The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability

EMBO Journal

Volumn 21, Issue 13, 2002, Pages 3526-3535

  Jimeno, S ^a   Rondon A G ^a   Luna, R ^a   Aguilera, A ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

Hpr1 Tho2; Mex67 Mtr2; RNA metabolism; Sub2 Yra1; THO complex

Indexed keywords

DNA; MESSENGER RNA; POLYPEPTIDE; PROTEIN HPR1; PROTEIN MFT1; PROTEIN THO2; PROTEIN THP2; RNA; UNCLASSIFIED DRUG;

ARTICLE; DNA RECOMBINATION; DNA SEQUENCE; GENE EXPRESSION; GENE MUTATION; GENETIC STABILITY; GENETIC TRANSCRIPTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RNA METABOLISM; RNA SPLICING; YEAST;

BIOLOGICAL TRANSPORT; DNA, FUNGAL; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; MACROMOLECULAR SUBSTANCES; MODELS, BIOLOGICAL; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; RECOMBINATION, GENETIC; RNA HELICASES; RNA, FUNGAL; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 0036645687     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf335     Document Type: Article

References (58)

1. The connection between transcription and genomic instability
2. HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene
3. A new type of fusion analysis applicable to many organisms: Protein fusions to the URA3 gene of yeast
4. SAP - A putative DNA-binding motif involved in chromosomal organization
5. Coupling RNA polymerase II transcription with pre-mRNA processing
6. Splice site selection, rate of splicing and alternative splicing on nascent transcripts
7. ALY, a context- dependent coactivator of LEF-1 and AML-1, is required for TCRα enhancer function
8. 32P labeling of RNA and DNA restriction fragments
9. A complex ccntaining RNA polymerase II, Paf1p, Cdc73p, Hpr1p and Ccr4p plays a role in protein kinase C signaling
10. The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability
11. A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae
12. Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae
13. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain
14. Coordination between transcription and pre-mRNA processing
15. Connections between the processing and nuclear export of mRNA: Evidence for an export license?
16. Inefficient processing impairs release of RNA from the site of transcription
17. Evidence for a role of Sky1p-mediated phosphorylation in 3′ splice site recognition involving both Prp8 and Prp17/Slu4
18. Transcription factor TFIID recruits factor CPSF for formation of 3′ end of mRNA
19. hnRNP proteins and the biogenesis of mRNA
20. Purification of two isoforms of hnRNP-U and characterization of their nucleic acid binding activity
21. High-copy-number expression of Sub2p, a member of the RNA helicase superfarmly, suppresses HPR1-mediated genomic instability
22. Capping, splicing and 3′ processing are independently stimulated by RNA polymerase II: Different functions for different segments of the CTD
23. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae
24. Yeast/E.coli shuttle vectors with multiple DNA transactions
25. RNA polymerase II and the integration of nuclear events
26. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
27. A comprehensive two-hybrid analysis to explore the yeast protein interactome
28. The DECD box putative ATPase Sub2p is an early mRNA export factor
29. Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast
30. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
31. Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for SUB2, an essential spliceosomal ATPase
32. Transcription of eukaryotic protein-coding genes
33. Messenger RNAs are recruited for nuclear export during transcription
34. Multiple roles for the yeast SUB2/yUAP56 gene in splicing
35. Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly
36. 5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II
37. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
38. mRNA polyadenylation and its coupling to other RNA processing reactions and to transcription
39. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
40. A novel yeast gene. THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination
41. YRA1, an essential Saccharomyces cerevisiae gene, encodes a novel nuclear protein with RNA annealing activity
42. Recombination between DNA repeats in yeast hpr1delta cells is linked to transcription elongation
43. Connecting transcription to messenger RNA processing
44. In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes
45. A generic protein purification method for protein complex characterization and proteome exploration
46. Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores
47. The Saccharomyces cerevisiae hyperrecombination mutant hpr1Delta is synthetically lethal with two conditional alleles of the acetyl coenzyme A carboxylase gene and causes a defect in nuclear export of polyadenylated RNA
48. + RNA and nuclear pores
49. Pre-mRNA processing and the CTD of RNA polymerase II: The tail that wags the dog?
50. Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
51. Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p
52. TREX is a conserved complex coupling transcription with RNA export
53. REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export
54. A multitude of suppressors of group II intron-splicing defects in yeast
55. Nuclear export of mRNA
56. The yeast hnRNP-like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p
57. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans
58. Transcription elongation: The 'Foggy' is lifting ellipsis