Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis

RNA

Volumn 8, Issue 4, 2002, Pages 426-439

  Jurica, Melissa S ^a   Licklider, Lawrence J ^a   Gygi, Steven P ^a   Grigorieff, Nikolaus ^a   Moore, Melissa J ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

Electron microscopy; Mass spectroscopy; Pre mRNA splicing; Spliceosomes

Indexed keywords

MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA; SPLICEOSOME ASSOCIATED PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; ELECTRON MICROSCOPY; IMAGE PROCESSING; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RNA SPLICING; SEQUENCE ANALYSIS; SPLICEOSOME; STAINING; STRUCTURE ANALYSIS; TANDEM MASS SPECTROMETRY; UNINDEXED SEQUENCE;

ADENOSINE TRIPHOSPHATASES; CELL CYCLE PROTEINS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; MASS SPECTROMETRY; MICROSCOPY, ELECTRON; RIBONUCLEASE H, CALF THYMUS; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA, SMALL NUCLEAR; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SPLICEOSOMES;

EID: 0036242097     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838202021088     Document Type: Article

References (71)

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17. Evidence for a linear search in bimolecular 3′ splice site AG selection
18. PRP19: A novel spliceosomal component
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31. A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor
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34. The Y14 protein communicates to the cytoplasm the position of exonexon junctions
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38. The protein Mago provides a link between splicing and mRNA localization
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40. The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions
41. Mutants of the bacteriophage MS2 coat protein that alter its cooperative binding to RNA
42. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes
43. The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes
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50. Proteomics: The move to mixtures
51. Crystal structure of the spliceosomal U2B″-U2A′ protein complex bound to a fragment of U2 small nuclear RNA
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53. Purification and visualization of native spliceosomes
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55. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells
56. Ximdisp - A visualization tool to aid structure determination from electron microscope images
57. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly
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61. Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins
62. The second catalytic step of pre-mRNA splicing
63. A new generation of IMAGIC image processing system
64. Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment
65. Structure of the 30S ribosomal subunit
66. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing
67. Crystal structure of the ribosome at 5.5 Å resolution
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