Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III

Experimental Cell Research

Volumn 254, Issue 1, 2000, Pages 163-172

  Jones, Emma ^a   Kimura, Hiroshi ^a   Vigneron, Marc ^b   Wang, Zhengxin ^c   Roeder, Robert G ^c   Cook, Peter R ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b INSERM   (France)

^c ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Monoclonal antibody; Nuclear structure; RPB6; RPB8; RPC32; RPC39; Sm antigen

Indexed keywords

5,6 DICHLOROBENZIMIDAZOLE RIBOSIDE; AUTOANTIGEN; DACTINOMYCIN; DNA DIRECTED RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE III; MONOCLONAL ANTIBODY; RNA POLYMERASE II;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL NUCLEUS; ENZYME LOCALIZATION; ENZYME SUBUNIT; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCHEMISTRY; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA;

EID: 0034627776     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4739     Document Type: Article

References (73)

1. Chambon P. RNA polymerases. Boyer P. D. The Enzymes. 1974;261-331 Academic Press, New York.
2. Weinmann R., Jaehning J. A., Raskas H. J., Roeder R. G. Viral RNA synthesis and levels of DNA-dependent RNA polymerases during replication of adenovirus 2. J. Virol. 17:1975;114-126.
3. Young R. A. RNA polymerase II. Annu. Rev. Biochem. 60:1991;689-715.
4. Sentenac A., Riva M., Thuriaux P., Buhler J. M., Treich I., Carles M. W., Ruet A., Huet J., Mann C., Chiannilkulchai N., Stettler S., Mariotte S. Yeast RNA polymerase subunits and genes. Transcriptional Regulation. 1992;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
5. Shpakovski G. V., Acker J., Wintzerith M., Lacroix J. F., Thuriaux P., Vigneron M. Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae. Mol. Cell. Biol. 15:1995;4702-4710.
6. Khazak V., Estojak J., Cho H., Majors J., Sonoda G., Testa J. R., Golemis E. A. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Mol. Cell. Biol. 18:1998;1935-1945.
7. Song C. Z., Hanada K., Yano K., Maeda Y., Yamamoto K., Muramatsu M. High conservation of subunit composition of RNA polymerase I(A) between yeast and mouse and the molecular cloning of mouse RNA polymerase I 40-kDa subunit RPA40. J. Biol. Chem. 269:1994;26976-26981.
8. Hannan R. D., Hempel W. M., Cavanaugh A., Arino T., Dimitrov S. I., Moss T., Rothblum L. Affinity purification of mammalian RNA polymerase I. Identification of an associated kinase. J. Biol. Chem. 273:1998;1257-1267.
9. Seither P., Iben S., Grummt I. Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors. J. Mol. Biol. 275:1998;43-53.
10. Seither P., Grummt I. Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I. Genomics. 37:1996;135-139.
11. Yao Y., Yamamoto K., Nishi Y., Nogi Y., Muramatsu M. Mouse RNA polymerase I 16-kDa subunit able to associate with 40-kDa subunit is a homolog of yeast AC19 subunit of RNA polymerases I and III. J. Biol. Chem. 271:1996;32881-32885.
12. Seither P., Coy J. F., Pouska A., Grummt I. Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I. Mol. Gen. Genet. 255:1997;180-186.
13. Wang Z., Luo T., Roeder R. G. Identification of an autonomously initiating RNA polymerase III holoenzyme containing a novel factor that is selectively inactivated during protein synthesis inhibition. Genes Dev. 11:1997;2371-2382.
14. Ittmann M., Ali J., Greco A., Basilico C. The gene complementing a temperature-sensitive cell cycle mutant of BHK cells is the human homologue of the yeast RPC53 gene, which encodes a subunit of RNA polymerase C (III). Cell Growth Differ. 4:1993;503-511.
15. Wang Z., Roeder R. G. Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation. Genes Dev. 11:1997;1315-1326.
16. Chedin S., Riva M., Schultz P., Sentenac A., Carles C. The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination. Genes Dev. 12:1998;3857-3871.
17. Memet S., Saurin W., Sentenac A. RNA polymerases B and C are more closely related to each other than to RNA polymerase A. J. Biol. Chem. 263:1988;10048-10051.
18. McKune K., Moore P. A., Hull M. W., Woychik N. A. Six human RNA polymerase subunits functionally substitute for their yeast counterparts. Mol. Cell. Biol. 15:1995;6895-6900.
19. Acker J., de Graaff M., Cheynel I., Khazak V., Kedinger C., Vigneron M. Interactions between the human RNA polymerase II subunits. J. Biol. Chem. 272:1997;16815-16821.
20. Scheer U., Rose K. M. Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry. Proc. Natl. Acad. Sci. USA. 81:1984;1431-1435.
21. Weisenberger D., Scheer U. A possible mechanism for the inhibition of ribosomal RNA gene transcription during mitosis. J. Cell Biol. 129:1995;561-575.
