A new look at the microtubule binding patterns of dimeric kinesins

Journal of Molecular Biology

Volumn 297, Issue 5, 2000, Pages 1087-1103

  Hoenger, Andreas ^a   Thormählen, Manfred ^b   Diaz Avalos, Ruben ^c   Doerhoefer, Monika ^a   Goldie, Kenneth N ^a   Müller, Jens ^b   Mandelkow, Eckhard ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b MAX PLANCK UNIT FOR STRUCTURAL MOLECULAR BIOLOGY   (Germany)

^c FLORIDA STATE UNIVERSITY   (United States)

Author keywords

Cryo electron microscopy; Helical 3D image reconstructions; Kinesin; Kinesin movement; Microtubules

Indexed keywords

KINESIN;

ARTICLE; CRYOELECTRON MICROSCOPY; IMAGE RECONSTRUCTION; MICROTUBULE ASSEMBLY; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; STOICHIOMETRY; STRUCTURE ANALYSIS;

EID: 0034646568     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3627     Document Type: Article

References (67)

1. Proteolytic mapping of kinesin/Ncd-microtubule interface: Nucleotide-dependent conformational-changes in the loops L8 and L12
2. Nucleotide-dependent conformations of the kinesin dimer interacting with microtubules
3. Three-dimensional structure of functional motor proteins on microtubules
4. Leading the procession: New insights into kinesin motors
5. Cruising along microtubule highways: How membranes move through the secretory pathway
6. The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain
7. Determination of microtubule polarity by cryoelectron microscopy
8. Kinesin takes one 8-nm step for each ATP that it hydrolyzes
9. On the hand-over-hand footsteps of kinesin heads
10. Walking talking heads
11. Cryo-electron microscopy of vitrified biological specimens
12. Determinants of molecular motor directionality
13. Determinants of kinesin polarity
14. Pathway of processive ATP hydrolysis by kinesin
15. Alternating site mechanism of the kinesin ATPase
16. Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis
17. Kinesin's processivity results from a mechanical and chemical coordination between the ATP hydrolysis cycles of the two motor domains
18. Reversal of the direction of movement of a molecular motor
19. Kinesin and dynein superfamily proteins and the mechanism of organelle transport
20. Nucleotide dependent angular change in kinesin motor domain bound to tubulin
21. Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules
22. Nucleotide-dependent structural changes in dimeric Ncd Molecules complexed to microtubules
23. Congruent docking of dimeric kinesin and ncd into three-dimensional electron cryomicroscopy maps of microtubule-motor ADP complexes
24. Polarity of 2D and 3D maps of tubulin sheets and motor decorated sheets
25. Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry
26. Three-dimensional structure of a tubulin-motor-protein complex
27. Image reconstruction of microtubles decorated with monomeric and dimeric kinesins: Comparison with X-ray structure and implications for motility
28. The movement of kinesin along microtubules
29. Influence of the kinesin neck domain on dimerization and ATPase kinetics
30. Three-dimensional structure of the kinesin head-microtubule complex
31. 15 Å resolution model of the monomeric kinesin motor KIF1A
32. The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate
33. The crystal structure of dimeric kinesin and implications for microtubule-dependent motility
34. Model of the microtubule-kinesin complex based on electron cryo-microscopy and X-ray crystallography
35. Molscript: A program to produce both detailed and schematic plots of protein structures
36. Crystal structure of the kinesin motor domain reveals a structural similarity to myosin
37. Structures of kinesin and kinesin-microtubule interaction
38. The structural and mechanochemical cycle of kinesin
39. Unstrained microtubles studied by cryo-electron microscopy: Substructure, supertwist and disassembly
40. Tubulin hoops
41. Conformations of kinesin: Solution versus crystal structures and interactions with microtubules
42. Identification of kinesin neck region as a stable alpha-helical coiled-coil and its thermodynamic characterization
43. Pathway of ATP hydrolysis by monomeric and dimeric kinesin
44. Factors influencing the precision of quantitative scanning transmission electron microscopy
45. The structure of the nucleotide-binding site of kinesin
46. Structure of the αβ-tubulin dimer by electron crystallography
47. High-resolution model of the microtubule
48. A structural change in the kinesin motor protein that drives motility
49. Role of the kinesin neck region in processive microtubule-based motility
50. Crystal structure of the motor domain of the kinesin-related motor ncd
51. Direction determination in the minus-end-directed kinesin motor Ncd
52. X-ray structure of motor and neck domains from rat brain kinesin
53. Reduced surface: An efficient way to compute molecular surfaces
54. SUPRIM: Easily modified image processing software
55. Motor and cargo interactions
56. Three-dimensional structure of ncd-decorated microtubules obtained by a back-projection method
57. A model for the microtubule-Ncd motor protein complex obtained by cryo-electron microscopy and image analysis
58. Three different approaches for calculating the 3-dimensional structure of microtubules decorated with kinesin motor domains
59. Use of T7 RNA polymerase to direct expression of cloned genes
60. Direct observation of kinesin stepping by optical trapping interferometry
61. Interaction of monomeric and dimeric kinesin with microtubules
62. The coiled-coil helix in the neck of kinesin
63. Demonstration of coiled-coil interactions within the kinesin neck region using synthetic peptides - Implications for motor activity
64. Single kinesin molecules studied with a molecular force clamp
65. Characterization of microtubule protofilament numbers; how does the surface lattice accommodate?
66. PHOELIX: A package for semi-automated helical reconstruction
67. Microtubule interaction site of the kinesin motor