Effect of glutamine limitation on the death of attached Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 64, Issue 1, 1999, Pages 46-53

  Sanfeliu, Anna ^a   Stephanopoulos, Gregory ^a  

^a USA   (United States)

Author keywords

Cell cycle; Cell death; CHO cells; Glutamine synthetase; Insulin; Nutrient deprivation

Indexed keywords

BIOLOGY; BIOSYNTHESIS; BIOTECHNOLOGY; CELL CULTURE; CELLS; GROWTH KINETICS; INSULIN; ORGANIC ACIDS;

CELL CYCLE; CELL DEATH; CELL GROWTH RATE; GLUTAMIC ACID; GLUTAMINE; GLUTAMINE SYNTHETHASE; NUTRIENT DEPRIVATION; TRANSFECTION;

SUGARS;

GLUTAMATE AMMONIA LIGASE; GLUTAMINE; PROTEIN BCL 2;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL DEATH; CELL GROWTH; CELL PROLIFERATION; CHINESE HAMSTER; ENZYME ACTIVITY; GENE INDUCTION; GENETIC TRANSFECTION; NONHUMAN; ONCOGENE C MYC; OVARY CELL; PROTO ONCOGENE;

ANIMALS; CELL CYCLE; CELL DEATH; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GENES, BCL-2; GLUTAMATE-AMMONIA LIGASE; GLUTAMINE; HUMANS; TRANSFECTION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0033526894     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990705)64:1<46::AID-BIT5>3.0.CO;2-9     Document Type: Article

References (24)

1. Ardawi MSM, Newsholme EA. 1984. Glutamine metabolism in lymphoid tissues. In: Haussinger D, Sies H, editors. Glutamine metabolism in mammalian tissues. Berlin: Springer-Verlag. p 235-245.
2. Bhandari B, Wilson RH, Miller RE. 1987. Insulin and dexamethasone stimulate transcription of an amplified glutamine synthetase gene in Chinese hamster ovary cells. Mol Endocrinol 1:403-407.
3. Chung JD, Sinskey AJ, Stephanopoulos G. 1998. Growth factor and bcl-2 mediated survival during abortive proliferation of a hybridoma cell line. Biotechnol Bioeng 57:164-171.
4. Cotter TG, Al-Rubeai M. 1995. Cell death (apoptosis) in cell culture systems. Trends Biotechnol 13:150-155.
5. Evan GI, Wyllie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, Waters CM, Penn LZ, Hanckock DC. 1992. Induction of apoptosis in fibroblasts by c-Myc protein. Cell 69: 119-128.
6. Franek F, Dolniková J. 1991. Hybridoma growth and monoclonal antibody production in iron-rich protein free medium: Effect of nutrient concentration. Cytotechnology 7:33-38.
7. Franek F, Dolnikova J. 1991. Nucleosomes occuring in protein-free hybridoma cell culture. Evidence for programmed cell death. FEBS Lett 284:285-287.
8. Griffiths JB. 1973. The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism. J Cell Sci 12:617-629.
9. Guidotti GG, Borghetti AF, Cazzola GC. 1978. The regulation of amino acid transport in animal cells. Biochim Biophys Acta 515:329-366.
10. Hann SR, King MW, Bentley DL, Anderson CW, Eisenman RN. 1988. A non-AUG translational initiation in c-myc exon1 generates an N-terminal distinct protein whose synthesis is disrupted in Burkitt's lymphomas. Cell 52:185-195.
11. Kulka RG, Cohen H. 1973. Regulation of glutamine synthetase of hepatoma tissue culture cells by glutamine and dexamethasone. J Biol Chem 248: 6738-6743.
12. McDermott RH, Butler M. 1993. Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. J Cell Sci 104:51-58.
13. Mercille S, Massie B. 1994. Induction of apoptosis in nutrient deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44: 1140-1154.
14. Miller RE, Hackenberg R, Gershman H. 1978. Regulation of glutamine synthetase in cultured 3T3-L1 cells by insulin, hydrocortisone, and dibutyril cyclic AMP. Proc Natl Acad Sci USA 75:1418-1422.
15. Milman G, Portnoff LS, Tiemeier DC. 1974. Immunochemical evidence for glutamine-mediated degradation of glutamine synthetase in cultured Chinese hamster cells. J Biol Chem 250:1393-1399.
16. Murray KM, Ang C, Gull K, Hickman JA, Dickson AJ. 1996. NSO myeloma cell death: Influence of bcl-2 over-expression. Biotechnol Bioeng 51:298-304.
17. Nunez G, London L, Hockenbery D, Alexander M. McKearn JP, Korsmeyer SJ. 1990. Deregulated bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines. J Immunol 144:3602-3610.
18. Ramsay G, Evan GI, Bishop JM. 1984. The protein encoded by the human proto-oncogene c-myc. Proc Natl Acad Sci USA 81:7742-7746.
19. Reitzer LJ, Wice BM, Kennell D. 1979. Evidence that glutamine not sugar is the major energy source for cultured HeLa cells. J Biol Chem 254:197-203.
20. Sanfeliu A, Cairó J-J, Casas C, Solà C. Gòdia F. 1996. Analysis of nutritional factors and physical conditions affecting growth and monoclonal antibody production of the hybridoma KB-26.5 cell line. Biotechnol Prog 12:209-216.
21. Sanfeliu A, Chung J, Stephanopoulos G. 1998. Effect of insulin stimulation on the proliferation and cell death of Chinese hamster ovary cells. Submitted.
22. Singh RP, Al-Rubeai M, Gregory CD, Emery AN. 1994. Cell death in bioreactors: A role for apoptosis. Biotechnol Bioeng 44:720-726.
23. Singh RP, Emery AN, Al-Rubeai M. 1996. Enhancement of survivability of mammalian cells by over-expression of the apoptosis supressorgene bcl2. Biotechnol Bioeng 52:166-175.
24. Zielke HR, Zielke CL, Ozand PT. 1984. Glutamine: A major energy source for cultured mammalian cells. Fed Proc Fed Am Soc Biol Med 43: 121-123.