Active site residues of human β-glucuronidase. Evidence for GLU540 as the nucleophile and GLU451 as the acid-base residue

Journal of Biological Chemistry

Volumn 274, Issue 33, 1999, Pages 23451-23455

  Islam, M Rafiq ^a,c   Tomatsu, Shunji ^c   Shah, Gul N ^c   Grubb, Jeffrey H ^c   Jain, Sanjeev ^b,c   Sly, William S ^c  

^a NORTHWEST MISSOURI STATE UNIVERSITY   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c SAINT LOUIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; BETA GLUCURONIDASE; GLYCOSAMINOGLYCAN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

ANIMALS; BASE SEQUENCE; BINDING SITES; COS CELLS; DNA PRIMERS; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; GLUCURONIDASE; GLUTAMIC ACID; HEAT; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; SODIUM AZIDE;

ANIMALIA; ESCHERICHIA COLI; FELIS CATUS; UNIDENTIFIED BACULOVIRUS;

EID: 0033551773     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.33.23451     Document Type: Article

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