Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1

Molecular and Cellular Biology

Volumn 23, Issue 20, 2003, Pages 7363-7376

  Li, Chin ^a   Lin, Ru Inn ^a   Lai, Ming Chih ^a   Ouyang, Pin ^b   Tarn, Woan Yuh ^a  

^a INSTITUTE OF BIOMEDICAL SCIENCES   (Taiwan)

^b CHANG GUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; NUCLEAR PROTEIN; OLIGONUCLEOTIDE; PNN PROTEIN; POLYADENYLATED RNA; RIBONUCLEASE H; RIBONUCLEOPROTEIN; RIBONUCLEOPROTEIN S1; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; DESMOSOME; EXON; HETEROKARYON; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; REPORTER GENE; RNA METABOLISM; RNA PROCESSING; RNA SPLICING; RNA TRANSPORT;

BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELL ADHESION MOLECULES; CELL NUCLEUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXONS; GENES, REPORTER; HELA CELLS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INTRONS; MODELS, BIOLOGICAL; NUCLEAR PROTEINS; PLASMIDS; PRECIPITIN TESTS; PROTEIN BINDING; RIBONUCLEASE H, CALF THYMUS; RIBONUCLEOPROTEINS; RNA SPLICING; RNA, MESSENGER; TRANSFECTION;

EID: 0141752774     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.23.20.7363-7376.2003     Document Type: Article

References (49)

1. Aebi, U., J. Cohn, L. Buhle, and L. Gerace. 1986. The nuclear lamina is a meshwork of intermediate-type filaments. Nature 323:560-564.
2. Blencowe, B. J., R. Issner, J. A. Nickerson, and S. A. Sharp. 1998. A coactivator of pre-mRNA splicing. Genes Dev. 12:996-1009.
3. Brandner, J. M., S. Reidenbach, and W. W. Franke. 1997. Evidence that "pnn," reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein. Differentiation 62:119-127.
4. Brandner, J. M., S. Reidenbach, C. Kuhn, and W. W. Franke. 1998. Identification and characterization of a novel kind of nuclear protein occurring free in the nucleoplasm and in ribonucleoprotein structures of the "speckle" type. Eur. J. Cell Biol. 75:295-308.
5. Brodsky, A. S., and P. A. Silver. 2000. Pre-mRNA processing factors are required for nuclear export. RNA 6:1737-1749.
6. Dellaire, G., E. M. Makarov, J. J. M. Cowger, D. Longman, H. G. E. Sutherland, R. Luhrmann, J. Torchia, and W. A. Bickmore. 2002. Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes. Mol. Cell. Biol. 22:5141-5156.
7. Dignam, J. D., R. M. Lebovitz, and R. G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475-1489.
8. Dostie, J., and G. Dreyfuss. 2002. Translation is required to remove Y14 from mRNAs in the cytoplasm. Curr. Biol. 12:1060-1067.
9. Fu, X., A. Mayeda, T. Maniatis, and A. R. Krainer. 1992. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5′ and 3′ splice site selection. Proc. Natl. Acad. Sci. USA 89:11224-11228.
10. Gallouzi, I. E., and J. A. Steitz. 2001. Delineation of mRNA export pathways by the use of cell-permeable peptides. Science 294:1895-1901.
11. Gatfield, D., and E. Izaurralde. 2002. REF1/Aly and the additional exon junction complex proteins are dispensable for nuclear mRNA export. J. Cell Biol. 159:579-588.
12. Gehring, N. H., G. Neu-Yilik, T. Schell, M. W. Hentze, and A. E. Kulozik. 2003. Y14 and hUpf3b form an NMD-activating complex. Mol. Cell 11:939-949.
13. Graveley, B. R. 2000. Sorting out the complexity of SR protein functions. RNA 6:1197-1211.
14. Hachet, O., and A. Ephrussi. 2001. Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport. Curr. Biol. 11:1666-1674.
15. Hentze, M. W., and A. E. Kulozik. 1999. A perfect message: RNA surveillance and nonsense-mediated decay. Cell 96:307-310.
16. Herold, A., T. Klymenko, and E. Izaurralde. 2001. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA 7:1768-1780.
17. Huang, Y., R. Gattoni, J. Stevenin, and J. A. Steitz. 2003. SR splicing factors serve as adapter proteins for TAP-dependent mRNA export. Mol. Cell 11:837-843.
18. Islas, S., J. Vega, L. Ponce, and L. Gonzalea-Mariscal. 2002. Nuclear localization of the tight junction protein ZO-2 in epithelial cells. Exp. Cell. Res. 274:138-148.
19. Jurica, M. S., L. J. Licklider, S. R. Gygi, N. Grigorieff, and M. J. Moore. 2002. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA 8:426-439.
20. Kataoka, N., J. Yong, V. N. Kim, F. Velazquez, R. A. Perkinson, F. Wang, and G. Dreyfuss. 2000. Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm. Mol. Cell 6:673-682.
21. Kim, V. N., N. Kataoka, and G. Dreyfuss. 2001. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science 293:1832-1836.
22. Kramer, A. 1996. The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem. 65:367-409.
23. Lai, M. C., B. H. Teh, and W. Y. Tarn. 1999. A human papillomavirus E2 transcriptional activator. The interactions with cellular splicing factors and potential function in pre-mRNA processing. J. Biol. Chem. 274:11832-11841.
24. Le Hir, H., M. J. Moore, and L. E. Maquat. 2000. Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junction. Genes Dev. 14:1098-1108.
25. Le Hir, H., E. Izaurralde, L. E. Maquat, and M. J. Moore. 2000. The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. EMBO J. 19:6860-6869.
26. Le Hir, H., D. Gatfield, E. Izaurralde, and M. J. Moore. 2001. The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated decay. EMBO J. 20:4987-4997.
27. Lu, S., and B. R. Cullen. 2003. Analysis of the stimulatory effect of splicing on mRNA production and utilization in mammalian cells. RNA 9:618-630.
28. Luo, M., and R. Reed. 1999. Splicing is required for rapid and efficient mRNA export in metazoans. Proc. Natl. Acad. Sci. USA 96:14937-14942.
29. Lykke-Andersen, J., M. D. Shu, and J. A. Steitz. 2001. Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1. Science 293:1836-1839.
30. McCracken, S., M. Lambermon, and B. J. Blencowe. 2002. SRm160 splicing coactivator promotes transcript 3′-end cleavage. Mol. Cell. Biol. 22:148-160.
31. Matsumoto, K., K. M. Wassarman, and A. P. Wolffe. 1998. Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA. EMBO J. 17:2107-2121.
32. Mayeda, A., J. Badolato, R. Kobayashi, M. Q. Zhang, E. M., Gardiner, and A. R. Krainer. 1999. Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. EMBO J. 16:4560-4570.
33. Micklem, D. R., R. Dasgupta, H. Elliott, F. Gergely, C. Davidson, A. Brand, A. Gonzalez-Reyes, and D. St. Johnston. 1997. The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila. Curr. Biol. 7:468-478.
34. Mohr, S. E., S. T. Dillon, and R. Boswell. 2001. The RNA-binding protein Tsunagi interacts with Mago Nashi to establish polarity and localize oskar mRNA during Drosophila oogenesis. Genes Dev. 15:2886-2899.
35. Nott, A., S. H. Meislin, and M. J. Moore. 2003. A quantitative analysis of intron effects on mammalian gene expression. RNA 9:607-617.
36. Ohno, M., A. Segref, S. Kuersten, and I. W. Mattaj. 2002. Identity elements used in export of mRNAs. Mol. Cell 9:659-671.
37. Ouyang, P., and S. P. Sugrue. 1992. Identification of an epithelial protein related to the desmosome and intermediate filament network. J. Cell Biol. 118:1477-1488.
38. Ouyang, P., and S. P. Sugrue. 1996. Characterization of Pinin, a novel protein associated with the desmosome-intermediate filament complex. J. Cell Biol. 135:1027-1042.
39. Ouyang, P. 1999. Antibodies differentiate desmosome-form and nucleus-form Pinin: evidence that Pinin is a moonlighting protein with dual location at the desmosome and within the nucleus. Biochem. Biophys. Res. Commun. 263:192-200.
40. Reed, R., and E. Hurt. 2002. A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell 108:523-531.
41. Rodrigues, J. P., M. Rode, D. Gatfield, B. J. Blencowe, M. Carmo-Fonseca, and E. Izaurralde. 2001. REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus. Proc. Natl. Acad. Sci. USA 98:1030-1035.
42. Schwerk, C., J. Prasad, K. Degenhardt, H. Erdjument-Bromage, E. White, P. Tempst, V. J. Kidd, J. L. Manley, J. M. Lahti, and D. Reinberg. 2003. ASAP, a novel protein complex involved in RNA processing and apoptosis. Mol. Cell. Biol. 23:2981-2990.
43. Shi, Y., P. Ouyang, and S. P. Sugrue. 2000. Characterization of the gene encoding pnn/DRS/memA and evidence for its potential tumor suppressor function. Oncogene 19:289-297.
44. cip1/waf1. Oncogene 20:4007-4018.
45. Takagaki, Y., and J. L. Manley. 2000. Complex protein interactions within the human polyadenylation machinery identify a novel component. Mol. Cell. Biol. 20:1515-1525.
46. Tarn, W. Y., and J. A. Steitz. 1994. SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes Dev. 8:2704-2717.
47. Wang, P., P. J. Lou, S. Leu, and P. Ouyang. 2002. Modulation of alternative pre-mRNA splicing in vivo by Pinin. Biochem. Biophy. Res. Commun. 294:448-455.
48. Zahler, A. M., W. S. Lane, J. A. Stolk, and M. B. Roth. 1992. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6:837-847.
49. Zhou, Z., M. J. Luo, K. Straesser, J. Katahira, E. Hurt, and R. Reed. 2000. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. Nature 407:401-405.