Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores

Molecular and Cellular Biology

Volumn 18, Issue 11, 1998, Pages 6826-6838

  Santos Rosa, Helena ^a   Moreno, Horacio ^a   Simos, George ^a   Segref, Alexandra ^a   Fahrenkrog, Birthe ^b   Panté, Nelly ^b,d   Hurt, Ed ^a,c  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF BASEL   (Switzerland)

^c Zentrum fur Molec Biologie   (Germany)

^d ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; MESSENGER RNA;

ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; GENE TRANSLOCATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FAMILY; RNA ANALYSIS; RNA SEQUENCE; RNA TRANSPORT;

ESCHERICHIA COLI;

EID: 0031772130     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.11.6826     Document Type: Article

References (54)

1. Amberg, D. C., A. L. Goldstein, and C. N. Cole. 1992. Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Genes Dev. 6:1173-1189.
2. Doye, V., and E. C. Hurt. 1997. From nucleoporins to nuclear pore complexes. Curr. Opin. Cell Biol. 9:401-411.
3. 2a. Fahrenkrog, B., E. Hurt, U. Aebi, and N. Panté. Submitted for publication.
4. Fischer, U., J. Huber, W. C. Boelens, I. W. Mattaj, and R. Lührmann. 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82:475-483.
5. Fornerod, M., M. Ohno, M. Yoshida, and I. W. Mattaj. 1997. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051-1060.
6. Fornerod, M., J. Vandeursen, S. Vanbaal, A. Reynolds, D. Davis, K. Gopal Murti, J. Fransen, and G. Grosveld. 1997. The human homologue of yeast Crm1 is in a dynamic subcomplex with Can/Nup214 and a novel nuclear pore component, Nup88. EMBO J. 16:807-816.
7. Fritz, C. C., and M. R. Green. 1996. HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs. Curr. Biol. 6:848-854.
8. Fukuda, M., S. Asano, T. Nakamura, M. Adachi, M. Yoshida, M. Yanagida, and E. Nishida. 1997. CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature 390:308-311.
9. Gerace, L. 1995. Nuclear export signals and the fast track to the cytoplasm. Cell 82:341-344.
10. Goldfarb, D. S. 1997. Nuclear transport-whose finger is on the switch? Science 276:1814-1816.
11. Görlich, D., M. Dabrowski, F. R. Bischoff, U. Kutay, P. Bork, E. Hartmann, S. Prehn, and E. Izaurralde. 1997. A novel class of RanGTP binding proteins. J. Cell Biol. 138:65-80.
12. Görlich, D., and I. W. Mattaj. 1996. Protein kinesis - nucleocytoplasmic transport. Science 271:1513-1518.
13. Gorsch, L. C., T. C. Dockendorff, and C. N. Cole. 1995. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129:939-955.
14. Grüter, P., C. Tabernero, C. von Kobbe, C. Schmitt, C. Saavedra, A. Bachi, M. Wilm, B. K. Felber, and E. Izaurralde. 1998. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol. Cell 1:649-659.
15. 13a. Hellmuth, K. Unpublished data.
16. Her, L. S., E. Lund, and J. E. Dahlberg. 1997. Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus. Science 276:1845-1848.
17. Hurt, E. C. 1988. A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J. 7:4323-4334.
18. 15a. Hurt, E. C. Unpublished data.
19. Iovine, M. K., and S. R. Wente. 1997. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J. Cell Biol. 137:797-811.
20. Izaurralde, E., A. Jarmolowski, C. Beisel, I. W. Mattaj, G. Dreyfuss, and U. Fischer. 1997. A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export. J. Cell Biol. 137:27-35.
21. Izaurralde, E., U. Kutay, C. von Kobbe, I. W. Mattaj, and D. Görlich. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16:6535-6547.
22. Kadowaki, T., M. Hitomi, S. Chen, and A. M. Tartakoff. 1994. Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant. Mol. Biol. Cell 5:1253-1263.
23. Kadowaki, T., Y. Zhao, and A. M. Tartakoff. 1992. A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm. Proc. Natl. Acad. Sci. USA 89:2312-2316.
24. Kahana, J., and P. Silver. 1996. Use of the A. victoria green fluorescent protein to study protein dynamics in vivo. Curr. Prot. Mol. Biol. 9:722-728.
25. Kitada, K., A. L. Johnson, L. H. Johnston, and A. Sugino. 1993. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol. Cell. Biol. 13:4445-4457.
