The labile iron pool in hepatocytes: Prooxidant-induced increase in free iron precedes oxidative cell injury

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 274, Issue 6 37-6, 1998, Pages

  Stäubli, Anna ^a   Boelsterli, Urs A ^a,b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Calcein; Cultured murine hepatocytes; Iron chelators; Nitrofurantoin; Reductive stress

Indexed keywords

CALCEIN; IRON; NITROFURANTOIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DAMAGE; CELL ISOLATION; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IRON CHELATION; LIVER CELL; MALE; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; PATHOGENESIS; PRIORITY JOURNAL; STATISTICAL ANALYSIS;

EID: 0031744631     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1998.274.6.g1031     Document Type: Article

References (44)

1. Bacon, B. R., and A. S. Tavill. Role of the liver in normal iron metabolism. Semin. Liver Dis. 4: 181-192, 1984.
2. Baker, E., D. Richardson, S. Gross, and P. Ponka. Evaluation of the iron chelation potential of hydrazones of pyridoxal, salicylaldehyde and 2-hydroxy-1-naphthylaldehyde using the hepatocyte in culture. Hepatology 15: 492-501, 1992.
3. Beard, J. L., H. Dawson, and D. J. Pinero. Iron metabolism: a comprehensive review. Nutr. Rev. 54: 295-317, 1996.
4. Berry, M. N., and D. S. Friend. High-yield preparation of isolated rat liver parenchymal cells. A biochemical and fine structural study. J. Cell Biol. 43: 506-520, 1969.
5. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72: 248-254, 1976.
6. Breuer, W., S. Epsztejn, and Z. I. Cabantchik. Dynamics of the cytosolic chelatable iron pool of K562 cells. FEBS Lett. 382: 304-308, 1996.
7. Breuer, W., S. Epsztejn, and Z. I. Cabantchik. Iron acquired from transferrin by K562 cells is delivered into a cytoplasmic pool of chelatable iron(II). J. Biol. Chem. 270: 24209-24215, 1995.
8. Breuer, W., S. Epsztejn, P. Millgram, and Z. I. Cabantchik. Transport of iron and other related metals into cells as revealed by a fluorescent probe. Am. J. Physiol. 268 (Cell Physiol. 37): C1354-C1361, 1995.
9. Breuer, W., E. Greenberg, and Z. I. Cabantchik. Newly delivered transferrin iron and oxidative cell injury. FEBS Lett. 403: 213-219, 1997.
10. Cabantchik, Z. I., H. Glickstein, P. Milgram, and W. Breuer. A fluorescence assay for assessing chelation of intracellular iron in a membrane model system and in mammalial cells. Anal. Biochem. 233: 221-227, 1996.
11. Caraceni, P., D. H. Van Thiel, and A. B. Borle. Dual effect of deferoxamine on free radical formation and reoxygenation injury in isolated hepatocytes. Am. J. Physiol. 269 (Gastrointest. Liver Physiol. 32): G132-G137, 1995.
12. Epsztejn, S., O. Kakhlon, H. Glickstein, W. Breuer, and Z. I. Cabantchik. Fluorescence analysis of the labile iron pool of mammalian cells. Anal. Biochem. 248: 31-40, 1997.
13. Farber, J. L. Mechanisms of cell injury by activated oxygen species. Environ. Health Perspect. 102: 17-24, 1994.
14. Feller, N., H. J. Broxterman, D. C. R. Währer, and H. M. Pinedo. ATP-dependent efflux of calcein by the multidrug resistance protein (MRP): no inhibition by intracellular glutathione depletion. FEBS Lett. 368: 385-388, 1995.
15. Funk, F., J. P. Lenders, R. R. Crichton, and W. Schneider. Reductive mobilisation of ferritin iron. Eur. J. Biochem. 152: 167-172, 1985.
16. Galey, J. B. Potential use of iron chelators against oxidative damage. In: Antioxidants in Disease. Mechanisms and Therapy, edited by H. Sies. San Diego: Academic, 1997, p. 167-203.
17. Göldlin, C., and U. A. Boelsterli. Reactive oxygen species and non-peroxidative mechanisms of cocaine-induced cytotoxicity in rat hepatocyte cultures. Toxicology 69: 79-91, 1991.
18. Gunshin, H., B. Mackenzie, U. V. Berger, Y. Gunshin, M. F. Romero, W. F. Boron, S. Nussberger, J. L. Gollan, and M. A. Hediger. Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388: 482-488, 1997.
19. Gutteridge, J. M. C., D. A. Rowley, and B. Halliwell. Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts: detection of "free" iron in biological systems by using bleomycin-dependent degradation of DNA. Biochem. J. 199: 263-265, 1981.
20. Halliwell, B., and J. M. C. Gutteridge. Free Radicals in Biology and Medicine. New York: Oxford University, 1989.
21. Hollo, Z., L. Homolya, C. W. Davis, and B. Sarkadi. Calcein accumulation as a fluorometric functional assay of the multidrug transporter. Biochim. Biophys. Acta 1191: 384-388, 1994.
22. Hollo, Z., L. Homolya, T. Hegedus, and B. Sarkadi. Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells. FEBS Lett. 383: 99-104, 1996.
23. Jaeschke, H. Mechanisms of oxidant stress-induced acute tissue injury. Proc. Soc. Exp. Biol. Med. 209: 104-111, 1995.
24. Jaeschke, H. Reactive oxygen and ischemia/reperfusion injury of the liver. Chem. Biol. Interact. 79: 115-136, 1991.
25. Kehrer, J. P. Free radicals as mediators of tissue injury and disease. Crit. Rev. Toxicol. 23: 21-48, 1993.
26. Kozlov, A. V., A. Bini, D. Gallesi, F. Giovannini, A. Iannone, A. Masini, E. Meletti, and A. Tomasi. "Free" iron, as detected by electron paramagnetic resonance spectroscopy, increases unequally in different tissues during dietary iron overload in the rat. Biometals 9: 98-103, 1996.
27. Liminga, G., P. Nygren, and R. Larsson. Microfluorometric evaluation of calcein acetoxymethyl ester as a probe for P-glycoprotein-mediated resistance: effects of cyclosporin A and its nonimmunosuppressive analogue SDZ PSC 833. Exp. Cell Res. 212: 291-296, 1994.
28. Lin, M., R. A. Rippe, O. Niemela, G. Brittenham, and H. Tsukamoto. Role of iron in NF-κB activation and cytokine gene expression by rat hepatic macrophages. Am. J. Physiol. 272 (Gastrointest. Liver Physiol. 35): G1355-G1364, 1997.
29. Monteiro, H. P., G. F. Vile, and C. C. Winterbourn. Release of iron from ferritin by semiquinone, anthracycline, bipyridyl, and nitroaromatic radicals. Free Radic. Biol. Med. 6: 587-591, 1989.
30. Okada, S. Iron-induced tissue damage and cancer: the role of reactive oxygen species-free radicals. Pathol. Int. 46: 311-332, 1996.
31. Ollinger, K., and U. T. Brunk. Cellular injury induced by oxidative stress is mediated through lysosomal damage. Free Radic. Biol. Med. 19: 565-574, 1995.
32. Paterna, J. C., F. Boess, A. Stäubli, and U. A. Boelsterli. Antioxidant and cytoprotective properties of D-tagatose in cultured murine hepatocytes. Toxicol. Appl. Pharmacol. 148: 117-125, 1998.
33. Reif, D. W. Ferritin as a source of iron for oxidative damage. Free Radic. Biol. Med. 12: 417-427, 1992.
34. Rothman, R. J., A. Serroni, and J. L. Farber. Cellular pool of transient ferric iron, chelatable by deferoxamine and distinct from ferritin, that is involved in oxidative cell injury. Mol. Pharmacol. 42: 703-710, 1992.
35. Ryan, T. P., and S. D. Aust. The role of iron in oxygen-mediated toxicities. Crit. Rev. Toxicol. 22: 119-141, 1992.
36. Shertzer, H. G., G. L. Bannenberg, and P. Moldéus. Evaluation of iron binding and peroxide-mediated toxicity in rat hepatocytes. Biochem. Pharmacol. 44: 1367-1373, 1992.
37. Skibba, J. L., and E. A. Gwartney. Liver hyperthermia and oxidative stress: role of iron and aldehyde production. Int. J. Hyperthermia 13: 215-226, 1997.
38. Suzuki, Y. J., H. J. Forman, and A. Sevanian. Oxidants as stimulators of signal transduction. Free Radic. Biol. Med. 22: 269-285, 1997.
39. Thomas, C. E., and S. D. Aust. Free radicals and environmental toxins. Ann. Emerg. Med. 15: 1075-1083, 1986.
40. Thomas, C. E., and S. D. Aust. Reductive release of iron from ferritin by cation free radicals of paraquat and other bipyridyls. J. Biol. Chem. 261: 13064-13070, 1986.
41. Thomas, C. E., and S. D. Aust. Release of iron from ferritin by cardiotoxic anthracycline antiobiotics. Arch. Biochem. Biophys. 248: 684-689, 1986.
42. Wolf, A., C. Trendelenburg, C. Diez-Fernandez, P. Prieto, S. Houy, W. E. Trommer, and A. Cordier. Cyclosporine A-induced oxidative stress in rat hepatocytes. J. Pharmacol. Exp. Ther. 280: 1328-1334, 1997.
43. Zahrebelski, G., A. L. Nieminen, K. Alghoul, T. Qian, B. Herman, and J. J. Lemasters. Progression of subcellular changes during chemical hypoxia to cultured rat hepatocytes: a laser scanning confocal microscopic study. Hepatology 21: 1361-1372, 1995.
44. Zanninelli, G., H. Glickstein, W. Breuer, P. Milgram, P. Brissot, R. C. Hider, A. M. Konijn, J. Libman, A. Shanzer, and Z. I. Cabantchik. Chelation and mobilization of cellular iron by different classes of chelators. Mol. Pharmacol. 51: 842-852, 1997.