Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase

Genes and Development

Volumn 15, Issue 1, 2001, Pages 42-49

  Kistler, A L ^a   Guthrie, C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

DEAD box protein; Mud2; Pre mRNA splicing; Sub2

Indexed keywords

ADENOSINE TRIPHOSPHATASE; GENE PRODUCT; MUD2 PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN; SUB2 PROTEIN; U2AF65 PROTEIN; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; ENZYME ACTIVITY; GENETIC PROCEDURES; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPLICEOSOME; YEAST;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; ANIMALS; FUNGAL PROTEINS; GENE DELETION; HUMANS; MAMMALS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; RIBONUCLEOPROTEINS; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPLICEOSOMES;

MAMMALIA;

EID: 0035177309     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.851301     Document Type: Article

References (50)

1. Cross-intron bridging inter-actions in the yeast commitment complex are conserved in mammals
2. The yeast MUD2 protein: An interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition
3. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
4. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition
5. Helicases: A unifying structural theme?
6. Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD-box splicing factor
7. Spliceosome assembly in yeast
8. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
9. Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins
10. U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction
11. Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43
12. Guide to yeast genetics and molecular biology
13. Mutational analysis of the yeast DEAH-box splicing factor Prp16
14. Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase
15. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs
16. Interactions between small nuclear ribonucleoprorein particles in formation of spliceosomes
17. Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP
18. Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super-families of helicases
19. Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs
20. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
21. Requirements for U2 snRNP addition to yeast pre-mRNA
22. Multiple roles for the yeast SUB2/yUAP56 gene in splicing
23. Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast
24. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
25. Disruption of six Saccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutants
26. In vitro reconstitution of functional yeast U2 snRNPs
27. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
28. A rapid method for localized mutagenesis of yeast genes
29. Transcriptional pulse-chase analysis reveals a role for a novel snRNP-associated protein in the manufacture of spliceosomal snRNPs
30. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA
31. Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22
32. Mutational analysis of a DEAD box RNA helicase: The mammalian translation initiation factor eIF-4A
33. The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis
34. ATP can be dispensable for prespliceosome formation in yeast
35. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA
36. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly
37. Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly
38. A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing
39. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
40. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
41. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
42. Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection
43. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
44. The DEAH-box protein Prp22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
45. The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro
46. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation
47. Structure of the hepatitis C virus RNA helicase domain
48. Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
49. Human homologs of yeast Prp16 and Prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing