Further Studies Related to the Scale-up of High Cell Density Escherichia coli Fed-Batch Fermentations: The Additional Effect of a Changing Microenvironment When Using Aqueous Ammonia to Control pH

Biotechnology and Bioengineering

Volumn 84, Issue 4, 2003, Pages 474-484

  Onyeaka, Helen ^a   Nienow, Alvin W ^a   Hewitt, Christopher J ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Escherichia coli; Fed batch; Flow cytometry; High cell density; Membrane integrity; Membrane potential; Microenvironment; pH; Scale up; Viability

Indexed keywords

AMMONIA; FERMENTATION; GLUCOSE; OXYGEN; PH EFFECTS; PHYSIOLOGY;

CELL DENSITY;

ESCHERICHIA COLI;

AMMONIA; DISSOLVED OXYGEN; GLUCOSE;

ARTICLE; BIOMASS; BIOREACTOR; CELL DENSITY; CELL FUNCTION; CELL VIABILITY; COMPARTMENT MODEL; ESCHERICHIA COLI; FED BATCH FERMENTATION; FLOW CYTOMETRY; HIGH CELL DENSITY; INCUBATION TIME; MEMBRANE POTENTIAL; MICROBIOLOGICAL EXAMINATION; MICROENVIRONMENT; NONHUMAN; PH; PLUG FLOW REACTOR; SIMULATION; STIRRED REACTOR;

AMMONIA; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIVISION; COMPUTER SIMULATION; ENVIRONMENT; ESCHERICHIA COLI; FLOW CYTOMETRY; GLUCOSE; HYDROGEN-ION CONCENTRATION; MODELS, BIOLOGICAL; OXYGEN CONSUMPTION; PILOT PROJECTS; WATER;

ESCHERICHIA COLI;

EID: 0142073266     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10805     Document Type: Article

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