Metal ion catalysis during splicing of premessenger rna

Nature

Volumn 388, Issue 6644, 1997, Pages 801-805

  Sontheimer, Erik J ^a   Sun, Sengen ^a   Piccirilli, Joseph A ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; METAL ION;

ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA SEQUENCE; RNA SPLICING; SPLICEOSOME; TETRAHYMENA;

ADENOVIRIDAE; CATALYSIS; CHLORIDES; HELA CELLS; HUMANS; MAGNESIUM CHLORIDE; MANGANESE COMPOUNDS; METALS; RNA PRECURSORS; RNA SPLICING; SULFUR;

EUKARYOTA; TETRAHYMENA;

EID: 0030833215     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/42068     Document Type: Article

References (28)

1. Krämer, A. in Pre-mRNA Processing (ed. Lamond, A.) 35-64 (Landes, Austin, 1995).
2. Steitz,T. A. & Steitz, J. A. A general two-metal-ion mechanism for catalytic RNA. Proc. Natl Acad. Sci. USA 90, 6498-6502 (1993).
3. Piccirilli, J. A., Vyle, J. S., Caruthers, M. H. & Cech, T. R. Metal ion catalysis in the Tetrahymena ribozyme reaction. Nature 361, 85-88 (1993).
4. Cosstick, R. & Vyle, J. S. Synthesis and properties of dithymidine phosphate analogues containing 3′thiothymidine. Nucleic Acids Res, 18, 829-835 (1990).
5. Jaffe, E. K. & Cohn, M. Divalent cation-dependent stereospecificity of adenosine 5′-O(2-thiotriphosphate) in the hexokinase and pyruvate kinase reactions: The absolute stereochemistry of the diastereomers of adenosine 5′-O-(2-thiotriphosphate). J. Biol. Chem. 253, 4823-4825 (1978).
6. Jaffe, E. K. & Cohn, M. Diastereomers of the nucleoside phosphorothioates as probes of the structure of the metal nucleotide substrates and of the nucleotide binding site of yeast hexokinase. J. Biol. Chem. 254, 10839-10845 (1979).
7. 2+ complexes of adenine nucleotides and thionucleotides and rate constants for formation and dissociation of MgATP and MgADP. Biochemistry 23, 5262-5271 (1984).
8. 2-) in aqueous solution. J. Am. Chem. Soc. 119, 744-755 (1997).
9. Moore, M. J. & Sharp, P. A. Site-specific modification of pre-mRNA: The 2′-hydroxyl groups at the splice sites. Science 256, 992-997 (1992).
10. Solnick, D. Trans splicing of mRNA precursors. Cell 42, 157-164 (1985).
11. Pearson, R. G. Acids and bases. Science 151, 172-177 (1966).
12. Dantzmann, C. L. & Kiessling, L. L. Reactivity of a 2′-thio nucleotide analog. J. Am. Chem. Soc. 118, 11715-11719 (1996).
13. Burgin, A. B., Huizenga, B. N. & Nash, H. A. A novel suicide substrate for DNA topoisomerases and site-specific recombinases. Nucleic Acids Res. 23, 2973-2979 (1995).
14. Seiwert, S. D. & Steitz, J. A. Uncoupling two functions of the U1 small nuclear ribonucleoprotein particle during in vitro splicing. Mol. Cell. Biol. 13, 3135-3145 (1993).
15. Vyle, J. S., Connolly, B. A., Kemp, D. & Cosstick, R. Sequence- and strand-specific cleavage in oligodeoxyribonucleotides and DNA containing 3′-thiothymidine. Biochemsitry 31, 3012-3018 (1992).
16. Weinstein, L. B., Earnshaw, D. J., Cosstick, R. & Cech, T. R. Synthesis and characterization of an RNA dinucleotide containing a 3′-S-phosphorothiolate linkage. J. Am. Chem. Soc. 118, 10341-10350 (1996).
17. Konarska, M. M. & Sharp, P. A. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell 46, 845-855 (1986).
18. Reyes, J. L., Kois, P., Konforti, B. B. & Konarska, M. M. The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome. RNA 2, 213-225 (1996).
19. Narlikar, G. J., Gopalakrishnan, V., McConnell, T. S., Usman, N. & Herschlag, D. Use of binding energy by an RNA enzyme for catalysis by positioning and substrate destabilization. Proc. Natl Acad. Sci. USA 92, 3668-3672 (1995).
20. Aebi, M., Hornig, H., Padgett, R. A., Reiser, J. & Weissmann, C. Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA. Cell 47, 555-565 (1986).
21. Tarn, W.-Y. Site-specific substitution of inosine at the terminal positions of a pre-mRNA intron: Implications for the configuration of the terminal base interaction. Biochimie (in the press).
22. Anderegg, G. in Comprehensive Coordination Chemistry: The Synthesis, Reactions, Properties and Applications of Coordination Compounds (eds Wilkinson, G., Gillard, R. D. & McCleverty, J. A.) 777-792 (Pergamon, Oxford, 1987).
23. Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson, M. R. & Steitz, T. A. Cocrystal structure of an editing complex of Klenow fragment with DNA. Proc. Natl Acad. Sci. USA 85, 8924-8928 (1988).
24. Moore, M. J. & Sharp, P. A. Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature 365, 364-368 (1993).
25. Sontheimer, E. J. & Steitz, J. A. The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science 262, 1989-1996 (1993).
26. Query, C. C., Moore, M. J. & Sharp, P. A. Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model. Genes Dev. 8, 587-597 (1994).
27. Dignam, J. D., Lebovitz, R. M. & Roeder, R. G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489 (1983).
28. Abmayr, S. M., Reed, R. & Maniatis, T. Identification of a functional mammalian spliceosome containing unspliced pre-mRNA. Proc. Natl Acad. Sci. USA 85, 7216-7220 (1988).