The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay

Nucleic Acids Research

Volumn 29, Issue 7, 2020, Pages

  Cimino Reale, Graziella ^a,b   Pascale, Esterina ^c   Battiloro, Eva ^a   Starace, Giuseppe ^a   Verna, Roberto ^b   D'Ambrosio, Ettore ^a  

^a CNR   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c UNIVERSITY OF L'AQUILA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; OLIGONUCLEOTIDE; SINGLE STRANDED DNA; GUANINE; LIGASE;

ARTICLE; ASSAY; CELLS; CONTROLLED STUDY; DNA STRAND; HUMAN; HUMAN CELL; OLIGONUCLEOTIDE LIGATION ASSAY; PRIORITY JOURNAL; TELOMERE LENGTH; TELOMERE SHORTENING; TELOMERIC REPEAT AMPLIFICATION PROTOCOL; CELL LINE; CELL STRAIN U937; CHEMISTRY; GENETICS; HELA CELL; METABOLISM; NUCLEOTIDE REPEAT; SOUTHERN BLOTTING; TELOMERE;

BLOTTING, SOUTHERN; CELL LINE; DNA; DNA, SINGLE-STRANDED; GUANINE; HELA CELLS; HUMANS; LIGASES; OLIGONUCLEOTIDES; REPETITIVE SEQUENCES, NUCLEIC ACID; TELOMERE; U937 CELLS;

MLCS; MLOWN;

EID: 0035315819     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/29.7.e35     Document Type: Article

References (38)