26. Kutay, U., F. R. Bischoff, S. Kostka, R. Kraft, and D. Görlich. 1997. Export of importin a from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061-1071.
27. Lee, M. S., M. Henry, and P. A. Silver. 1996. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev. 10:1233-1246.
28. Lucocq, J. 1992. Quantitation of gold labeling and estimation of labeling efficiency with a stereological counting method. J. Histochem. Cytochem. 40:1929-1936.
29. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
30. Michael, W. M., M. Y. Choi, and G. Dreyfuss. 1995. A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway. Cell 83:415-422.
31. Muhlrad, D., R. Hunter, and R. Parker. 1992. A rapid method for localized mutagenesis of yeast genes. Yeast 8:79-82.
32. Murphy, R., and S. R. Wente. 1996. An RNA-export mediator with an essential nuclear export signal. Nature 383:357-360.
33. Nakielny, S., M. C. Siomi, H. Siomi, W. M. Michael, V. Pollard, and G. Dreyfuss. 1996. Transportin: nuclear transport receptor of a novel nuclear protein import pathway. Exp. Cell Res. 229:261-266.
34. Nigg, E. A. 1997. Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature 386:779-787.
35. Ossareh-Nazari, B., F. Bachelerie, and C. Dargemont. 1997. Evidence for a role of CRM1 in signal-mediated nuclear protein export. Science 278:141-144.
36. Panté, N., R. Bastos, I. McMorrow, B. Burke, and U. Aebi. 1994. Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J. Cell Biol. 126:603-617.
37. Pemberton, L. F., J. S. Rosenblum, and G. Blobel. 1997. A distinct and parallel pathway for nuclear import of an mRNA-binding protein. J. Cell Biol. 139:1645-1653.
38. Piñol-Roma, S., and G. Dreyfuss. 1992. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature 355:730-732.
39. Richards, S. A., K. L. Carey, and I. G. Macara. 1997. Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276:1842-1844.
40. Richards, S. A., K. M. Lounsbury, K. L. Carey, and I. G. Macara. 1996. A nuclear export signal is essential for the cytosolic localization of the ran binding protein, RanBP1. J. Cell Biol. 134:1157-1168.
41. Rout, M. P., G. Blobel, and J. D. Aitchison. 1997. A distinct nuclear import pathway used by ribosomal proteins. Cell 89:715-725.
42. 38a. Santos-Rosa, H. Unpublished data.
43. Schlaich, N. L., and E. C. Hurt. 1995. Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p. Eur. J. Cell Biol. 67:8-14.
44. 39a. Segref, A. Unpublished data.
45. + RNA and nuclear pores. EMBO J. 16:3256-3271.
46. Senger, B., G. Simos, F. R. Bischoff, A. V. Podtelejnikov, M. Mann, and E. C. Hurt. Mtr10p functions as a nuclear import receptor for the mRNA binding protein Np13p. Submitted for publication.
47. Shibasaki, F., E. R. Price, D. Milan, and F. McKeon. 1996. Role of kinases and the phosphatase calcineurin in the nuclear shuttling of transcription factor NF-AT4. Nature 382:370-373.
48. Simos, G., A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, and E. C. Hurt. 1996. The yeast protein Arc1p binds to tRNA and functions as a cofactor for methionyl-and glutamyl-tRNA synthetases. EMBO J. 15: 5437-5448.
49. Simos, G., H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E. C. Hurt. 1996. Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J. 15:2270-2284.
50. Siniossoglou, S., C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E. C. Hurt. 1996. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84:265-275.
51. Stade, K., C. S. Ford, C. Guthrie, and K. Weis. 1997. Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 90:1041-1050.
52. Stutz, F., M. Neville, and M. Rosbash. 1995. Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast. Cell 82:495-506.
53. Wimmer, C., V. Doye, P. Grandi, U. Nehrbass, and E. Hurt. 1992. A new subclass of nucleoporins that functionally interacts with nuclear pore protein NSP1. EMBO J. 11:5051-5061.
54. Yoon, D.-K., H. Lee, W. Seol, M. DeMaria, M. Rosenzweig, and J. U. Jung. 1997. Tap: a novel cellular protein that interacts with Tip of herpesvirus saimiri and induces lymphocyte aggregation. Immunity 6:571-582.