol. Prog. 2001, 17, 495-502.
12. Lee, C. Y.; Lee, S. J.; Jung, K. H.; Katoh, S.; Lee, E. K. High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris. Proc. Biochem. 2003, 38, 1147-1154.
13. Jahic, M.; Rotticci-Mulder, J. C.; Martinelle, M.; Hult, K.; Enfors, S.-O. Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein. Bioprocess Biosyst. Eng. 2002, 24, 385-393.
14. Trinh, L. B.; Phue, J. N.; Shiloach, J. Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol. Bioeng. 2003, 82, 438-444.
15. Guarna, M. M.; Lesnicki, G. J.; Tam, B. M.; Robinson, J.; Radziminski, C. Z.; Hasenwinkle, D.; Boraston, A.; Jervis, E.; MacGillivray, R. T. A.; Turner, R. F. B.; Kilburn, D. G. Online monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris. Biotechnol. Bioeng. 1997, 56, 279-286.
16. 2-glycoprotein I domain V by a recombinant Pichia pastoris: A simple system for the control of methanol concentration using a semiconductor gas sensor. J. Ferment. Bioeng. 1998, 86, 482-487.
17. Zhou, X.-S.; Zhang, Y.-X. Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnol. Lett. 2002, 24, 1449-1453.
18. Stratton, J., Chiruvolu, V., Meagher, M. High cell-density fermentation. In Pichia Protocols; Higgins, D. R., Cregg, J. M., Eds.; Humana Press: Totowa, NJ, 1998; pp 107-120.
19. Jahic, M.; Wallberg, F.; Bollok, M.; Garcia, P.; Enfors, S.-O. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Factories 2003, 2, 6
20. Soriano, E.; Borth, N.; Katinger, H.; Mattanovich, D. Optimization of recombinant protein expression level in Escherichia coli by flow cytometry and cell sorting. Biotechnol. Bioeng. 2002, 80, 93-99.
21. Hewitt, C. J.; Nebe-von Caron, G.; Axelsson, B.; McFarlane, C. M.; Nienow, A. W. Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multi-parameter flow cytometry: Effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration. Biotechnol. Bioeng. 2000, 70, 381-390.
22. Onyeaka, H.; Nienow, A. W.; Hewitt, C. J. Further studies related to the scale-up of high cell density Escherichia coli fed-batch fermentations: The additional effect of a changing microenvironment when using aqueous ammonia to control pH. Biotechnol. Bioeng. 2003, 84, 474-484.
23. Soriano, E.; Borth, N.; Katinger, H.; Mattanovich, D. Flow cytometric analysis of metabolic stress effects due to recombinant plasmids and proteins in Escherichia coli production strains. Metab. Eng. 1999, 1, 270-274.
24. Borth, N.; Mitterbauer, R.; Mattanovich, D.; Kramer, W.; Bayer, K.; Katinger, H. Flow cytometric analysis of bacterial physiology during induction of foreign protein synthesis in recombinant Escherichia coli cells. Cytometry 1998, 31, 125-129.
25. Hewitt, C. J.; Nebe-von Caron, G.; Nienow, A. W.; McFarland, C. M. Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures. Biotechnol. Bioeng. 1999, 63, 705-711.
26. Alberghina, L.; Ranzi, B. M.; Porro, D.; Martegani, E. Flow cytometry and cell cycle kinetics in continuous and fed-batch fermentations of budding yeast. Biotechnol. Prog. 1991, 7, 299-304.
27. Ranzi, B. M.; Compagno, C.; Martegani, E. Analysis of protein and cell volume distribution in glucose-limited continuous cultures of budding yeast. Biotechnol. Bioeng. 1986, 28, 185-190.
28. Müller, S.; Hutter, K. J.; Bley, T.; Petzold, L.; Babel, W. Dynamics of yeast cell states during proliferation and non proliferation periods in a brewing reactor monitored by multidimensional flow cytometry. Bioprocess Biosyst. Eng. 1997, 17, 287-293.
29. Wittrup, K. D.; Bailey, J. E.; Ratzkin, B.; Patel, A. Propagation of an amplifiable recombinant plasmid in Saccharomyces cerevisiae: flow cytometry studies and segregated modeling. Biotechnol. Bioeng. 1990, 35, 565-577.
30. Chau, T. L.; Guillán, A.; Roca, E.; Núñez, M. J.; Lema, J. M. Population dynamics of a continuous fermentation of recombinant Saccharomyces cerevisiae using flow cytometry. Biotechnol. Prog. 2001, 17, 951-957.
31. Hohenblum, H.; Borth, N.; Mattanovich, D. Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J. Biotechnol. 2003, 102, 281-290.
32. Zhang, W.; Bevins, M. A.; Plantz, B. A.; Smith, L. A.; Meagher, M. M. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of Botulinum neurotoxin, Serotype A. Biotechnol. Bioeng. 2000, 70, 1-8.
33. Hellwig, S.; Emde, F.; Raven, N. P. G.; Henke, M.; van der Logt, P.; Fischer, R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 2001, 74, 344-352.
34. Gonzalez, E. A.; Vazquez, F.; Ignacio Garabal, J.; Blanco, J. Isolation of K88 antigen variants (ab, ac, ad) from porcine enterotoxigenic Escherichia coli belonging to different serotypes. Microbiol. Immunol. 1995, 39, 937-942.
35. Hong, F.; Meinander, N. Q.; Jönsson, L. J. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol. Bioeng. 2002, 79, 438-449.
36. Cunha, A. E.; Clemente, J. J.; Gomes, R.; Pinto, F.; Thomaz, M.; Miranda, S.; Pinto, R.; Moosmayer, D.; Donner, P.; Carrondo, M. J. T. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol. Bioeng. 2004, 86, 458-467.
37. Chiruvolu, V.; Cregg, J. M.; Meagher, M. M. Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations. Enzyme Microb. Technol. 1997, 21, 277-283.