BJ fibroblasts display high antioxidant capacity and slow telomere shortening independent of hTERT transfection

Free Radical Biology and Medicine

Volumn 31, Issue 6, 2001, Pages 824-831

  Lorenz, Mario ^a   Saretzki, Gabriele ^b   Sitte, Nicolle ^c   Metzkow, Susanne ^a   Von Zglinicki, Thomas ^b,d  

^a HUMBOLDT UNIVERSITY   (Germany)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d NEWCASTLE GENERAL HOSPITAL   (United Kingdom)

Author keywords

Fibroblasts; Free radicals; Hyperoxia; Immortality; Senescence; Telomerase; Telomeres

Indexed keywords

CARBONYL DERIVATIVE; LIPOFUSCIN; PEROXIDE; TELOMERASE;

ANTIOXIDANT ACTIVITY; ARTICLE; CELL CLONE; CELL GROWTH; CELL IMMORTALIZATION; CELL STRAIN; CHROMOSOME SIZE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FETUS; FETUS LUNG; FIBROBLAST; GENETIC DAMAGE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYPEROXIA; LUNG FIBROBLAST; OXIDATIVE STRESS; PEROXIDATION; PREPUCE; PRIORITY JOURNAL; TELOMERE;

ANTIOXIDANTS; CATALYSIS; CELL DIVISION; CELL HYPOXIA; CELL LINE; FIBROBLASTS; FLOW CYTOMETRY; GENE EXPRESSION; HUMANS; LIPOFUSCIN; MALE; OXIDATIVE STRESS; TELOMERASE; TELOMERE; TRANSFECTION;

EID: 0035884738     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(01)00664-5     Document Type: Article

References (43)

1. Cellular senescence and cancer
2. Cellular senescence: Mitotic clock or culture shock?
3. Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes
4. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
5. Extension of life-span by introduction of telomerase into normal human cells
6. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span
7. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
8. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase
9. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
10. Dynamics of telomere shortening in neutrophils and T lymphocytes during ageing and the relationship to skewed X chromosome inactivation patterns
11. Subsenescent telomere lengths in fibroblasts immortalized with limiting amounts of telomerase
12. The effect of oxygen and vitamin E on the lifespan of human diploid cells in vitro
13. Oxidative DNA damage and senescence of human diploid fibroblast cells
14. Mild hyperoxia shortens telomeres and inhibits proliferation of human fibroblasts: A model for senescence?
15. The effect of low oxygen tension on the in vitro-replicative life span of human diploid fibroblast cells and their transformed derivatives
16. Similar gene expression pattern in senescent and hyperoxic-treated fibroblasts
17. Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes
18. Low oxygen concentration extends the lifespan of cultured human diploid cells
19. Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts
20. ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the post-translational activation of p53 protein involving poly(ADP-ribose) polymerase
21. Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of G-rich single stranded DNA fragments
22. Preferential accumulation of single-stranded regions in telomeres of human fibroblasts
23. Accelerated telomere shortening in fibroblasts after extended periods of confluency
24. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon
25. Stress, DNA damage and ageing - An integrative approach
26. Ribozyme cleavage of telomerase mRNA sensitizes breast tumor cells to inhibitors of topoisomerase
27. Protein carbonyl measurement by a sensitive ELISA method
28. Proteasome-dependent degradation of oxidized proteins in MRC-5 fibroblasts
29. Protein oxidation and degradation during cellular senescence in human BJ fibroblasts: Part I - Effects of proliferative senescence
30. Protein oxidation and degradation during proliferative senescence of human MRC-5 fibroblasts
31. Lipofuscin accumulation in proliferating fibroblasts in vitro: An indicator of oxidative stress
32. Short telomeres in patients with vascular dementia: An indicator of low antioxidative capacity and a possible prognostic factor?
33. Normal human chromosomes have long G-rich telomeric overhangs at one end
34. Telomere shortening is proportional to the size of the 3′-G-rich telomeric overhang
35. Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity
36. Age-dependent telomere shortening is slowed down by enrichment of intracellular vitamin C via suppression of oxidative stress
37. Asp-Ala-His-Lys (DAHK) inhibits copper-induced oxidative DNA double strand breaks and telomere shortening
38. Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening
39. The length of telomeric G-rich 3′-overhang measured by oligonucleotide ligation assay
40. Telomere length dynamics in telomerase-positive immortal human cell populations
41. Telomere dynamics in a human cancer cell line
42. Control of human telomere length by TRF1 and TRF2
43. Telomere states and cell fates