Human recombinant anti-La (SS-B) autoantibodies demonstrate the accumulation of phosphoserine-366-containing La isoforms in nucleoplasmic speckles

European Journal of Cell Biology

Volumn 82, Issue 3, 2003, Pages 131-141

  Raats, Jos M H ^a   Roeffen, Will F ^a   Litjens, Sandy ^a   Bulduk, Ibrahim ^a   Mans, Gijs ^a   van Venrooij, Walther J ^a   Pruijn, Ger J M ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Apoptosis; La(SS B); Phosphorylation; Recombinant antiboby fragments; Systemic lupus erythematosus (SLE)

Indexed keywords

AUTOANTIBODY; CELL EXTRACT; DNA DIRECTED RNA POLYMERASE III; ISOPROTEIN; LA ANTIBODY; PHOSPHOSERINE; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; RIBONUCLEOPROTEIN; RIBONUCLEOPROTEIN ANTIBODY; SERINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIGEN BINDING; ANTIGEN RECOGNITION; APOPTOSIS; CELLULAR DISTRIBUTION; COILED BODY; COMBINATORIAL LIBRARY; CONTROLLED STUDY; GERM LINE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOREACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REVIEW; SEQUENCE ANALYSIS; SPLICEOSOME; SYSTEMIC LUPUS ERYTHEMATOSUS; SYSTEMIC SCLEROSIS; WESTERN BLOTTING;

ANIMALIA;

EID: 0037354554     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1078/0171-9335-00304     Document Type: Review

References (34)

