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Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
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of outstanding interest. The authors of this study describe the finding that RAG1 and RAG2, purified from insect cells that were baculovirus transfected, are sufficient to carry out the cleavage steps of V(D)J recombination.
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McBlane JF, Gent DCV, Ramsden DA, Romeo C, Cuomo CA, Gellert M, Oettinger MA. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. of outstanding interest Cell. 83:1995;387-395 The authors of this study describe the finding that RAG1 and RAG2, purified from insect cells that were baculovirus transfected, are sufficient to carry out the cleavage steps of V(D)J recombination.
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Van Gent, D.C.1
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15
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0025778519
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A link between double-strand break-related repair and V(D)J recombination: The scid mutation
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Hendrickson EA, Qin S, Bump EA, Schatz DG, Oettinger M, Weaver DT. A link between double-strand break-related repair and V(D)J recombination: the scid mutation. Proc Natl Acad Sci. 88:1991;4061-4065.
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Hendrickson, E.A.1
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Schatz, D.G.4
Oettinger, M.5
Weaver, D.T.6
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16
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0026071588
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Scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair
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Biedermann KA, Sun J, Giaccia AJ, Tosto TM, Brown JM. scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. Proc Natl Acad Sci. 88:1991;1394-1397.
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Biedermann, K.A.1
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Tosto, T.M.4
Brown, J.M.5
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17
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0029111784
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Gene for the catalytic subunit of the human DNA-a ctivated protein kinase maps to the site of the XRCC 7 gene on chromosome 8
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of special interest. The authors of this report describe the identification of the human chromosome to which the DNA-PK gene maps. This pericentric region of Chromosone 8 is the region which other studies have shown complement the murine scid phenotype. This provided important evidence that DNA-PK is the murine SCID factor.
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Sipley JC, Menninger JC, Hartley KO, Ward DC, Jackson SP, Anderson CW. Gene for the catalytic subunit of the human DNA-a ctivated protein kinase maps to the site of the XRCC 7 gene on chromosome 8. of special interest Proc Natl Acad Sci USA. 92:1995;7515-7519 The authors of this report describe the identification of the human chromosome to which the DNA-PK gene maps. This pericentric region of Chromosone 8 is the region which other studies have shown complement the murine scid phenotype. This provided important evidence that DNA-PK is the murine SCID factor.
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Proc Natl Acad Sci USA
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Sipley, J.C.1
Menninger, J.C.2
Hartley, K.O.3
Ward, D.C.4
Jackson, S.P.5
Anderson, C.W.6
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18
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0028784516
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Gene for the catalytic subunit of mouse DNA-PK maps to the scid locus
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of special interest. In this study, the human DNA-PK cDNA is used as a probe to determine the location of the murine counterpart. The authors map the gene to the chromosomal location where the murine scid phenotype had previously been mapped.
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Miller RD, Hogg J, Ozaki J, Gell J, Jackson SP, Riblet R. Gene for the catalytic subunit of mouse DNA-PK maps to the scid locus. of special interest Proc Natl Acad Sci USA. 92:1995;10792-10795 In this study, the human DNA-PK cDNA is used as a probe to determine the location of the murine counterpart. The authors map the gene to the chromosomal location where the murine scid phenotype had previously been mapped.
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Proc Natl Acad Sci USA
, vol.92
, pp. 10792-10795
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Miller, R.D.1
Hogg, J.2
Ozaki, J.3
Gell, J.4
Jackson, S.P.5
Riblet, R.6
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19
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0028960511
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Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation
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Blunt T, Finnie NJ, Taccioli GE, Smith GCM, Demengeot J, Gottlieb TM, Mizuta R, Varghese AJ, Alt FW, Jeggo PA, Jackson SP. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell. 80:1995;813-823.
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Blunt, T.1
Finnie, N.J.2
Taccioli, G.E.3
Smith, G.C.M.4
Demengeot, J.5
Gottlieb, T.M.6
Mizuta, R.7
Varghese, A.J.8
Alt, F.W.9
Jeggo, P.A.10
Jackson, S.P.11
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20
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0028902932
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Loss of the catalytic subunit of DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells
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Peterson SR, Kurimasa A, Oshimura M, Dynan WS, Bradbury EM, Chen DJ. Loss of the catalytic subunit of DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells. Proc Natl Acad Sci USA. 92:1995;3171-3174.
