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note
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-1. The second image was taken at about 2 μm farther away from the defocus of the first image. The second image, which was not included in the final reconstruction, was used to provide the orientation estimation for the corresponding particles in the first image. The micrographs were digitized with a Zeiss SCAI microdensitometer (Carl Zeiss, Englewood, CO) with a 7-μm step size (corresponding to 1.4 Å per pixel on the specimen).
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0015242164
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Determination of the center and orientation parameters and their subsequent projection-based refinement were carried out on a 24-processor SGI Onyx 2 supercomputer with procedures described previously (28), which are based on Fourier common lines [R. A. Crowther, Philos. Trans. R. Soc. London Ser. B 261, 221 (1971); S. D. Fuller Cell 48, 923 (1987)].
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Crowther, R.A.1
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0023666171
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Determination of the center and orientation parameters and their subsequent projection-based refinement were carried out on a 24-processor SGI Onyx 2 supercomputer with procedures described previously (28), which are based on Fourier common lines [R. A. Crowther, Philos. Trans. R. Soc. London Ser. B 261, 221 (1971); S. D. Fuller Cell 48, 923 (1987)].
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note
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Supported by NIH, National Center for Research Resources, NSF, and the Human Frontier Science Program. Z.H.Z. is a Pew Scholar in Biomedical Sciences and a Basil O'Connor Starter Scholar of the March of Dimes Birth Defects Foundation. We thank M. F. Schmid for discussions.
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