Fine specificity of autoantibodies to La/SSB: Epitope mapping, and characterization

Clinical and Experimental Immunology

Volumn 108, Issue 2, 1997, Pages 191-198

  Tzioufas, A G ^a   Yiannaki, E ^b   Sakarellos Daitsiotis, M ^b   Routsias, J G ^b   Sakarellos, C ^b   Moutsopoulos, H M ^a,c  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF IOANNINA   (Greece)

^c UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

B cell epitopes; La SSB; molecular mimicry; Sjogren's syndrome; systemic lupus erythematosus

Indexed keywords

AUTOANTIBODY; DNA TOPOISOMERASE (ATP HYDROLYSING); EPITOPE; MYELIN BASIC PROTEIN; RIBONUCLEOPROTEIN ANTIBODY; SYNTHETIC PEPTIDE;

ADULT; AGED; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; EPITOPE MAPPING; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; MOLECULAR MIMICRY; PRIORITY JOURNAL; SJOEGREN SYNDROME; SYSTEMIC LUPUS ERYTHEMATOSUS;

EID: 0030992337     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.1997.d01-1003.x     Document Type: Article

References (39)

1. Pruijn CJM, Slobbe RL, van Venrooij WJ. Structure and function of La and Ro RNPs. Mol Biol Rep 1990; 14:43-48.
2. Chan EKL, Francoeur AM, Tan EM. Epitopes, structural domains and asymmetry of amino acid residues in SSB/La nuclear protein. J Immunol 1986; 136:3744-9.
3. Chan EKL, Tan EM. The small nuclear ribonucleoprotein SSB/La binds RNA with a conserved protease-resistant domain of 28 kilodaltons. Mol Cell Biol 1987; 7:2588-94.
4. Sturgess AD, Peterson MG, McNeilage LJ, Whittingham S, Coppel RL. Characteristics and epitope mapping of a cloned human autoantigen La. J Immunol 1988; 140:3212-8.
5. Rauh AJG, Lührmann R. At least 3 distinct B cell epitopes reside in the C-terminal half of La protein, as determined by a recombinant DNA approach. Eur J Immunol 1988; 18:2049-57.
6. St Clair EW, Pisetsky DS, Reich CF, Keene JD. Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins. J Immunol 1988; 141:4173-80.
7. Bini P, Chu J-L, Okolo C, Elkon K. Analysis of autoantibodies to recombinant La(SSB) peptides in systemic lupus erythematosus and primary Sjögren's syndrome. J Clin Invest 1990; 85:325-33.
8. Tan EM, Cohen AS, Fries JB et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25:1271-7.
9. Vitali C, Bombardieri S, Moutsopoulos HM et al. Preliminary criteria for the classification of Sjögren's syndrome. Arthritis Rheum 1993; 36:340-7.
10. Geysen HM, Rodda SJ, Mason TJ, Tribbick G, Schoofs PG. Strategies for epitope analysis using peptide synthesis. J Immunol Methods 1987; 202:259.
11. Merrifield RB. Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. JACS 1963; 85:2149-54.
12. Cohen SA, Stydous DJ. Aminoacid analysis utilising phenylsothyiocynate derivatives. Anal Biochem 1988; 174:1-16.
13. Stewart JM, Young JD, eds. Solid phase peptide synthesis. Rockford: Pierce Chemical Co., 1984.
14. Habets WJ, De Rooij DJ, Hoet MH, Van de Putte LB, van Venrooij WJ. Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunobloting. Clin Exp Immunol 1985, 59:457-66.
15. Mendez-Arias L, Rodriguez RA. BASIC microcomputer program for prediction of B and T cell epitopes in proteins. CABIOS 1990; 6:101-5.
16. Burkhard R, Sander C. Prediction of protein secondary structure at better that 70% accuracy. J Mol Biol 1993; 232:584-99.
17. Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 1988; 85:2444-8.
18. Smith TF, Waterman MS. Identification of common molecular subsequences. J Mol Biol 1988; 147:195-7.
