Dynamic exchanges of RNA interactions leading to catalytic core formation in the U12-dependent spliceosome

Molecular Cell

Volumn 7, Issue 1, 2001, Pages 217-226

  Frilander, Mikko J ^a   Steitz, Joan A ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2' O METHYL RNA OLIGONUCLEOTIDE; OLIGONUCLEOTIDE; RNA HELICASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CATALYSIS; EXON; PROTEIN CROSS LINKING; RNA SPLICING; SPLICEOSOME;

EID: 0035104772     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00169-1     Document Type: Article

References (54)

1. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
2. A U1/U4/U5 snRNP complex induced by a 2′-O-methyl-oligoribonucleotide complementary to U5 snRNA
3. A novel U1/U5 interaction indicates proximity between U1 and U5 snRNAs during an early step of mRNA splicing
4. Evolutionary fates and origins of U12-type introns
5. Splicing of precursors to mRNAs by the spliceosome
6. A two-step mechanism for 5′ and 3′ splice-site pairing
7. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo
8. Cis-acting elements distinct from the 5′ splice site promote U1-independent pre-mRNA splicing
9. Functionally redundant interactions between U2 and U6 spliceosomal snRNAs
10. Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions
11. Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice-sites
12. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns
13. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
14. Base pairing with U6atac snRNA is required for 5′ splice site activation of U12-dependent introns in vivo
15. Involvement of U6 snRNA in 5′ splice site selection
16. U4/U5/U6 snRNP recognizes the 5′ splice site in the absence of U2 snRNP
17. Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly
18. Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation
19. Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome
20. The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
21. Mutations in U6 snRNA that alter splice site specificity: Implications for the active site
22. A spontaneous duplication in U6 spliceosomal RNA uncouples the early and late functions of the ACAGA element in vivo
23. Purification of telomerase from Euplotes aediculatus: Requirement of a primer 3′ overhang
24. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes
25. A role for U2/U6 helix Ib in 5′ splice site selection
26. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome
27. Functional recognition of the 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
28. Site-specific modification of pre-mRNA: The 2′-hydroxyl groups at the splice sites
29. Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage
30. U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites
31. snRNA interactions at 5′ and 3′ splice sites monitored by photoactivated crosslinking in yeast spliceosomes
32. RNA-RNA interactions in nuclear pre-mRNA splicing
33. The pre-mRNA 5′ cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5′ splice sites
34. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
35. A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
36. The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5′ splice site
37. Evidence for a base-pairing interaction between U6 small nuclear RNA and the 5′ splice site during the splicing reaction in yeast
38. The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA
39. Conservation of functional features of U6atac and U12 snRNAs between vertebrates and higher plants
40. The U5 and U6 small nuclear RNAs as active site components of the spliceosome
41. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
42. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
43. A novel spliceosome containing U11, U12 and U5 snRNPs excises a minor class (AT-AC) intron in vitro
44. Highly divergent U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
45. Pre-mRNA splicing: The discovery of a new spliceosome doubles the challenge
46. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
47. The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p
48. Interaction of small nuclear RNA's with precursor messenger RNA during in vitro splicing
49. Identification of both shared and distinct proteins in the major and minor spliceosomes
50. Purine-rich enhancers function in the AT-AC pre-mRNA splicing pathway and do so independently of intact U1 snRNP
51. Site-specific crosslinking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing
52. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing
53. 4SU) into specific positions in a long RNA molecule
54. Site-specific crosslinking of mammalian U11 and U6atac to the 5′ splice site of an AT-AC intron