Electron cryomicroscopy methods

Current Opinion in Structural Biology

Volumn 11, Issue 5, 2001, Pages 548-554

  Unger, Vinzenz M ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AQUAPORIN; MEMBRANE PROTEIN; NUCLEAR PROTEIN; RIBONUCLEOPROTEIN;

CRYSTALLOGRAPHY; ELECTRON MICROSCOPY; HERPES VIRUS; MACROMOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; REVIEW; SPLICEOSOME; STRUCTURE ANALYSIS; TECHNIQUE; TOMOGRAPHY; VIRUS CAPSID;

HERPES; HERPESVIRIDAE; MIRIDAE;

EID: 0035442391     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(00)00260-8     Document Type: Review

References (55)

1. The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules
2. Electron crystallography: Present excitement, a nod to the past, anticipating the future
3. CCD detectors in high-resolution biological electron microscopy
4. Spot-scan imaging in transmission electron microscopy
5. Automatic focus correction for spot-scan imaging of tilted specimens
6. A maximum-likelihood approach to single-particle image refinement
7. Fast and accurate three-dimensional reconstruction from projections with random orientations via radon transforms
8. Strul - A method for 3D alignment of single-particle projections based on common line correlation in Fourier space
9. A new generation of the IMAGIC image processing system
10. EMAN: Semiautomated software for high-resolution single-particle reconstructions
11. Perforated support foils with pre-defined hole size, shape and arrangement
12. Improving the positional accuracy of the goniometer on the Philips CM series TEM
13. Leginon: An automated system for acquisition of images from vitreous ice specimens
14. Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis
15. Two-dimensional crystallogenesis of transmembrane proteins
16. Projection structure of a CIC-type chloride channel at 6.5Å resolution
17. The projection structure of EmrE, a proton-linked multidrug transporter from Escherichia coli, at 7Å resolution
18. Projection structure and oligomeric properties of a bacterial core protein translocase
19. Cryo-electron crystallography of two sub-complexes of bovine complex I reveals the relationship between the membrane and peripheral arms
20. Projection structure of a plant vacuole membrane aquaporin by electron cry-crystallography
21. Three-dimensional structure of the ion-coupled transport protein NhaA
22. The three-dimensional map of microsomal glutathione transferase 1 at 6Å resolution
23. The projection structure of the membrane protein microsomal glutathione transferase at 3Å resolution as determined from two-dimensional hexagonal crystals
24. Projection structure of NhaA, a secondary transporter from Escherichia coli, at 4.0Å resolution
25. Structural determinants of water permeation through aquaporin-1
26. Visualization of a water-selective pore by electron crystallography in vitreous ice
27. Structure of the alpha beta tubulin dimer by electron crystallography
28. Protein crystallization on lipid layers and structure determination of the RNA polymerase II transcription initiation complex
29. Structure analysis of soluble proteins using electron crystallography
30. Structure of membrane-bound annexin A5 trimers: A hybrid cryo-EM - X-ray crystallography study
31. A new look at the microtubule binding patterns of dimeric kinesins
32. Evidence for cleft closure in actomyosin upon ADP release
33. Structure of the utrophin actin-binding domain bound to F-actin reveals binding by an induced fit mechanism
34. Nicotinic acetylcholine receptor at 4.6Å resolution: Transverse tunnels in the channel wall
35. Locating the thapsigargin-binding site on Ca(2+)-ATPase by cryoelectron microscopy
36. 2+-ATPase in E1 and E2 states
37. Cryo-electron microscopy structure of an SH3 amyloid fibril and model of the molecular packing
38. Recent developments in cryo-electron microscopy reconstruction of single particles
39. Molecular machines: Putting the pieces together
40. Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle
41. Structure of α-latrotoxin oligomers reveals that divalent cation-dependent tetramers form membrane pores
42. Seeing the herpesvirus capsid at 8.5Å
43. Bridging the information gap: Computational tools for intermediate resolution structure interpretation
44. Quantitative fitting of atomic models into observed densities derived by electron microscopy
45. Situs: A package for docking crystal structures into low-resolution maps from electron microscopy
46. High-resolution model of the microtubule
47. Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
48. Structure of the bacteriorhodopsin mutant F219L N intermediate revealed by electron crystallography
49. Maturation dynamics of a viral capsid: Visualization of transitional intermediate states
50. Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle
51. The ribosome - A macromolecular machine par excellence
52. The architecture of active zone material at the frog's neuromuscular junction
53. Cryo-electron tomography of Neurospora mitochondria
54. Toward detecting and identifying macromolecules in a cellular context: Template matching applied to electron tomograms
55. Fraternal twins: AQP1 and GlpF