Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase

Genetics

Volumn 162, Issue 1, 2002, Pages 217-227

  Beall, Eileen L ^a   Mahoney, Matthew B ^a   Rio, Donald C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSPOSASE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; DNA DAMAGE; DNA DEGRADATION; DNA TRANSPOSITION; DROSOPHILA; ENZYME ACTIVITY; GENETIC ANALYSIS; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM;

AMINO ACID SEQUENCE; ANIMALS; DROSOPHILA; GERM CELLS; MICROINJECTIONS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PHOSPHORYLATION; TRANSPOSASES;

ARACHNIDA; HEXAPODA; PROKARYOTA;

EID: 0036742490     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (67)

1. Adams, M. D., S. E. Celniker, R. A. Holt, C. A. Evans, J. D. Gocayne et al., 2000 The genome sequence of Drosophila melanogaster. Science 287: 2185-2195.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman et al., 1987 Current Protocols in Molecular Biology. John Wiley & Sons, New York
3. Banga, S. S., A. Velazqvez and J. B. Boyd, 1991 P transposition in Drosophila provides a new tool for analyzing postreplication repair and double-strand break repair. Mutat. Res. 255: 79-88.
4. Banin, S., L. Moyal, S. Shieh, Y. Taya, C. W. Anderson et al., 1998 Enhanced phosphorylation of p53 by ATM in response to DNA damage. Science 281: 1674-1677.
5. Beall, E. L., and D. C. Rio, 1996 Drosophila IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivo. Genes Dev. 10: 921-933.
6. Beall, E. L., and D. C. Rio, 1997 Drosophila P element transposase is a novel site-specific endonuclease. Genes Dev. 11: 2137-2151.
7. Beall, E. L., and D. C. Rio, 1998 Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro. EMBO J. 17: 2122-2136.
8. Blunt, T., N. J. Finnie, G. E. Taccioli, G. C. Smith, J. Demengeot et al., 1995 Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80: 813-823.
9. Canman, C. E., D. S. Lim, K. A. Cimprich, Y. Taya, K. Tamai et al., 1998 Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science 281: 1677-1679.
10. Chen, J., and F. W. Alt, 1993 Gene rearrangement and B-cell development. Curr. Opin. Immunol. 5: 194-200.
11. Craig, N. L., 1997 Target site selection in transposition. Annu. Rev. Biochem. 66: 437-474.
12. Critchlow, S. E., and S. P. Jackson, 1998 DNA end-joining: from yeast to man. Trends Biochem. Sci. 23: 394-398.
13. Curcio, M. J., and D. J. Garfinkel, 1999 New lines of host defense: inhibition of Tyl retrotransposition by Fus3p and NER/TFIIH. Trends Genet. 15: 43-45.
14. Durocher, D., and S. P. Jackson, 2001 DNA-PK, ATM and ATR as sensors of DNA damage: Variations on a theme? Curr. Opin. Cell. Biol. 13: 225-231.
15. Eggleston, W. B., D. M. Johnson-Schlitz and W. R. Engels, 1988 P-M hybrid dysgenesis does not mobilize other transposable element families in D. melanogaster. Nature 331: 368-370.
16. Elledge, S. J., 1996 Cell cycle checkpoints: preventing an identity crisis. Science 274: 1664-1672.
17. Engels, W. R., D. M. Johnson-Schlitz, W. B. Eggleston and J. Sved, 1990 High-frequency P element loss in Drosophila is homolog dependent. Cell 62: 515-525.
18. Feller, S. M., R. Ren, H. Hanafusa and D. Baltimore, 1994 SH2 and SH3 domains as molecular adhesives: the interactions of Crk and Abl. Trends Biochem. Sci. 19: 453-458.
19. Fugmann S. D., I. J. Villey, L. M. Ptaszek and D. G. Schatz, 2000 Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex. Mol. Cell 5: 97-107.
20. Gerasimova, T. I., L. J. Mizrokhi and G. P. Georgiev, 1984 Transposition bursts in genetically unstable Drosophila melanogaster. Nature 309: 714-716.
21. Giaccia, A. J., and M. B. Kastan, 1998 The complexity of p53 modulation: emerging patterns from divergent signals. Genes Dev. 12: 2973-2983.
