Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells

Nucleic Acids Research

Volumn 29, Issue 8, 2001, Pages 1653-1660

  Wang, Huichen ^a   Zeng, Zhao Chong ^a   Perrault, Ange R ^a   Cheng, Xinbo ^a   Qin, Wei ^a   Iliakis, George ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; DOUBLE STRANDED DNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PROTEIN KINASE; RESTRICTION ENDONUCLEASE; WORTMANNIN;

ARTICLE; BIOCHEMISTRY; CELL KILLING; CELL LINE; CLASSIFICATION; CONTROLLED STUDY; DNA STRAND BREAKAGE; ENZYME ACTIVITY; ENZYME DEFICIENCY; ENZYME SUBUNIT; EPISTASIS; ERROR; EXTRACT; FIBROBLAST; GENE MUTATION; GENETICS; GENOME; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; KINETICS; MAMMAL CELL; MUTANT; PLASMID; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RADIOSENSITIVITY; SENSITIZATION; VERTEBRATE;

NON-PROGRAMMATIC;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; CELL EXTRACTS; CELL SURVIVAL; CELLS, CULTURED; DNA; DNA DAMAGE; DNA LIGASES; DNA REPAIR; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; ELECTROPHORESIS, GEL, PULSED-FIELD; FIBROBLASTS; HUMANS; KINETICS; LEUKEMIA, LYMPHOCYTIC, ACUTE; MAGNESIUM; MUTATION; NUCLEAR PROTEINS; PLASMIDS; PROTEIN-SERINE-THREONINE KINASES; RADIATION TOLERANCE; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID; TUMOR CELLS, CULTURED; X-RAYS;

MAMMALIA; VERTEBRATA;

EID: 0035870583     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.8.1653     Document Type: Article

References (66)

1. DNA-dependent protein kinase stimulates an independently active, nonhomologous, end-joining apparatus
2. A surfeit of RAD51-like genes?
3. The contribution of homologous recombination in preserving genome integrity in mammalian cells
4. DNA breakage and repair
5. Efficient rejoining of radiation-induced DNA double-strand breaks in vertebrate cells deficient in genes of the RAD52 epistasis group
6. Interaction of ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids
7. The DNA-dependent protein kinase
8. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line
9. Scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair
10. Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line
11. A link between double-strand break-related repair and V(D)J recombination: The scid mutation
12. Characterization of the DNA double strand break repair defect in scid mice
13. An ionizing radiation-sensitive mutant of CHO cells: Irs-20. III. Chromosome aberrations, DNA breaks and mitotic delay
14. The scid defect results in much slower repair of DNA double-strand breaks but not high levels of residual breaks
15. Cell cycle-dependent repair of double-strand DNA breaks in a γ-ray-sensitive chinese hamster cell
16. Impairment of V(D)J recombination in double-strand break repair mutants
17. Defective repair of DNA double-strand breaks and chromosome damage in fibroblasts from a radiosensitive leukemia patient
18. Induction and rejoining of DNA double-strand breaks and interphase chromosome breaks after exposure to X rays in one normal and two hypersensitive human fibroblast cell lines
19. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination
20. A DNA double-strand break defective fibroblast cell line (180BR) derived from a radiosensitive patient represents a new mutant phenotype
21. DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes
22. Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient
23. Absence of DNA ligase IV protein in XR 1 cells: Evidence for stabilization by XRCC4
24. DNA ligase IV and XRCC4 form a stable mixed tetramer that functions synergistically with other repair factors in a cell-free end-joining system
25. Identification of Saccharomyces cerevisiae DNA ligase IV: Involvement in DNA double-strand break repair
26. Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV
27. Yeast DNA ligase IV mediates non-homologous DNA end joining
28. Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells
29. Requirement for an interaction of XRCC4 with DNA ligase IV for wild-type V(D)J recombination and DNA double-strand break repair in vivo
30. DNA ligase IV binds to XRCC4 via a motif located between rather than within its BRCT domains
31. Ku recruits the XRCC4-ligase IV complex to DNA ends
32. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase
33. An instance of clinical radiation morbidity and cellular radiosensitivity, not associated with ataxia-telagiectasia
34. The radiosensitive cell line 180BR is not defective in the major DNA damage-sensing proteins
35. Wortmannin sensitizes mammalian cells to radiation by inhibiting the DNA-dependent protein kinase-mediated rejoining of double-strand breaks
36. Detection of DNA double strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis
37. A rapid preparation of extracts for DNA replication in vitro
38. Wortmannin, a potent and selective inhibitor of phosphatidylinositol-3-kinase
39. DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product
40. Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction
41. Wortmannin inhibits repair of DNA double-strand breaks in irradiated normal human cells
42. Nonhomologous DNA end joining in cell-free systems
43. DNA ligase IV from HeLa cell nuclei
44. Mammalian DNA ligases
45. DNA end-joining catalyzed by human cell-free extracts
46. Repair of x-ray-induced DNA double-strand breaks in specific NotI restriction fragments in human fibroblasts: Joining of correct and incorrect ends
47. The nonhomologous end-joining pathway of DNA repair is required for genomic stability and the suppression of translocations
48. The nonhomologous DNA end joining pathway is important for chromosome stability in primarily fibroblasts
49. Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells
50. Radiosensitivity and oxidative signalling in ataxia telangiectasia: An update
51. ATM: A mediator of multiple responses to genotoxic stress
52. The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase
53. Substrate specificities and identification of putative substrates of ATM kinase family members
54. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination
55. Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity
56. The catalytic subunit of DNA-dependent protein kinase selectively regulates p53-dependent apoptosis but not cell-cycle arrest
57. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination
58. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates
59. Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo
60. Ku70: A candidate tumor suppressor gene for murine T cell lymphoma
61. Targeted disruption of the gene encoding DNA ligase IV leads to lethality in embryonic mice
62. Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV
63. Interplay of p53 and DNA-repair protein XRCC4 in tumorigenesis, genomic stability and development
64. DNA repair protein Ku80 suppresses chromosomal aberrations and malignant transformation
65. DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway