Human monoclonal autoantibody fragments from combinatorial antibody libraries directed to the U1snRNP associated U1C protein; epitope mapping, immunolocalization and V-gene usage

Molecular Immunology

Volumn 35, Issue 16, 1998, Pages 1045-1055

  Hoet, R M A ^a,b   Raats, J M H ^a   De Wildt, R ^a,c   Dumortier, H ^d   Muller, S ^d   Van Den Hoogen, F ^a   Van Venrooij, W J ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

^c MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^d INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

Autoantibodies; Coiled bodies; Combinatorial antibody libraries; Phage display; scFv; U1 snRNP; U1C protein; V genes

Indexed keywords

AUTOANTIBODY; COMPLEMENTARY DNA; EPITOPE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ANTIBODY SPECIFICITY; ARTICLE; AUTOIMMUNITY; CROSS REACTION; GENE SEQUENCE; HELA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOPRECIPITATION; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHEUMATIC DISEASE; SYSTEMIC LUPUS ERYTHEMATOSUS;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; AUTOANTIBODIES; CROSS REACTIONS; EPITOPE MAPPING; GENES, IMMUNOGLOBULIN; HELA CELLS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOHISTOCHEMISTRY; LUPUS ERYTHEMATOSUS, SYSTEMIC; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RIBONUCLEOPROTEINS, SMALL NUCLEAR;

EID: 0032433306     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0161-5890(98)00093-5     Document Type: Article

References (35)

1. Andrade, L.E., Chan, E.K., Raska, I., Peebles, C.L., Roos, G., Tan, E.M., 1991. Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin. J. Exp. Med. 173, 1407-1419.
2. Barakat, S., Briand, J.P., Abuaf, N., Van Regenmortel, M.H.V., Muller, S., 1991. Mapping of epitopes on U1 snRNP polypeptide A with synthetic peptides and autoimmune sera. Clin. Exp. Immunol. 86, 71-78.
3. Brahms, H., Raker, V.A., van Venrooij, W.J., Lührmann, R., 1997. A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states. Arthritis Rheum. 40, 672-682.
4. Carmo-Fonseca, M., Pepperkok, R., Carvalho, M.T., Lamond, A.I., 1992. Transcription-dependent colocalization of the U1, U2, U4, U6, and U5 snRNPs in coiled bodies. J. Cell Biol. 117, 1-14.
5. De Wildt, R.M., Finnern, R., Ouwehand, W.H., Griffiths, A.D., van Venrooij, W.J., Hoet, R.M., 1996. Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and patient-derived combinatorial V gene library. Eur. J. Immunol. 26, 629-639.
6. Dumortier, H., Klein Gunnewick, J., Roussel, J-P., van Aarssen, Y., Briand, J-P., van Venrooij, W.J., Muller, S., 1998. At least three linear regions but not the zinc finger domain of U1C protein are exposed at the surface of the protein in solution and on the human spliceosomal U1 snRNP particle. Nucleic Acids Res., in press.
7. Finnern, R., Pedrollo, E., Fisch, I., Wieslander, J., Marks, J.D., Lockwood, C.M., Ouwehand, W.H., 1997. Human autoimmune antiproteinase 3 scFv from a phage display library. Clin. Exp. Immunol. 107, 269-281.
8. Griffiths, A.D., Williams, S.C., Hartley, O., Tomlinson, I.M., Waterhouse, P., Crosby, W.L., Kontermann, R.E., Jones, P.T., Low, N.M., Allison, T.J. et al., 1994. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
9. Habets, W.J., Hoet, M., Bringmann, P., Lührmann, R., van Venrooij, W.J. 1985. Autoantibodies to ribonucleoprotein particles containing U2 small nuclear RNA. EMBO J. 4, 1545-1550.
10. Habets, W.J., Sillekens, P.T., Hoet, M.H., McAllister, G., Lerner, M.R., van Venrooij, W.J., 1989. Small nuclear RNA-associated proteins are immunologically related as revealed by mapping of autoimmune reactive B-cell epitopes. Proc. Natl. Acad. Sci. U.S.A. 86, 4674-4678.
11. Halimi, H., Dumortier, H., Briand, J.P., Muller, S., 1996. Comparison of two different methods using overlapping synthetic peptides for localizing linear B cell epitopes in the U1 snRNP-C autoantigen. J. Immunol. Methods 199, 77-85.
12. Heinrichs, V., Bach M., Winkelmann, G., Luhrmann, R., 1990. U1-specific protein C needed for efficient complex formation of U1snRNP with a 5′ splice site. Science 247, 69-72.
13. Hoet, R.M., De Weerd, P., Gunnewiek, J.K., Koornneef, I., Van Venrooij, W.J., 1992. Epitope regions on U1 small nuclear RNA recognized by anti-U1RNA-specific autoantibodies. J. Clin. Invest. 90, 1753-1762.
14. James, J.A., Harley, J.B., 1995. Peptide autoantigenicity of the small nuclear ribonucleoprotein C. Clin. Exp. Rheumatol. 13, 299-305.
15. James, J.A., Gross, T., Scofield, R.H., Harley, J.B., 1995. Immunoglobulin epitope spreading and autoimmune disease after peptide immunization: Sm B/B′-derived PPPGMRPP and PPPGIRGP induce spliceosome autoimmunity. J. Exp. Med. 181, 453-461.
16. James, J.A., Harley, J.B., 1996. Human lupus anti-spliceosome A protein autoantibodies bind contiguous surface structures and segregate into two sequential epitope binding patterns. J. Immunol. 156, 4018-4026.
17. James, J.A., Scofield, R.H., Harley, J.B., 1997. Lupus humoral autoimmunity after short peptide immunization. In: B lymphocytes and autoimmunity. Annals New York Academy Sciences 815, 124-127.
18. Klein Gunnewiek, J.M., van Aarssen, Y., Wassenaar, R., Legrain, P., van Venrooij, W.J., Nelissen, R.L., 1995. Homodimerization of the human U1 snRNP-specific protein C. Nucleic Acids Res. 23, 4864-4871.
19. Klein Gunnewiek, J.M., van de Putte, L.B., van Venrooij, W.J., 1997. The U1 snRNP complex: an autoantigen in connective tissue diseases. An update. Clin. Exp. Rheumatol. 15, 549-560.
20. Kreis, T.E., Lodish, H.F., 1986. Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface. Cell 46, 929-937.
21. Marks, J.D., Hoogenboom, U.R., Bonnert, T.P., McCafferty, J., Griffiths, A.D., Winter, G., 1991. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
22. Misaki, Y., Yamamoto, K., Yanagi, K., Miura, H., Ichijo, H., Kato, T., Mato, T., Welling-Wester, S., Nishioka, K., Ito, K., 1993. B cell epitope on the U1 snRNP-C autoantigen contains a sequence similar to that of the herpes simplex virus protein. Eur. J. Immunol. 23, 1064-1071.
23. Nelissen, R.L., Heinrichs, V., Habets, W.J., Simons, F., Lührmann, R., van Venrooij, W.J., 1991. Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP. Nucleic Acids Res. 19, 449-454.
24. Nelissen, R.L., Will, C.L., van Venrooij, W.J., Lührmann, R., 1994. The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins. EMBO J. 13, 4113-4125.
25. Nissim, A., Hoogenboom, H.R., Tomlinson, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a 'single pot' phage display library as immunochemical reagents. EMBO J. 13, 692-698.
26. Ohlin, M., Owman, H., Mach, M., Borrebaeck, C.A., 1996. Light chain shuffling of a high affinity antibody results in a drift in epitope recognition. Mol. Immunol. 33, 47-56.
27. Olee, T., Yang, P., Siminovitch, K.A., Olsen, N.J., Hillson, J., Wu J., Kozin, F., Carson, D.A., Chen, P.P., 1991. Molecular basis of an autoantibody-associated restriction fragment length polymorphism that confers susceptibility to autoimmune diseases. J. Clin. Invest. 88, 193-203.
28. Rossi, F., Forne, T., Antoine, E., Tazi, J., Brunel, C., Cathala, G., 1996. Involvement of U1 small nuclear ribonucleoproteins (snRNP) in 5′ splice site-U1 snRNP interaction. J. Biol. Chem. 271, 23985-23991.
29. Sambrook, J., Fritsch, E., Maniatis, T., 1989. Molecular cloning. Cold Spring Harbor Lab Press ISBN 0-87969-309-6.
30. Satoh, M., Langdon, J.J., Hamilton, K.J., Richards, H.B., Panka, D., Eisenberg, R.A., Reeves, W.H., 1996. Distinctive immune response patterns of human and murine autoimmune sera to U1 small nuclear ribonucleoprotein C protein. J. Clin. Invest. 97, 2619-2626.
31. Satoh, M., Richards, H.B., Hamilton, K.J., Reeves, W.H., 1997. Human anti-nuclear ribonucleoprotein antigen autoimmune sera contain a novel subset of autoantibodies that stabilizes the molecular interaction of U1RNP-C protein with the Sm core proteins. J. Immunol. 158, 5017-5025.
32. Tang, J., Abovich, N., Fleming, M.L., Seraphin, B., Rosbash, M., 1997. Identification and characterization of a yeast homolog of U1snRNP-specific protein C. EMBO J. 16, 4082-4091.
33. Tsikaris, V., Vlachoyiannopoulos, P.G., Panou-Pomonis, E., Marraud, M., Sakarellos, C., Moutsopoulos, H.M., Sakarellos-Daitsiotis, M., 1996. Immunoreactivity and conformation of the P-P-G-M-R-P-P repetitive epitope of the Sm autoantigen. Int. J. Pept. Protein Res. 48, 319-327.
34. Will, C.L., Rumpler, S., Klein Gunnewiek, J., van Venrooij, W.J., Lührmann, R., 1996. In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes. Nucleic Acids Res. 24, 4614-4623.
35. Williams, D.G., Sharpe, N.G., Wallace, G., Latchman, D.S., 1990. A repeated proline-rich sequence in Sm B, B' and N is a dominant epitope recognized by human and murine autoantibodies. J. Autoimmunity 3, 715-725.