22. Gloor, G. B., J. Moretti, J. Mouyal and K. J. Kefler, 2000 Distinct P-element excision products in somatic and germline cells of Drosophila melanogaster. Genetics 155: 1821-1830.
23. Hunter, T., 1995 When is a lipid kinase not a lipid kinase? When it is a protein kinase. Cell 83: 1-4.
24. Jeggo, P. A., A. M. Carr and A. R. Lehmann, 1998 Splitting the ATM: distinct repair and checkpoint defects in ataxia-telangiectasia. Trends Genet. 14: 312-316.
25. Jimenez, G. S., F. Bryntesson, M. I. Torres-Arzayus, A. Priestley, M. Beeche et al., 1999 DNA-dependent protein kinase is not required for the p53-dependent response to DNA damage. Nature 400: 81-83.
26. Jin, S., S. Inoue and D. T. Weaver, 1997 Functions of the DNA-dependent protein kinase. Cancer Surv. 29: 221-261.
27. Johnson, L. N., and M. O. O'Reilly, 1996 Control by phosphorylation. Curr. Opin. Struct. Biol. 6: 762-769.
28. Kaufman, P. D., and D. C. Rio, 1992 P element transposition in vitro proceeds by a cut-and-paste mechanism and uses GTP as a cofactor. Cell 69: 27-39.
29. Kennedy, A. K., and D. B. Haniford, 1996 Isolation and characterization of IS1O transposase separation of function mutants: identification of amino acid residues in transposase that are important for active site function and the stability of transposition intermediates. J. Mol. Biol. 256: 533-547.
30. Khanna, K. K., and S. P. Jackson, 2001 DNA double-strand breaks: signaling, repair and the cancer connection. Nat. Genet. 27: 247-254.
31. Kharbanda, S., R. Ren, P. Pandey, T. D. Shafman, S. M. Feller et al., 1995 Activation of the c-Abl tyrosine kinase in the stress response to DNA-damaging agents. Nature 376: 785-788.
32. Kharbanda, S., P. Pandey, S. Jin, S. Inoue, A. Bharti et al., 1997 Functional interaction between DNA-PK and c-Abl in response to DNA damage. Nature 386: 732-735.
33. Kim, D. R., Y. Dai, C. L. Mundy, W. Yang and M. A. Oettinger, 1999 Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase. Genes Dev. 13: 3070-3080.
34. Kim, S. T., D. S. Lim, C. E. Canman and M. B. Kastan, 1999 Substrate specificities and identification of putative substrates of ATM kinase family members. J. Biol. Chem. 274: 37538-37543.
35. Ko, L. J., and C. Prives, 1996 p53: puzzle and paradigm. Genes Dev. 10: 1054-1072.
36. Kusano, K., D. M. Johnson-Schlitz and W. R. Engels, 2001 Sterility of Drosophila with mutations in the Bloom syndrome gene - complementation by Ku70. Science 291: 2600-2602.
37. Lampe, D. J., B. J. Akkrley, E. J. Rubin, J. J. Mekalanos and H. M. Robertson, 1999 Hyperactive transposase mutants of the Himarl mariner transposon. Proc. Natl. Acad. Sci. USA 96: 11428-11433.
38. Landree, M. A., J. A. Wibbenmeyer and D. B. Roth, 1999 Mutational analysis of RPAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination. Genes Dev. 13: 3059-3069.
39. Lee, C. C., Y. M. Mul and D. C. Rio, 1996 The Drosophila P-element KP repressor protein dimerizes and interacts with multiple sites on P-element DNA. Mol. Cell. Biol. 16: 5616-5622.
40. Lee, C. C., E. L. Beall and D. C. Rio, 1998 DNA binding by the KP repressor protein inhibits P element transposase activity in vitro. EMBO J. 17: 4166-4174.
41. Lee, J., and S. Desiderio, 1999 Cyclin A/CDK2 regulates V(D)J recombination by coordinating RAG-2 accumulation and DNA repair. Immunity 11: 771-781.
42. Lees-Miller, S. P., K. Sakaguchi, S. J. Ullrich, E. Appella and C. W. Anderson, 1992 Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53. Mol. Cell. Biol. 12: 5041-5049.
43. Lieber, M. R., U. Grawunder, X. Wu and M. Yaneva 1997 Tying loose ends: roles of Ku and DNA-dependent protein kinase in the repair of double-strand breaks. Curr. Opin. Genet. Dev. 7: 99-104.