19. Altschul SE. Amino acid substitution matrices from an information theoretic perspective. J Mol Biol 1991; 219:555-65.
20. Chambers JC, Kenan D, Martin BJ, Keene JD. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem 1988; 263:18043-51.
21. Chan EKL, Sullivan KF, Tan EM. Ribonucleoprotein SSB/La belongs to a protein family with consensus sequences for RNA binding. Nucleic Acid Res 1988; 17:2233-44.
22. Kohsaka H, Yamamoto K, Fujii H et al. Fine specificity of the human SSB/La protein: identification of a distinct autoepitope homologous to a viral gag polyprotein. J Clin Invest 1990; 85:1566-74.
23. Welling GM, Weijer WJ, Van der Zee R, Welling-Wester. Prediction of sequential antigenic regions in proteins. FEBS Letters 1985; 188:215-8.
24. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci USA 1981; 78:3824-8.
25. Karplus PA, Schultz GE. Prediction of chain flexibility in proteins. Naturwissenschaften, 1985; 72:212-3.
26. Margalit H, Spouge JL, Cornette JL, Cease KB, Delisi C, Berzofsky JA. Prediction of immunodominant helper T cell antigenic sites from the primary sequences. J Immunol 1987; 138:2213-29.
27. Rothbard JB, Taylor WR. A sequence pattern comment to T cell epitopes. EMBO J 1988; 7:93-100.
28. Sette A, Buus S, Appela E et al. Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis. Proc Natl Acad Sci USA 1989; 86:3296-300.
29. Routsias JG, Tzioufas AG, Sakarellos-Daitsiotis M, Sakarellos C, Moutsopoulos HM. Epitope mapping of the Ro/SSA 60KD autoantigen reveals disease specific antibody binding profiles. Eur J Clin Invest 1996 26:514-21.
30. Scofield RH, Harley JB. Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus. Proc Natl Acad Sci USA 1991; 88:3343-7.
31. Tsikaris V, Vlachoyiannopoulos PG, Panou E et al. Immunoreactivity and conformation of the P-P-G-M-R-P-P repetitive epitope of the Sm autoantigen. Int J Pep Prot Res 1996; 48:319-27.
32. St Clair EN, Burch JA, Ward MM, Keene JD, Pisetsky DS. Temporal correlation of antibody responses to different epitopes of the human La autoantigen. J Clin Invest 1990; 85:515-21.
33. Van Regenmortel MHV. Protein antigenicity. Mol Biol Reports 1992; 16:133-8.
34. Rischmueller M, Mc Neilage LJ, McCluskey J, Gordon T. Human autoantibodies directed against the RNA recognition motif of La(SSB) bind to conformational epitope present on the intact La(SSB)/Ro(SSA) ribonucleoprotein particle. Clin Exp Immunol 1995;101:39-44.
35. Topfer F, Gordon T, McCluskey J. Intra- and inter-molecular spreading of autoimmunity involving the nuclear self-antigens La(SSB) and Ro(SSA). Proc Natl Acad Sci USA 1995; 92:875-9.
36. Miller A, Al-Sabbagh A, Santos LMB, Pradhu-Das M, Weiner HL. Epitopes of myelin basic protein (MBP) that trigger TGF- β release following oral tolerization to MBP are different from encephalitogenic epitopes and mediate epitope-driven bystander suppression. J Immunol 1993; 151:7307-15.
37. Meliconi R, Bestagno M, Sturani C et al. Autoantibodies to DNA topoisomerase II in cryptogenic fibrosing alveolitis and connective tissue disease. Clin Exp Immunol 1989; 76:184-9.
38. Owe-Young RA, Horn S, Edmonds JP, Sturgess AD. Use of molecularly cloned human SSB antigen to detect anti SSB antibodies. J Autoimmun 1992; 5:351-61.
39. Tsikaris V, Sakarellos C, Cung-Thong M, Marraud M, Sakarellos-Daitsiotis M. Concept and design of a new class of sequential oligopeptide carriers (SOC) for covalent attachment of multiple antigenic peptides. Biopolymer, 1996; 38:291-3.