22. Jordan P., Mannervik M., Tora L., Carmo Fonseca M. In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive. J. Cell Biol. 133:1996;225-234.
23. Roussel P., Andre C., Comai L., Hernandez-Verdun D. The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs. J. Cell Biol. 133:1996;235-246.
24. Thibodeau A., Vincent M. Monoclonal antibody CC-3 recognizes phosphoproteins in interphase and mitotic cells. Exp. Cell Res. 195:1991;145-153.
25. Wansink D. G., Schul W., van der Kraan I., van Steensel B., van Driel R., de Jong L. Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J. Cell Biol. 122:1993;283-293.
26. Bregman D. B., Du L., Li Y., Ribisi S., Warren S. L. Cytostellin distributes to nuclear regions enriched with splicing factors. J. Cell Sci. 107:1994;387-396.
27. Bregman D. B., Du L., van der Zee S., Warren S. L. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J. Cell Biol. 129:1995;287-298.
28. Iborra F. J., Pombo A., Jackson D. A., Cook P. R. Active RNA polymerases are localized within discrete transcription "factories" in human nuclei. J. Cell Sci. 109:1996;1427-1436.
29. Mortillaro M. J., Blencowe B. J., Wei X., Nakayasu H., Du L., Warren S. L., Sharp P. A., Berezney R. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc. Natl. Acad. Sci. USA. 93:1996;8253-8257.
30. Nguyen V. T., Giannoni F., Dubois M. F., Seo S. J., Vigneron M., Kedinger C., Bensaude O. In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin. Nucleic Acids Res. 24:1996;2924-2929.
31. Kimura M., Ishiguro A., Ishihama A. RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity. J. Biol. Chem. 272:1997;25851-25855.
32. Langer D., Hain J., Thuriaux P., Zillig W. Transcription in archaea: similarity to that in eucarya. Proc. Natl. Acad. Sci. USA. 92:1995;5768-5772.
33. Archambault J., Schappert K. T., Friesen J. D. A suppressor of an RNA polymerase II mutation of Saccharomyces cerevisiae encodes a subunit common to RNA polymerases I, II, and III. Mol. Cell. Biol. 10:1990;6123-6131.
34. Nouraini S., Archambault J., Friesen J. D. Rpo26p, a subunit common to yeast RNA polymerases, is essential for the assembly of RNA polymerases I and II and for the stability of the largest subunits of these enzymes. Mol. Cell. Biol. 16:1996;5985-5996.
35. Lanzendorfer M., Smid A., Klinger C., Schultz P., Sentenac A., Carles C., Riva M. A shared subunit belongs to the eukaryotic core RNA polymerase. Genes Dev. 11:1997;1037-1047.
36. Buhler J. M., Huet J., Davies K. E., Sentenac A., Fromageot P. Immunological studies of yeast nuclear RNA polymerases at the subunit level. J. Biol. Chem. 255:1980;9949-9954.
37. Breant B., Huet J., Sentenac A., Fromageot P. Analysis of yeast RNA polymerases with subunit-specific antibodies. J. Biol. Chem. 258:1983;11968-11973.
38. Huet J., Riva M., Sentenac A., Fromageot P. Yeast RNA polymerase C and its subunits. Specific antibodies as structural and functional probes. J. Biol. Chem. 260:1985;15304-15310.
39. Bult C. J., White O., Olsen G. J., Zhou L., Fleischmann R. D., Sutton G. G., Blake J. A., FitzGerald L. M., Clayton R. A., Gocayne J. D., Kerlavage A. R., Dougherty B. A., Tomb J. F., Adams M. D., Reich C. I., Overbeek R., Kirkness E. F., Weinstock K. G., Merrick J. M., Glodek A., Scott J. L., Geoghagen N. S. M., Venter J. C. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science. 273:1996;1058-1073.
40. Woychik N. A., Liao S. M., Kolodziej P. A., Young R. A. Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev. 4:1990;313-323.
41. Krapp S., Kelly G., Reischl J., Weinzierl R. O., Matthews S. Eukaryotic RNA polymerase subunit RPB8 is a new relative of the OB family. Nat. Struct. Biol. 5:1998;110-114.
42. Mosrin C., Riva M., Beltrame M., Cassar E., Sentenac A., Thuriaux P. The RPC31 gene of Saccharomyces cerevisiae encodes a subunit of RNA polymerase C (III) with an acidic tail. Mol. Cell. Biol. 10:1990;4737-4743.
43. Thuillier V., Stettler S., Sentenac A., Thuriaux P., Werner M. A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation. EMBO J. 14:1995;351-359.
44. Werner M., Hermann Le Denmat S., Treich I., Sentenac A., Thuriaux P. Effect of mutations in a zinc-binding domain of yeast RNA polymerase C (III) on enzyme function and subunit association. Mol. Cell. Biol. 12:1992;1087-1095.
45. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
46. Harlow E., Lane D. Antibodies: A Laboratory Manual. 1988;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
47. Hoffmann A., Roeder R. G. Cloning and characterization of human TAF20/15. Multiple interactions suggest a central role in TFIID complex formation. J. Biol. Chem. 271:1996;18194-18202.
48. Jackson S. P. Hames B. M., Higgins S. J. Gene Transcription - A Practical Approach. 1992;206-210 IRL Press, Oxford.
49. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
50. Schneider C., Newman R. A., Sutherland D. R., Asser U., Greaves M. F. A one-step purification of membrane proteins using a high efficiency immunomatrix. J. Biol. Chem. 257:1982;10766-10769.
51. Thompson N. E., Steinberg T. H., Aronson D. B., Burgess R. R. Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264:1989;11511-11520.
52. Besse S., Vigneron M., Pichard E., Puvion-Dutilleul F. Synthesis and maturation of viral transcripts in herpes simplex virus type 1 infected HeLa cells: the role of interchromatin granules. Gene Exp. 4:1995;143-161.
53. Tan E. M., Fritzler M. J., McDougal J. S., McDuffie F. C., Nakamura R. M., Reichlin M., Reimer C. B., Sharp G. C., Schur P. H., Wilson M. R., Winchester R. J. Reference sera for antinuclear antibodies. I. Antibodies to native DNA, Sm, nuclear RNP, and SS-B/La. Arthritis Rheum. 25:1982;1003-1005.
54. Acker J., Wintzerith M., Vigneron M., Kedinger C. Interactions between the human RNA polymerase II subunits. DNA Seq. 4:1994;329-331.
55. Carmo-Fonseca M., Ferreira J., Lamond A. I. Assembly of sn RNP-containing coiled bodies is regulated in interphase and mitosis - Evidence that the coiled body is a kinetic nuclear structure. J. Cell Biol. 120:1993;841-852.
56. Spector D. L. Nuclear organization and gene expression. Exp. Cell Res. 229:1996;189-197.
57. Zandomeni R., Zandomeni M. C., Shugar D., Weinmann R. Casein kinase type II is involved in the inhibition by 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription. J. Biol. Chem. 261:1986;3414-3419.
58. Dubois M. F., Bellier S., Seo S. J., Bensaude O. Phosphorylation of the RNA polymerase II largest subunit during heat shock and inhibition of transcription in HeLa cells. J. Cell Physiol. 158:1994;417-426.
59. Tamm I., Hand R., Caliguiri L. A. Action of dichlorobenzimidazole riboside on RNA synthesis in L-929 and HeLa cells. J. Cell Biol. 69:1976;229-240.
60. Zandomeni R., Mittleman B., Bunick D., Ackerman S., Weinmann R. Mechanism of action of dichloro-β-D-ribofuranosylbenzimidazole: Effect on in vitro transcription. Proc. Natl. Acad. Sci. USA. 79:1982;3167-3170.
61. Scheer U., Hugle B., Hazan R., Rose K. M. Drug-induced dispersal of transcribed rRNA genes and transcriptional products: immunolocalization and silver staining of different nucleolar components in rat cells treated with 5,6-dichloro-β-D-ribofuranosylbenzimidazole. J. Cell Biol. 99:1984;672-679.
62. Haaf T., Hayman D. L., Schmid M. Quantitative determination of rDNA transcription units in vertebrate cells. Exp. Cell. Res. 193:1991;78-86.
63. Haaf T., Ward D. C. Inhibition of RNA polymerase II transcription causes chromatin decondensation, loss of nucleolar structure, and dispersion of chromosomal domains. Exp. Cell. Res. 224:1996;163-173.
64. Zeng C., Kim E., Warren S. L., Berget S. M. Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity. EMBO J. 16:1997;1401-1412.
65. Chambon P., Mandel J. L., Gissinger F., Kedinger C., Gross Bellard M., Hossenlopp P. Transcription of double-stranded viral and cellular DNAs by purified mammalian DNA-dependent RNA polymerases. Basic. Life Sci. 3:1974;257-268.
66. Zatsepina O. V., Voit R., Grummt I., Spring H., Semenov M. V., Trendelenberg M. F. The RNA polymerase I-specific transcription initiation factor UBF is associated with transcriptionally active and inactive ribosomal genes. Chromosoma. 102:1993;599-611.
67. Hozák P., Cook P. R., Schöfer C., Mosgöller W., Wachtler F. Site of transcription of ribosomal RNA and intra-nucleolar structure in HeLa cells. J. Cell Sci. 107:1994;639-648.
68. Cook P. R. The organization of replication and transcription. Science. 284:1999;1790-1795.
69. Pombo A., Jackson D. A., Hollinshead M., Wang Z., Roeder R. G., Cook P. R. Regional specialization in human nuclei: Visualization of discrete sites of transcription by RNA polymerase III. EMBO J. 18:1999;2241-2253.
70. Kimura H., Tao Y., Roeder R. G., Cook P. R. Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: Little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure. Mol. Cell. Biol. 19:1999;5383-5392.
71. Shaw P. J., Jordan E. G. The nucleolus. Annu. Rev. Cell Dev. Biol. 11:1995;93-121.
72. Jackson D. A., Hassan A. B., Errington R. J., Cook P. R. Visualization of focal sites of transcription within human nuclei. EMBO J. 12:1993;1059-1065.
73. Jackson D. A., Iborra F. J., Manders E. M. M., Cook P. R. Numbers and organization of RNA polymerases, nascent transcripts and transcription units in HeLa nuclei. Mol. Biol. Cell. 9:1998;1523-1536.