Functional association of U2 snRNP with the ATP-independent splicecosomal complex E

Molecular Cell

Volumn 5, Issue 5, 2000, Pages 779-787

  Das, Rita ^a   Zhou, Zhaolan ^a   Reed, Robin ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ADENOVIRUS; ARTICLE; MOLECULAR BIOLOGY; NONHUMAN; PLASMID; PROTEIN ASSEMBLY; PROTEIN BINDING; SPLICEOSOME;

ADENOVIRIDAE;

EID: 0033636518     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)80318-4     Document Type: Article

References (59)

1. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
2. Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA
3. Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes
4. Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions
5. Protein components specifically associated with prespliceosome and spliceosome complexes
6. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
7. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition
8. Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9
9. Separation of splicing factor SF3 into two components and purification of S3a activity
10. Splicing of precursors to mRNAs by the spliceosomes
11. The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein
12. Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch site mutations
13. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly
14. Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals
15. Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels
16. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155
17. U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction
18. Initial recognition of U12-dependent introns requires both U11/5' splice-site and U12/branch-point interactions
19. A novel set of spliceosome-associated proteins and the essential splicing factor PSG bind stably to pre-mRNA prior to catalytic step II of the splicing reaction
20. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A
21. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site
22. Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleo-protein particles in the spliceosome
23. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns
24. Association of U2 snRNP with the spliceosomal complex E
25. An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract
26. The spliceosome assembly pathway in mammalian extracts
27. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
28. U11 snRNA interacts in vivo with the 5' splice site of U12-dependent (AU-AC) pre-mRNA introns
29. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs
30. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes
31. Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex
32. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP
33. Requirements for U2 snRNP addition to yeast pre-mRNA
34. Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs
35. Inhibition of msl-2 splicing by sex lethal reveals functional interaction between U2AF35 and the 3' splice site AG
36. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
37. A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals
38. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA
39. Three recognition events at the branch-site adenine
40. A minimal spliceosomal complex A recognizes the branch site and polypyrimidine tract
41. Mechanisms of fidelity during pre-mRNA splicing
42. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly
43. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
44. SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
45. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly
46. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
47. An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p
48. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
49. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro
50. Pre-mRNA splicing: The discovery of a new spliceosome doubles the challenge
51. AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes
52. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
53. Functional recognition of the 3' splice site AG by the splicing factor U2AF35
54. Cloning and domain structure of the mammalian splicing factor U2AF
55. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
56. Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing
57. Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles
58. Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans
59. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing