A review of fluorescence methods for assessing labile iron in cells and biological fluids

Analytical Biochemistry

Volumn 304, Issue 1, 2002, Pages 1-18

  Espósito, Breno P ^a   Epsztejn, Silvina ^a   Breuer, William ^a   Cabantchik, Z Ioav ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Chelators; Fluorescence; Iron; Oxidative stress; Transferrin; Transport

Indexed keywords

CHELATION; FLUORESCENCE; OXIDATIVE STRESS;

BIOLOGICAL FLUIDS; CHELATORS; DESIGN AND APPLICATION; FLUORESCENCE METHOD; FLUORESCENT PROBES; TOXICOLOGICAL PROPERTIES; TRANSFERRIN; TRANSPORT;

IRON;

FLUORESCENT DYE; IRON; IRON CHELATING AGENT; PROTEIN; TRANSFERRIN;

ANALYTIC METHOD; BIOCHEMISTRY; BIOSENSOR; BODY FLUID; CELL; DEVICE; FLUORESCENCE; IRON CHELATION; IRON TRANSPORT; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN BINDING; REVIEW; TOXICOLOGY;

EID: 0036570964     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5611     Document Type: Review

References (105)

1. Iron metabolism
2. An intracellular transit iron pool
3. Regulation of iron metabolism in eukaryotes
4. Nonspecific serum iron in thalassaemia: An abnormal serum iron fraction of potential toxicity
5. A non-transferrin-bound serum iron in idiopathic hemochromatosis
6. A direct method for quantification of non-transferrin-bound iron
7. Quantification of non-transferrin bound iron in the presence of unsaturated transferrin
8. The assessment of serum nontransferrin-bound iron in chelation therapy and iron supplementation
9. Bleomycin detectable iron in serum from laeukemiac patients before and after chemotherapy
10. Transient iron-overload with bleomycin-detectable iron present during cardiopulmonary bypass surgery
11. Bleomycin-detectable iron in the plasma of premature and full-term neonates
12. The importance of non-transferrin iron in disorders of iron metabolism
13. Transport of iron in the blood-brain-cerebrospinal fluid system
14. Evidence for low molecular weight, non-transferrin bound iron in rat brain and cerebrospinal fluid
15. Bleomycin-detectable iron in knee-joint synovial fluid from arthritic patients and its relationship to the extracellular antioxidant activities of caeruloplasmin, transferrin and lactoferrin
16. Iron-binding proteins and free iron in synovial fluids of rheumatoid arthritis patients
17. Iron, lactoferrin and iron regulatory protein activity in the synovium: Relative importance of iron loading in the inflammatory response
18. Quantitative studies of ferritinlike iron in erythrocytes of thalassemia, sickle-cell anemia, and hemoglobin Hammersmith with Mössbauer spectroscopy
19. Iron in Parkinson disease, blood diseases, malaria and ferritin
20. Advances in instrumental methods for the measurement and speciation of trace metals
21. Speciation of tissue and cellular iron with on-line detection by inductively coupled plasma-mass spectrometry
22. Intracellular iron in liver tissue and liver homegenate - Studies with electron paramagnetic resonance on the formation of paramagnetic complexes with desferal and nitric oxide
23. Colorimetric measurement of iron in plasma samples anticoagulated with EDTA
24. An improved simple colorimetric method for quantitation of non-transferrin-bound iron in serum
25. Fully automated measurement of total iron-binding capacity in serum
26. Determination of iron in calf serum using bathophenanthroline and thermal lens spectrometry
27. Comparative study of two methods for serum iron
28. Fluorescent probes of cell signaling
29. Interactions of transition-metal ions with photoexcited states of flavins. Fluorescence quenching studies
30. Quenching of excited singlet states by metal ions
31. Fluorimetric determination of iron using 5-(4-methoxyphenylazo)-8-(4-toluenesulfonamido)quinoline
32. Monitoring of iron(III) removal from biological sources using a novel fluorescent siderophore
33. Molecular recognition and signaling
34. Fluorescein and eosin as sensitizing chromophores in near-infrared luminescent ytterbium(III), neodymium(III) and erbium(III) chelates
35. A fluorescence-based one-step assay for serum non-transferrin bound iron (NTBI)
36. Fluorescent, siderophore-based chelators. Design and synthesis of a trispyrenyl trishydroxamate ligand, an intramolecular excimer-forming sensing molecule which responds to iron(III) and gallium(III) metal cations
37. Iron uptake in Ustilago maydis: Studies with fluorescent ferrichrome analogues
38. Iron uptake in Ustilago maydis: Tracking the iron path
39. Modular fluorescent-labeled siderophore analogues
40. Fluorescent siderophore-based chemosensors: Iron(III) quantitative determinations
41. Direct measurement of ferrous ion transport across membranes using a sensitive fluorometric assay
42. Continuous monitoring of transport by fluorescence on cells and vesicles
43. Chelation and mobilization of cellular iron by different classes of chelators
44. Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK
45. Determination of the chelatable iron pool of single intact cells by laser scanning microscopy
46. Iron acquired from transferrin by K562 cells is delivered into a cytoplasmic pool of chelatable iron(II)
47. Calcein as a fluorescent probe for ferric iron. Application to iron nutrition in plant cells
48. A fluorescence assay for assessing chelation of intracellular iron in a membrane model system and in mammalian cells
49. Desferrioxamine-chelatable iron (DCI), a component of serum non-transferrin bound iron (NTBI) used for assessing chelation therapy
50. Chelate mediated transfer of iron from transferrin to desferrioxamine
51. Iron removal from transferrin - An experimental study
52. Non-transferrin bound iron in plasma or serum from patients with idiopathic hemochromatosis
53. Measurement of iron and copper in biological systems: Bleomycin and copper-phenanthroline assays
54. Oxalate- and Ga(3+)-induced structural changes in human serum transferrin and its recombinant N-lobe. 1H NMR detection of preferential C-lobe Ga3+ binding
55. Role of ferritin in the control of the labile iron pool in murine erythroleukemia cells
56. Fluorescence analysis of the labile iron pool of mammalian cells
57. Dynamics of the cytosolic chelatable iron pool of K562 cells
58. The labile iron pool of hepatocytes in iron overload and chelator-induced iron deprivation
59. H-ferritin subunit overexpression in erythroid cells reduces the oxidative stress response and induces multidrug resistance properties
60. Repression of the heavy ferritin chain increases the labile iron pool of human K562 cells
61. Repression of ferritin expression increases the labile iron pool, oxidative stress and short-term growth of human erythroleukemia cells
62. Newly delivered transferrin iron and oxidative cell injury
63. Subcellular distribution of chelatable iron: A laser scanning microscopic study in isolated hepatocytes and liver endothelial cells
64. Transport of iron and other related metals into cells as revealed by a fluorescent probe
65. Multiphoton microscopy in life sciences
66. Iron quantification in cerebrospinal fluid
67. Patterns of mobilization of copper and iron following myocardial ischemia: Possible predictive criteria for tissue injury
68. Distribution of iron in reticulocytes after inhibition of heme synthesis with succinylacetone: Examination of the intermediates involved in iron metabolism
69. Recent evolution of luminescent photoinduced electron transfer sensors - A review
70. Combining luminescence, coordination and electron transfer for signalling purposes
71. Sensing of transition metals through fluorescence quenching or enhancement - A review
72. Unusually high fluorescence enhancement of some 1,8-naphtalimide derivatives induced by transition metal ions
73. Fluorescence signalling of transition metal ions by multi-component systems comprising 4-chloro-1,8-naphtalimide as fluorophore
74. Modulation of metal-fluorophore communication to develop structurally simple fluorescent sensors for transition metals
75. Transition metal ion induced fluorescent enhancement of 4-(N,N-dimethylethylenediamino)-7-nitrobenz-2-oxa-1,3-diazole
76. Transition metal (II)/(III), Eu(III), and Tb(III) ions induced molecular photonic OR gates using trianthryl cryptands of varying cavity dimension
77. 3+ ion
78. 2+ encapsulated in silica xerogel
79. Pirfenidone inhibits ADPH-dependent microsomal lipid peroxidation and scavenges hydroxyl radicals
80. Chemical determinants of antimalarial activity of reversed siderophores
81. Aminothiol multidentate chelators as antimalarials
82. Plasmodium falciparum and Plasmodium yoelli: Effect of the iron chelation prodrug dexrazoxane on in vitro cultures
83. Labile iron in parenteral iron formulations and its potential for generating plasma non-transferrin bound iron (NTBI) in dialysis patients
84. Chelation of iron within the erythrocytic Plasmodium falciparum parasite by iron chelators
85. Evidences that zidovudine (AZT) could not be directly responsible for iron overload in AZT-treated patients: An in vitro study
86. The labile iron pool in hepatocytes: Prooxidant-induced increase in free iron precedes oxidative cell injury
87. 2 formation
88. Induction of oxidative stress by humic acid through increasing intracellular iron: A possible mechanism leading to atherothrombotic vascular disorder in blackfoot disease
89. Role of ascorbic acid in transferrin-independent reduction and uptake of iron by U-937 cells
90. 2
91. Heme oxygenase activity causes transient hypersensitivity to oxidative ultraviolet A radiation that depends on release of iron from heme
92. Ultraviolet A radiation induces immediate release of iron in human primary skin fibroblasts: The role of ferritin
93. Overexpression of the ferritin iron-responsive element decreases the labile iron pool and abolishes the regulation of iron absorption by intestinal epithelial (Caco-2) cells
94. The cellular labile iron pool and intracellular ferritin in K562 cells
95. Cisplatin ototoxicity: Involvement of iron and enhanced formation of superoxide anion radicals
96. Nramp1: A link between intracellular iron transport and innate resistance to intracellular pathogens
97. G189 in RAW264.7 cell transfectants: Analysis of intracellular iron transport
98. Nramp2 (DCT1/DMT1) expressed at the plasma membrane transports iron and other divalent cations into a calcein-accessible cytoplasmic pool
99. Characterization of an integral protein of the brush border membrane mediating the transport of divalent metal ions
100. Copper-specific chelators as synergists to herbicides: 1. Amphiphilic dithiocarbamates, synthesis, transport through lipid bilayers, and inhibition of Cu/Zn superoxide dismutase activity
101. Spectrofluorimetric determination of iron(III) with 2-pyridinecarbaldehyde-5-nitro-pyridylhydrazone in the presence of hexadecyltrimethylmamonium bromide surfactant