Spliceosomal UsnRNP biogenesis, structure and function

Current Opinion in Cell Biology

Volumn 13, Issue 3, 2001, Pages 290-301

  Will, C L ^a   Luhrmann R ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG; URIDINE; URIDINE RICH SMALL NUCLEAR RIBONUCLEOPROTEIN;

BIOGENESIS; CATALYSIS; CRYSTAL STRUCTURE; HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; REVIEW; RNA SPLICING; SPLICEOSOME; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING;

EID: 0035370526     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0955-0674(00)00211-8     Document Type: Review

References (88)

1. Splicing of precursors to mRNA by the spliceosome
2. snRNP structure and function
3. Splicing of precursors to mRNAs by the spliceosomes
4. Monomethylated cap structures facilitate RNA export from the nucleus
5. A cap-binding protein complex mediating U snRNA export
6. CRM1 is an export receptor for leucine-rich nuclear export signals
7. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus
8. PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation
9. The snRNP core assembly pathway: Identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro
10. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins
11. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis
12. SMN mutants of spinal muscular atrophy patients are defective in binding to snRNP proteins
13. Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli
14. Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN)
15. SMN tudor domain structure and its interaction with the Sm proteins
16. The C-terminal RG dipeptide repeats of the spliceosomal Sm proteins D1 and D3 contain symmetrical dimethylarginines, which form a major B-cell epitope for anti-Sm autoantibodies
17. Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations
18. U snRNP assembly in yeast involves the La protein
19. A role for the yeast La protein in U6 snRNP assembly: Evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts
20. Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding
21. U1 snRNA is cleaved by RNase III and processed through an Sm site-dependent pathway
22. Nucleocytoplasmic transport and processing of small nuclear RNA precursors
23. Alternative 3′-end processing of U5 snRNA by RNase III
24. Functions of the exosome in rRNA, snoRNA and snRNA synthesis
25. Yeast exosome mutants accumulate 3′-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs
26. Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast
27. Nucleo-cytoplasmic transport of U snRNPs: Definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap
28. Nuclear import of U snRNPs requires importin beta
29. Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure
30. Functional characterization of nuclear localization signals in yeast Sm proteins
31. Posttranscriptional modifications in the U small nuclear RNAs
32. Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing
33. A small nucleolar guide RNA functions both in 2′O-ribose methylation and pseudouridylation of the U5 spliceosomal RNA
34. Function and synthesis of small nucleolar RNAs
35. Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA
36. An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B′ spliceosome proteins
37. Modification of U6 spliceosomal RNA is guided by other small RNAs
38. Nucleolar factors direct the 2′-O-ribose methylation and pseudouridylation of U6 spliceosomal RNA
39. Transient nucleolar localization of U6 small nuclear RNA in Xenopus laevis oocytes
40. A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
41. Characterization of U6 snRNA-protein interactions
42. Characterization of Sm-like proteins in yeast and their association with U6 snRNA
43. Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin
44. A Sm-like protein complex that participates in mRNA degradation
45. Yeast Sm-like proteins function in mRNA decapping and decay
46. snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions
47. Sm and Sm-like proteins belong to a large family: Identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs
48. Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs
49. The spliceosomal snRNP core complex of Trypanosoma brucei: Cloning and functional analysis reveals seven Sm protein constituents
50. The Sm domain is an ancient RNA binding motif with oligo[U] specificity
51. Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure
52. Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
53. Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle
54. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs
55. The 20kD protein of human [U4/U6.U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60 kD and 90 kD proteins
56. Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin
57. Functional interaction of a novel 15.5 kD [U4/U6.U5] tri-snRNP protein with the 5′ stem-loop of U4 snRNA
58. Crystal structure of the spliceosomal 15.5 kDa protein bound to a U4 snRNA fragment
59. A common core RNP structure shared between the small nucleolar box C/D RNPs and the spliceosomal U4 snRNP
60. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis
61. Identification of both shared and distinct proteins in the major and minor spliceosomes
62. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
63. Spliceosome assembly
64. Protein functions in pre-mRNA splicing
65. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins
66. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
67. Luc7p, a novel yeast U1 snRNP protein with a role in 5′ splice site recognition
68. Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection
69. A yeast intronic splicing enhancer and Nam8p are required for Mer1p-activated splicing
70. The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing
71. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
72. Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs
73. Antibodies specific for N6-methyladenosine react with intact snRNPs U2 and U4/U6
74. Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions
75. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
76. The second catalytic step of pre-mRNA splicing
77. Functional recognition of 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
78. The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
79. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
80. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
81. Active disruption of an RNA-protein interaction by a DExH/D RNA helicase
82. Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
83. Dynamic exchanges of RNA interactions leading to catalytic core formation in the U12-dependent spliceosome
84. RNA-RNA interactions in nuclear pre-mRNA splicing
85. Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome
86. Metal ion catalysis during splicing of premessenger RNA
87. Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center
88. Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome