Ribozyme cleavage of telomerase mRNA sensitizes breast epithelial cells to inhibitors of topoisomerase

Cancer Research

Volumn 61, Issue 7, 2001, Pages 3053-3061

  Ludwig, A ^a   Saretzki, G ^a   Holm, P S ^a   Tiemann, F ^a   Lorenz, M ^a   Emrich, T ^a   Harley, C B ^a   Von Zglinicki, T ^a  

^a NEWCASTLE GENERAL HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CISPLATIN; CYTOTOXIC AGENT; DNA TOPOISOMERASE INHIBITOR; DOXORUBICIN; MESSENGER RNA; RIBOZYME; TELOMERASE;

APOPTOSIS; ARTICLE; BREAST EPITHELIUM; CELL CLONE; CELL IMMORTALIZATION; CELL STRAIN MCF 7; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME INHIBITION; EPITHELIUM CELL; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ADENOCARCINOMA; ANTIBIOTICS, ANTINEOPLASTIC; APOPTOSIS; BREAST; BREAST NEOPLASMS; CELL DIVISION; CELL LINE, TRANSFORMED; DNA TOPOISOMERASES, TYPE I; DNA-BINDING PROTEINS; DOXORUBICIN; EPITHELIAL CELLS; HUMANS; RNA; RNA, CATALYTIC; RNA, MESSENGER; SUBSTRATE SPECIFICITY; TELOMERASE; TUMOR CELLS, CULTURED;

EID: 0035300424     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (57)

1. Extension of life-span by introduction of telomerase into normal human cells
2. Telomeres shorten during aging of human fibroblasts
3. The RNA component of human telomerase
4. Inhibition of telomerase increases the susceptibility of human malignant glioblastoma cells to cisplatin-induced apoptosis
5. Targeted therapy of human malignant glioma in a mouse model by 2-5A antisense directed against telomerase RNA
6. Inhibition of human telomerase by 2′-O-methyl-RNA
7. Telomerase inhibition by peptide nucleic acids reverses "immortality" of transformed human cells
8. Inhibition of human telomerase in immortal human cells lead to progressive telomere shortening and cell death
9. Inhibition of telomerase limits the growth of human cancer cells
10. Telomere shortening and apoptosis in telomerase-inhibited human tumor cells
11. Hammerhead ribozyme-mediated inhibition of telomerase activity in extracts of human hepatocellular carcinoma cells
12. Synthetic 2′-O-methyl-modified hammerhead ribozymes targeted to the RNA component of telomerase as sequence-specific inhibitors of telomerase activity
13. Attenuation of telomerase activity by a hammerhead ribozyme targeting the template region of telomerase RNA in endometrial carcinoma cells
14. Preferential accumulation of single-stranded regions in telomeres of human fibroblasts
15. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: A model for senescence?
16. ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the post-translational activation of p53 protein involving poly(ADP-ribose) polymerase
17. Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts
18. Anti-apoptotic role of telomerase in pheochromocytoma cells
19. Telomerase activity and telomere length in human ovarian cancer and melanoma cell lines: Correlation with sensitivity to DNA damaging agents
20. Telomerase catalytic subunit homologs from fission yeast and human
21. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
22. Selection of a high activity ribozyme against cytostatic drug resistance-associated glypican-3 using an in vitro assay containing total tumor RNA
23. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3
24. Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli
25. Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation method
26. Intravenous administration of recombinant adenoviruses causes thrombocytopenia, anemia, and erythroblastosis in rabbits
27. A simple and efficient method for purification of infectious recombinant adenovirus
28. Neoplastic reversion accomplished by high efficiency adenoviral-mediated delivery of an anti-ras ribozyme
29. Accelerated telomere shortening in fibroblasts after extended periods of confluency
30. Apoptosis detection: An overview
31. Telomerase activity and telomere lengths in various cell lines: Changes of telomerase activity can be another method for chemosensitivity evaluation
32. Inhibition of telomerase activity and cell proliferation by a reverse transcriptase inhibitor in gynaecological cancer cell lines
33. Anthracycline-derived chemotherapeutics in apoptosis and free radical cytotoxicity
34. Monofunctional platinum amine complexes destabilize DNA significantly
35. Telomerase inhibition by induced expression of antisense RNA
36. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
37. Reactions of oxyl radicals with DNA
38. Clinical applications of anticancer drugs targeted to topoisomerase II
39. Functionally interacting telomerase RNAs in the yeast telomerase complex
40. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening
41. The telomere and telomerase: How do they interact?
42. Mammalian telomeres end in a large duplex loop
43. Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of G-rich single stranded DNA fragments
44. Cell cycle dependent localization of the telomeric PARP, tankyrase, to nuclear pore complexes and centrosomes
45. Functions of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability
46. DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes
47. Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres
48. The Saccharomyces cerevisiae Sgs1 helicase efficiently unwinds G-G paired DNAs
49. Telomerase prevents the accelerated cell aging of Werner syndrome fibroblasts
50. WRN or telomerase constructs reverse 4-nitroquinoline 1-oxide sensitivity in transformed Werner syndrome fibroblasts
51. Nuclear structure in normal and Bloom syndrome cells
52. When helicase topoisomerase meet!
53. The Bloom's syndrome gene product interacts with topoisomerase III
54. Interaction between yeast Sgs1 helicase and DNA topoisomerase III
55. Werner's syndrome lymphoblastoid cells are hypersensitive to topoisomerase II inhibitors in the G2 phase of the cell cycle
56. DNA topoisomerase II cleavage of telomeres in vitro and in vivo
57. Up-regulation of telomerase activity in human pancreatic cancer cells after exposure to etoposide