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Peterson, S.R.1
Kurimasa, A.2
Oshimura, M.3
Dynan, W.S.4
Bradbury, E.M.5
Chen, D.J.6
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21
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0029788520
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Identification of a nonsense mutation in the carboxy-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse
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Blunt T, Gell D, Fox M, Taccioli G, Lehman A, Jackson S, Jeggo PA. Identification of a nonsense mutation in the carboxy-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse. Proc Natl Acad Sci USA. 93:1996;10285-10290.
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Blunt, T.1
Gell, D.2
Fox, M.3
Taccioli, G.4
Lehman, A.5
Jackson, S.6
Jeggo, P.A.7
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22
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0029791450
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Biochemical and genetic defects in DNA-dependent protein kinase in murine scid lymphocytes
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Danska JS, Holland DP, Mariathasan S, Williams KM, Guidos CJ. Biochemical and genetic defects in DNA-dependent protein kinase in murine scid lymphocytes. Mol Cell Biol. 16:1996;5507-5517.
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Danska, J.S.1
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Williams, K.M.4
Guidos, C.J.5
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23
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0028788816
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Human DNA-PK is homologous to PI kinases
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Poltoratsky VP, Shi X, York JD, Lieber MR, Carter TH. Human DNA-PK is homologous to PI kinases. J Immunol. 155:1995;4529-4533.
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Poltoratsky, V.P.1
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Carter, T.H.5
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24
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0029156599
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DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product
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of outstanding interest. This paper reports the sequence of the human DNA-PK gene, allowing it to be identified as a member of the PI3 kinase family. There is a 31 amino acid segment missing from the middle of the kinase domain in this version of the sequence. The authors of [23] report the corrected sequence of the carboxy-terminal 100 kDa, which includes the kinase domain.
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Hartley KO, Gell D, Smith GCM, Zhang H, Divecha N, Lees-Miller SP, Anderson CW, Jackson SP. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. of outstanding interest Cell. 82:1995;849-856 This paper reports the sequence of the human DNA-PK gene, allowing it to be identified as a member of the PI3 kinase family. There is a 31 amino acid segment missing from the middle of the kinase domain in this version of the sequence. The authors of [23] report the corrected sequence of the carboxy-terminal 100 kDa, which includes the kinase domain.
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Cell
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Hartley, K.O.1
Gell, D.2
Smith, G.C.M.3
Zhang, H.4
Divecha, N.5
Lees-Miller, S.P.6
Anderson, C.W.7
Jackson, S.P.8
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25
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0028856331
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Hunter T. When is a lipid kinase not a lipid kinase? When it is a protein kinase. Cell. 83:1995;1-4.
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Hunter, T.1
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0029127643
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ATM-related genes: What do they tell us about functions of the human gene?
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Zakian VA. ATM-related genes: what do they tell us about functions of the human gene? Cell. 82:1995;685-687.
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Zakian, V.A.1
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0025201179
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28
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0025224761
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Human cells contain a DNA-activated protein kinase that phosphorylates SV40 T antigen, mouse p53, and the human Ku autoantigen
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Lees-Miller SP, Chen Y-R, Anderson CW. Human cells contain a DNA-activated protein kinase that phosphorylates SV40 T antigen, mouse p53, and the human Ku autoantigen. Mol Cell Biol. 10:1990;6472-6481.
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Lees-Miller, S.P.1
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Anderson, C.W.3
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29
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0029143646
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Scid cells are deficient in Ku and replication protein A phosphorylation by DNA-PK
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of special interest. A description of the only in vivo phosphorylation defects known at present in DNA-PK mutants, namely RP-A, and Ku. It will be important to determine whether these or other proteins are the key phosphorylation targets.
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Boubnov N, Weaver DT. scid cells are deficient in Ku and replication protein A phosphorylation by DNA-PK. of special interest Mol Cell Biol. 15:1995;5700-5706 A description of the only in vivo phosphorylation defects known at present in DNA-PK mutants, namely RP-A, and Ku. It will be important to determine whether these or other proteins are the key phosphorylation targets.
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Boubnov, N.1
Weaver, D.T.2
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30
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0030009738
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The DNA-dependent protein kinase is inactivated by autophosphorylation of the catalytic subunit
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of outstanding interest. The authors show that DNA-PK may undergo autophosphorylation itself, resulting in the loss of DNA dependent protein kinase activity.
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Chan DW, Lees-Miller SP. The DNA-dependent protein kinase is inactivated by autophosphorylation of the catalytic subunit. of outstanding interest J Biol Chem. 271:1996;8936-8941 The authors show that DNA-PK may undergo autophosphorylation itself, resulting in the loss of DNA dependent protein kinase activity.