44. McClintock, B., 1984 The significance of responses of the genome to challenge. Science 226: 792-801.
45. Mizuuchi, K., 1997 Polynucleotidyl transfer reactions in site-specific DNA recombination. Genes Cells 2: 1-12.
46. Mul, Y. M., and D. C. Rio, Reprogramming the purine nucleotide cofactor requirement of Drosophila P element transposase in Vivo. EMBO J. 16: 4441-4447.
47. Norbury, C., and P. Nurse, 1992 Animal cell cycles and their control. Annu. Rev. Biochem. 61: 441-470.
48. O'Brochta, D. A., and A. M. Handler, 1988 Mobility of P elements in drosophilids and nondrosophilids. Proc. Natl. Acad. Sci. USA 85: 6052-6056.
49. Pandita, T. K., H. B. Lieberman, D. S. Lim, S. Dhar, W. Zheng et al., 2000 Ionizing radiation activates the ATM kinase throughout the cell cycle. Oncogene 19: 1386-1391.
50. Qiu, J. X., S. B. Kale, H. Yarnell Schultz and D. B. Roth, 2001 Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination. Mol. Cell 7: 77-87.
51. Reznikoff, W. S., A. Bhasin, D. R. Davies, I. Y. Goryshin, L. A. Mahnke et al., 1999 Tn5: a molecular window on transposition. Biochem. Biophys. Res. Commun. 266: 729-734.
52. Rio, D. C., F. A. Laski and G. M. Rubin, 1986 Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell 44: 21-32.
53. Sakai, J., and N. Kleckner, 1996 Two classes of Tn10 transposase mutants that suppress mutations in the Tn10 terminal inverted repeat. Genetics 144: 861-870.
54. Sekelsky, J. J., K. C. Burtis and R. S. Hawley, 1998 Damage control: the pleiotropy of DNA repair genes in Drosophila melanogaster. Genetics 148: 1587-1598
55. Sekelsky, J. J., M. H. Brodsky and K. C. Burtis, 2000 DNA repair in Drosophila: insights from the Drosophila genome sequence. J. Cell Biol. 150: F31-36.
56. Shieh, S. Y., M. Ikeda, Y. Taya and C. Prives, 1997 DNA damageinduced phosphorylation of p53 alleviates inhibition by MDM2. Cell 91: 325-334.
57. Smith, G. C., and S. P. JACKSON, 1999 The DNA-dependent protein kinase. Genes Dev. 13: 916-934.
58. Songyang, Z., G. Gish, G. Mbamalu, T. Pawson and L. C. Cantley, 1995 A single point mutation switches the specificity of group III Src homology, (SH) 2 domains to that of group I SH2 domains. J. Biol. Chem. 270: 26029-26032.
59. Spradling A. C., 1986 P element-mediated transformation, pp. 175-197 in Drosophila: A Practical Approach, edited by D. Roberts. IRL Press, Oxford.
60. Staveley, B. E., T. R. Heslip, R. B. Hodgetts and J. B. Bell, 1995 Protected P-element terminii suggest a role for the inverted-repeat-binding protein in transposase-induced gap repair in Drosophila melanogaster. Genetics 139: 1321-1329.
61. Stellwagen A. E., and N. L. Craig, 1997 Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145: 573-585.
62. Tibbetts, R. S., K. M. Brumbaugh, J. M. Williams, J. N. Sarkaria, W. A. Cliby et al., 1999 A role for ATR in the DNA damage-induced phosphorylation of p53. Genes Dev. 13: 152-157.
63. Wang, J. Y., 1993 Abl tyrosine kinase in signal transduction and cell-cycle regulation. Curr. Biol. 3: 35-43.
64. Woodruff, R. C., J. L. Blount and J. N. Thompson, Jr., 1987 Hybrid dysgenesis in D. melanogaster is not a general release mechanism for DNA transpositions. Science 237: 1206-1218.
65. Yarnell Schultz, H., M. A. Landree, J. X. Qiu, S. B. Kale and D. B. Roth, 2001 Joining-deficient RAG1 mutants block V(D)J recombination in vivo and hairpin opening in vitro. Mol. Cell 7: 65-75.
66. Zakian, V. A., 1995 ATM-related genes: What do they tell us about functions of the human gene? Cell 82: 685-687.
67. Zhou, B. B., and S. J. Elledge, 2000 The DNA damage response: putting checkpoints in perspective. Nature 408: 433-439.