1. H gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells. J. Clin. Invest. 99, 2488-2501.
2. Broekhuis, C. H., Neubauer, G., van der Heijden, A., Mann, M., Proud, C. G., van Venrooij, W. J., Pruijn, G. J. (2000): Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. Biochemistry 39, 3023-3033.
3. Casciola-Rosen, L. A., Miller, D. K., Anhalt, G. J., Rosen, A. (1994): Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death. J. Biol. Chem. 269, 30757-30760.
4. Clackson, T., Hoogenboom, H. R., Griffiths, A. D., Winter, G. (1991): Making antibody fragments using phage display libraries. Nature 352, 624-628.
5. De Bellis, D., Schwartz, I. (1990): Regulated expression of foreign genes fused to lac: control by glucose levels in growth medium. Nucleic Acids Res. 18, 1311.
6. De Wildt, R. M., Finnern, R., Ouwehand, W. H., Griffiths, A. D., van Venrooij, W. J., Hoet, R. M. A. (1996): Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and patient-derived combinatorial V gene library. Eur. J. Immunol. 26, 629-639.
7. De Wildt, R. M., Tomlinson, I. M., van Venrooij, W. J., Winter, G., Hoet, R. M. A. (2000): Comparable heavy and light chain pairings in normal and systemic lupus erythematosus IgG(+) B cells. Eur. J. Immunol. 30, 254-261.
8. Degen, W. G. J., Pieffers, M., Welin-Henriksson, E., van Venrooij, W. J., Raats, J. M. H. (2000): Characterization of recombinant human autoantibody fragments directed toward the autoantigenic U1-70K protein. Eur. J. Immunol. 30, 3029-3038.
9. Dörner, T., Farner, N. L., Lipsky, P. E. (1999): Ig λ and heavy chain gene usage in early untreated systemic lupus erythematosus suggests intensive B cell stimulation. J. Immunol. 163, 1027-1036.
10. Dörner, T., Foster, S. J., Farner, N. L., Lipsky, P. E. (1998): Immunoglobulin κ chain receptor editing in systemic lupus erythematosus. J. Clin. Invest. 102, 688-694.
11. Fan, H., Sakulich, A. L., Goodier, J. L., Zhang, X. L., Qin, J., Maraia, R. J. (1997): Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexes. Cell 88, 707-715.
12. Finnern, R., Pedrollo, E., Fisch, I., Wieslander, J., Marks, J. D., Lockwood, C. M., Ouwehand, W. H. (1997): Human autoimmune antiproteinase 3 scFv from a phage display library. Clin. Exp. Immunol. 107, 269-281.
13. Fouraux, M. A., Kolkman, M. J. M., van der Heijden, A., de Jong, A. S., van Venrooij W. J., Pruijn, G. J. (2002): The Human La (SS-B) autoantigen interacts with DDX15, a putative DEAH-box RNA helicase. RNA 8, 1428-1443.
14. Griffiths, A. D., Williams, S. C., Hartley, O., Tomlinson, I. M., Waterhouse, P., Crosby, W. L., Kontermann, R. E., Jones, P. T., Low, N. M., Allison, T. J., Prospero, T. D., Hoogenboom, H. R., Nissim, A., Cox, J. P. L., Harrison, J. L., Zaccolo, M., Gherardi, E., Winter, G. (1994): Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
15. Habets, W. J., Hoet, M. H., De Jong, B. A., Van der Kemp, A., van Venrooij, W. J. (1989): Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies. J. Immunol. 143, 2560-2566.
16. Harlow, E., Lane, D. (1988): Antibodies - A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Hoet, R. M. A., Raats, J. M. H., de Wildt, R., Dumortier, H., Muller, S., van den Hoogen, F., van Venrooij, W. J. (1998): Human monoclonal antibody fragments from combinatorial antibody libraries directed to the U1snRNP associated U1C protein; epitope mapping, immunolocalization and V-gene usage. Mol. Immunol. 35, 1045-1055.
18. Jones, E., Kimura, H., Vigneron, M., Wang, Z. X., Roeder, R. G., Cook, P. R. (2000): Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III. Exp. Cell Res. 254, 163-172.
19. Kiss, T. (2001): Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs. EMBO J. 20, 3617-3622.
20. Kufel, J., Allmang, C., Chanfreau, G., Petfalski, E., Lafontaine, D. L., Tollervey, D. (2000): Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding. Mol. Cell. Biol. 20, 5415-5424.
21. Lerner, E. A., Lerner, M. R., Janeway, C. A., Jr., Steitz, J. A. (1981): Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc. Natl. Acad. Sci. USA 78, 2737-2741.
22. Maraia, R. J., Intine, R. V. (2001): Recognition of nascent RNA by the human La antigen: Conserved and diverged features of structure and function. Mol. Cell. Biol. 21, 367-379.
23. Matera, A. G. (1998): Of coiled bodies, gems, and salmon. J. Cell. Biochem. 70, 181-192.
24. Matera, A. G., Frey, M. R. (1998): Coiled bodies and gems: Janus or gemini? Am. J. Hum. Genet. 63, 317-321.
25. Pruijn, G. J. M., Thijssen, J. P., Smith, P. R., Williams, D. G., van Venrooij W. J. (1995): Anti-La monoclonal antibodies recognizing epitopes within the RNA-binding domain of the La protein show differential capacities to immunoprecipitate RNA-associated La protein. Eur. J. Biochem. 232, 611-619.
26. Raats, J. M. H., van Bree, N., van Woezik, J., Pruijn, G. J. M. (2003): Generating recombinant antibodies for the detection of haptens in solution. J. Immunoassay Immunochem. In press.
27. Rutjes, S. A., Utz, P. J., Broekhuis, C., van der Heijden, A., van Venrooij, W. J., Pruijn, G. J. M. (1999): The La (SS-B) autoantigen, a key protein in RNA biogenesis, is dephosphorylated and cleaved early during apoptosis. Cell Death Differ. 6, 976-986.
28. Sleeman, J. E., Ajuh, P., Lamond, A. I. (2001): snRNP protein expression enhances the formation of Cajal bodies containing p80-coilin and SMN. J. Cell Sci. 114, 4407-4419.
29. H segments with different hypervariable loops. J. Mol. Biol. 227, 776-798.
30. Tran, H. B., Ohlsson, M., Beroukas, D., Hiscock, J., Bradley, J., Buyon, J. P., Gordon, T. P. (2002): Subcellular redistribution of La/SSB autoantigen during physiologic apoptosis in the fetal mouse heart and conduction system. A clue to the pathogenesis of congenital heart-block. Arthritis Rheum. 46, 202-208.
31. Tzioufas, A. G., Yiannaki, E., Sakarellos Daitsiotis, M., Routsias, J. G., Sakarellos, C., Moutsopoulos, H. M. (1997): Fine specificity of autoantibodies to La/SSB: Epitope mapping, and characterization. Clin. Exp. Immunol. 108, 191-198.
32. Utz, P. J., Anderson, P. (1998): Posttranslational protein modifications, apoptosis, and the bypass of tolerance to autoantigens. Arthritis Rheum. 41, 1152-1160.
33. Wolin, S. L., Cedervall, T. (2002): The La protein. Annu. Rev. Biochem. 71, 375-403.
34. Xue, D., Rubinson, D. A., Pannone, B. K., Yoo, C. J., Wolin, S. L. (2000): UsnRNP assembly in yeast involves the La protein. EMBO J. 19, 1650-1660.