Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry: Effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration

Biotechnology and Bioengineering

Volumn 70, Issue 4, 2000, Pages 381-390

  Hewitt, Christopher J ^a   Caron, Gerhard Nebe Von ^b   Axelsson, Britta ^c   McFarlane, Caroline M ^a   Nienow, Alvin W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNILEVER RESEARCH   (United Kingdom)

^c ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Escherichia coli; Fed batch; Flow cytometry; High cell density; Membrane integrity; Membrane potential; Microenvironment; Scale up; Viability

Indexed keywords

BIOMASS; CELL MEMBRANES; ESCHERICHIA COLI; GLUCOSE; OXYGEN; PHYSIOLOGY; SUBSTRATES;

FED BATCH FERMENTATION; FLOW CYTOMETRY; HIGH-CELL-DENSITY; MEMBRANE POTENTIAL;

FERMENTATION;

DISSOLVED OXYGEN; GLUCOSE;

ARTICLE; BIOMASS; BIOREACTOR; CELL DENSITY; CELL FUNCTION; CELL MEMBRANE PERMEABILITY; CELL VIABILITY; COMPARTMENT MODEL; CONTROLLED STUDY; ENVIRONMENT; ESCHERICHIA COLI; FERMENTATION; FLOW CYTOMETRY; NONHUMAN; OXYGEN CONCENTRATION; STRESS;

BIOTECHNOLOGY; CELL DIVISION; ESCHERICHIA COLI; FERMENTATION; FLOW CYTOMETRY; GLUCOSE; OXYGEN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034694211     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001120)70:4<381::AID-BIT3>3.0.CO;2-0     Document Type: Article

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