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Chan, D.W.1
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Mimori T, Hardin JA. Mechanism of interaction between Ku protein and DNA. J Biol Chem. 261:1986;10375-10379.
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Hardin, J.A.2
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33
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0029954821
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Mutations in two Ku homologs define a DNA end-joining repair pathway in S. cerevisiae
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of outstanding interest. This work extends the study of DNA and joining to yeast and shows that knockout of yeast Ku reduces DNA end joining markedly.
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Milne GT, Jin S, Shannon KB, Weaver DT. Mutations in two Ku homologs define a DNA end-joining repair pathway in S. cerevisiae. of outstanding interest Mol Cell Biol. 16:1996;4189-4198 This work extends the study of DNA and joining to yeast and shows that knockout of yeast Ku reduces DNA end joining markedly.
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Milne, G.T.1
Jin, S.2
Shannon, K.B.3
Weaver, D.T.4
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0024356877
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HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complex
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Paillard S, Strauss F. Analysis of the mechanism of interaction of simian Ku with DNA. Nucleic Acids Res. 19:1991;5619-5624.
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The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen
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Gottlieb T, Jackson SP. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 72:1993;131-142.
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Gottlieb, T.1
Jackson, S.P.2
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0028897304
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DNA-dependent protein kinase activity is absent in xrs-6 cells: Implications for site-specific recombination and DNA double-strand break repair
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Finnie NJ, Gottlieb T, Blunt T, Jeggo P, Jackson SP. DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proc Natl Acad Sci USA. 92:1995;320-324.
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Finnie, N.J.1
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Blunt, T.3
Jeggo, P.4
Jackson, S.P.5
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38
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0027156278
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Impairment of V(D)J recombination in double-strand break repair mutants
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Taccioli GE, Rathbun G, Oltz E, Stamato T, Jeggo PA, Alt FW. Impairment of V(D)J recombination in double-strand break repair mutants. Science. 260:1993;207-210.
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Taccioli, G.E.1
Rathbun, G.2
Oltz, E.3
Stamato, T.4
Jeggo, P.A.5
Alt, F.W.6
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39
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0030576534
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Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates
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of outstanding interest. This study describes the knockout of Ku86, causing both the loss of signal and coding joint formation. Nevertheless, blunt signal ends and hairpinned coding ends are preserved.
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Zhu C, Bogue MA, Lim D-S, Hasty P, Roth DB. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. of outstanding interest Cell. 86:1996;379-389 This study describes the knockout of Ku86, causing both the loss of signal and coding joint formation. Nevertheless, blunt signal ends and hairpinned coding ends are preserved.
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Cell
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Zhu, C.1
Bogue, M.A.2
Lim, D.-S.3
Hasty, P.4
Roth, D.B.5
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0028112944
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Restoration of X-ray resistance and V(D)J recombination in mutant mammalian cells by Ku
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Smider V, Rathmell WK, Lieber M, Chu G. Restoration of X-ray resistance and V(D)J recombination in mutant mammalian cells by Ku. Science. 266:1994;288-291.
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Smider, V.1
Rathmell, W.K.2
Lieber, M.3
Chu, G.4
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41
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0028025020
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Ku80: Product of the XRCC5 gene and its role in DNA repair and V(D)J recombination
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Taccioli G, Gottlieb TM, Blunt T, Priestley A, Demengeot J, Mizuta R, Lehmann A, Alt FW, Jackson SP, Jeggo PA. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science. 265:1994;1442-1445.
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Taccioli, G.1
Gottlieb, T.M.2
Blunt, T.3
Priestley, A.4
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Mizuta, R.6
Lehmann, A.7
Alt, F.W.8
Jackson, S.P.9
Jeggo, P.A.10
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42
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0029778191
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Requirement for Ku80 in growth and immunoglobulin V(D)J recombination
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of outstanding interest. The knockout of Ku86 is described with similar findings to [39].
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Nussenzweig A, Chen C, Sores VDC, Sanchez M, Sokol K, Nussenzweig MC, Li GC. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination. of outstanding interest Nature. 382:1996;551-555 The knockout of Ku86 is described with similar findings to [39].
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Nature
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Nussenzweig, A.1
Chen, C.2
Sores, V.D.C.3
Sanchez, M.4
Sokol, K.5
Nussenzweig, M.C.6
Li, G.C.7
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43
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