Molecular mechanism of vectorial proton translocation by bacteriorhodopsin

Nature

Volumn 406, Issue 6796, 2000, Pages 653-657

  Subramanlam, Sriram ^a,b   Henderson, Richard ^b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIORHODOPSIN; PROTON PUMP; RETINAL; SCHIFF BASE;

ARTICLE; BACTERIAL MEMBRANE; CONFORMATIONAL TRANSITION; COVALENT BOND; CRYSTALLOGRAPHY; HALOBACTERIUM SALINARUM; ISOMERISM; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTON TRANSPORT;

BACTERIORHODOPSINS; BIOLOGICAL TRANSPORT; CRYSTALLOGRAPHY, X-RAY; CYTOPLASM; PROTEIN CONFORMATION; PROTONS; RETINALDEHYDE; SCHIFF BASES;

EID: 0034632864     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35020614     Document Type: Article

References (28)

1. Henderson, R. et al. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213, 899-929 (1990).
2. Subramaniam, S. The structure of bacteriorhodopsin: an emerging consensus. Curr. Opin. Struct. Biol. 9, 462-468 (1999).
3. Subramaniam, S. et al. Protein conformational changes in the bacteriorhodopsin photocycle. J. Mol. Biol. 287, 145-161 (1999).
4. Vonck, J. Structure of the bacteriorhodopsin mutant F2191. N intermediate revealed by electron crystallography. EMBO J. 19, 2152-2160 (2000).
5. Luecke, H., Schobert, B., Richter, H. -T., Cartailler, J. -P., & Lanyi, J. K. Structural changes in bacteriorhodopsin during ion transport at 2 Å resolution. Science 286, 255-260 (1999).
6. Sass, H.-J. et al. Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin. Nature 406, 649-653 (2000).
7. Dencher, N. A., Dresselhaus, D., Zaccai, G. & Buldt, G. Structural changes in bacteriorhodopsin during proton translocation revealed by neutron diffraction. Proc. Natl Acad. Sci. USA 86, 7876-7879 (1989).
8. Koch, M. H. J. et al. Time-resolved X-ray diffraction study of structural changes associated with the photocycle of bacteriorhodopsin. EMBO J. 10, 521-526 (1991).
9. Kamikubo, H. et al. The last phase of the reprotonation switch in bacteriorhodopsin. The transition between the M-type and the N-type protein conformation depends on hydration. Biochemistry 36, 12282-12287 (1996).
10. Subramaniam, S., Gerstein, M., Oesterhelt, D. & Henderson, R. Electron diffraction analysis of structural changes in the photocyole of bacteriorhodopsin. EMBO J. 12, 1-8 (1993).
11. Oka, T. et al. Conformational change of helix G in the bacteriorhodopsin photocycle: Investigation with heavy atom labeling and X-ray diffraction. Biophys. J. 76, 1018-1023 (1999).
12. Fodor, S. P. et al. Chromophore structure in bacteriorhodopsin's N intermediate: implications for the proton pumping mechanism. Biochemistry 27, 7097-7101 (1988).
13. Elia, G. R., Childs, R. F., Britten, J. E. Yang, D. S. C. & Santarsiero, B. D. Structure and wavelength modification in retinylidene iminium salts. Can. J. Chem. 74, 591-601 (1996).
14. Santarsiero, B. D., James, M. N. G., Mahendran, M. & Childs, R. F. The crystal structure of N-methyl-N-phenyl-retinylideneiminium perchlorate: a structural model for the bacteriorhodopsin chromophore. J. Am. Chem. Soc. 112, 9416-9418 (1990).
15. Hamanaka, T., Mitsui, T., Ashida, T. & Kakudo, M. The crystal structure of all-trans retinal. Acta Crystallogr. B 28, 214-222 (1972).
16. 2. Acta Crystallogr. B 28, 2936-2945 (1972).
17. Simmons, C. J., Liu, R. S. H., Denny, M. & Seff, K. The crystal structure of 13-cis-retinal. The molecular structures of its 6-s-cis and 6-s-trans conformers. Acta Crystallogr. B 37, 2197-2205 (1981).
18. 2). Acta Crystallogr. C 42, 711-715 (1986).
19. Moltke, S. et al. The angles between the C-1-, C-5-, and C-9-methyl bonds of the retinylidene chromophore and the membrane normal increase in the M-intermediate of bacteriorhodopsin: Direct determination with solid-state H-2 NMR. Biochemistry 38, 11762-11772 (1999).
20. Griffiths, J. M. et al. Structural investigation of the active site in bacteriorhodopsin: Geometric constraints on the roles of Asp-85 and Asp-212 in the proton pumping mechanism from solid-state NMR. Biochemistry 39, 362-371 (2000).
21. Brown, I., S. et al. A local electrostatic change is the cause of the large scale protein conformation shift in bacteriorhodopsin. Proc. Natl Acad. Sci. USA 94, 5040-5044 (1997).
22. Schobert, B. & Lanyi, J. K. Halorhodopsin is a light-driven chloride pump. J. Biol. Chem. 257, 306-313 (1982).
23. Hoff, W. D., Jung, K. H. and Spudich, J. L. Molecular mechanism of photosignaling by archaeal sensory rhodopsins. Ann. Rev. Biophys. Biomol. Struct. 26, 223-258 (1997).
24. Altenbach, C. et al. Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study. Biochemistry 35, 12470-12478 (1996).
25. Faruqi, A. R., Henderson, R. & Subramaniam, S. Cooled CCD detector with tapered fibre optics for recording electron diffraction patterns. Ultramicroscopy 75, 235-250 (1999).
26. Grigorieff, N., Ceska, T. A., Downing, K. H., Baldwin, J. M. & Henderson, R. Electron-crystallographic refinement of the structure of bacteriorhodopsin. J. Mol. Biol. 259, 393-421 (1996).
27. Brunger, A. T. et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905-921 (1998).
28. Essen, L. O., Siegert, R., Lehmannn, W. D. & Oesterhelt, D. Lipid patches in membrane protein oligomers: Crystal structure of the bacteriorhodopsin-lipid complex. Proc. Natl Acad. Sci. USA 95, 11673-1167 (1998).