1. Muller, H.J. (1938) The remaking of chromosomes. The Collecting Net, Woods Hope (USA), 13, 181-195.
2. Mc Clintock, B. (1939) The behavior in successive nuclear divisions of a chromosome broken at meiosis. Proc. Natl Acad. Sci. USA, 25, 405-416.
3. Blackburn, E.H. (1991) Structure and function of telomeres. Nature, 350, 569-573.
4. Blackburn, E.H. (1994) Telomeres: no end in sight. Cell, 77, 621-623.
5. Zakian, V.A. (1995) Telomeres: beginning to understand the end. Science, 270, 1601-1607.
6. Greider, C.W. (1996) Telomere length regulation. Annu. Rev. Biochem., 65, 337-365.
7. Greider, C.W. and Blackburn, E.H. (1989) A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature, 337, 331-337.
8. Morin, G.B. (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell, 59, 521-529.
9. Counter, C.M., Avilion, A.A., Le Feuvre, C.E., Stewart, N.G., Greider, C.W., Harley, C.B. and Bacchetti, S. (1992) Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J., 11, 1921-1929.
10. Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V. and Cech, T.R. (1997) Reverse transcriptase motifs in the catalytic subunit of telomerase. Science, 276, 561-567.
11. Wang, J., Xie, L.Y., Allan, S., Beach, D. and Hannon, G.J. (1998) Myc activates telomerase. Genes Dev., 12, 1769-1774.
12. Wu, K.J., Grandori, C., Amacker, M., Simon-Vermot, N., Polack, A., Lingner, J. and Dalla-Favera, R. (1999) Direct activation of TERT transcription by c-MYC. Nature Genet., 21, 220-224.
13. Falchetti, M.L., Falcone, G., D'Ambrosio, E., Verna, R., Alema, S. and Levi, A. (1999) Induction of telomerase activity in v-myc-transformed avian cells. Oncogene, 18, 1515-1519.
14. Counter, C.M., Meyerson, M., Eaton, E.N., Ellisen, L.W., Caddle, S.D., Haber, D.A. and Weinberg, R.A. (1998) Telomerase activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase. Oncogene, 16, 1217-1222.
15. Bodnar, A.G., Ouellette, M., Frolkis, M., Holt, S.E., Chiu, C.P., Morin, G.B., Shay, J.W., Lichtsteiner, S. andWright, W.E. (1998) Extension of life-span by introduction of telomerase into normal human cells. Science, 279, 349-352.
16. Hahn, W.C., Counter, C.M., Lundberg, A.S., Beijersbergen, R.L., Brooks, M.W. andWeinberg, R.A. (1999) Creation of human tumour cells with defined genetic elements. Nature, 400, 464-468.
17. Herbert, B., Pitts, A.E., Baker, S.I., Hamilton, S.E., Wright, W.E., Shay, J.W. and Corey, D.R. (1999) Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proc. Natl Acad. Sci. USA, 96, 14276-14281.
18. Watson, J.D. (1972) Origin of concatameric T7 DNA. Nat. New Biol., 239, 197-201.
19. Henderson, E.R. and Blackburn, E.H. (1989) An overhanging 3' terminus is a conserved feature of telomeres. Mol. Cell. Biol., 9, 345-348.
20. Klobutcher, L.A., Swanton, M.T., Donini, P. and Prescott, D.M. (1981) All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and or unusual 3' terminus. Proc. Natl Acad. Sci. USA, 78, 3015-3019.
21. Wellinger, R.J., Wolfe, A.J. and Zakian, V.A. (1993) Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase. Cell, 72, 51-60.
22. Makarov, V., Hirose, Y. and Langmore, J.P. (1997) Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell, 88, 657-666.
23. Mc Elligott, R. and Wellinger, R.J. (1997) The terminal DNA structure of mammalian chromosomes. EMBO J., 16, 3705-3714.
24. Wright, W.E., Tesmer, V.M., Huffman, K.E., Levene, S.D. and Shay, J.W. (1997) Normal human chromosomes have long G-rich telomeric overhangs at one end. Genes Dev., 11, 2801-2809.
25. Henderson, E.R., Hardin, C.C., Walk, S.K., Tinoco, I., Jr and Blackburn, E.H. (1987) Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pair. Cell, 51, 899-908.
26. Sundquist, W.I. and Klug, A. (1989) Telomeric DNA dimerizes by formation of guanine tetrads between hairpin loops. Nature, 342, 825-829.
27. Sen, D. and Gilbert, W. (1990) A sodium-potassium switch in the formation of four-stranded G4-DNA. Nature, 344, 410-414.
28. Van Steensel, B., Smogorzewska, A. and de Lange, T. (1998) TRF2 protects human telomeres from end-to-end fusions. Cell, 92, 401-413.
29. Griffith, J.D., Comeau, L., Rosenfield, S., Stansel, R.M., Bianchi, A., Moss, H. and de Lange, T. (1999) Mammalian telomeres end in a large duplex loop. Cell, 97, 503-514.
30. Karleseder, J., Broccoli, D., Dai, Y., Hardy, S. and de Lage, T. (1999) p53 and ATM dependent apoptosis induced by telomeres lacking TRF2. Science, 283, 1321-1325.
31. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Labelling of synthetic oligonucleotides by phosphorylation with bacteriophage T4 polynucleotide kinase. Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 11.31-11.32.
32. Kim, N.W., Piatyszek, M.A., Prowse, K.R., Harley, C.B., West, M.D., Ho, P.L., Coviello, G.M., Wright, W.E., Weinrich, S.L. and Shay, J.W. (1994) Specific association of human telomerase activity with immortal cells and cancer. Science, 266, 2011-2015.
33. Tautz, D. and Renz, M. (1984) Simple sequences are ubiquitous components of eukaryotic genomes. Nucleic Acids Res., 12, 4127-4138.
34. Lundblad, V. and Szostak, J.W. (1989) A mutant with a defect in telomere elongation leads to senescence in yeast. Cell, 57, 633-643.
35. Zakian, V.A. (1996) Structure, function and replication of Saccharomyces cerevisiae telomeres. Annu. Rev. Genet., 30, 141-172.
36. Lee, H.W., Blasco, M.A., Gottlieb, G.J., Horner, J.W., II, Greider, C.W. and De Pinho, R.A. (1998) Essential role of mouse telomerase in highly proliferative organs. Nature, 392, 569-574.
37. Blasco, M.A., Gasser, S.M. and Lingner, J. (1999) Telomeres and telomerase. Genes Dev., 13, 2353-2359.
38. Huffman, K.E., Levene, S.D., Tesmer, V.M., Shay, J.W. and Wright, W.E. (2000) Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. J. Biol. Chem., 275, 19719-19722.