Functional recognition of the 5' splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly

Molecular Cell

Volumn 6, Issue 2, 2000, Pages 317-328

  Maroney, Patricia A ^a   Romfo, Charles M ^a   Nilsen, Timothy W ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; BINDING SITE; CIS TRANS ISOMERISM; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; NEMATODE; NONHUMAN; PROTEIN ASSEMBLY; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; RIBONUCLEASE PROTECTION ASSAY; RNA SEQUENCE; RNA SPLICING; SITE DIRECTED MUTAGENESIS; SPLICEOSOME; TRANS SPLICING;

EID: 0033634673     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)00032-0     Document Type: Article

References (66)

1. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
2. A U1/U4/U5 snRNP complex induced by a 2'-O-methyl-oligonucleotide complementary to U5 snRNA
3. A novel U1/U5 interaction indicates proximity between U1 and U5 snRNas during an early step of mRNA splicing
4. Finding splice sites within a wilderness of RNA
5. Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein
6. U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6
7. Trans-splicing and polycistronic transcription in Caenorhabditis elegans
8. Splicing of precursors to mRNAs by the spliceosomes
9. Breast the clock: Paradigms for NTPases in the maintenance of biological fidelity
10. The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein
11. A two-step mechanism for 5' and 3' splice-site pairing
12. Suppression of mammalian 5' splice-site defects by U1 small nuclear RNAs from a distance
13. Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene
14. Conversion of a trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site
15. Mutations in the conserved loop of human U5 snRNA generate the use of novel cryptic 5' splice sites in vivo
16. A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing
17. Cis-acting elements distinct from the 5' splice site promote U1-independent pre-mRNA splicing
18. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
19. 3'-terminal labeling of RNA with T4 RNA ligase
20. Pathways for selection of 5' splice sites by U1 snRNPs adn SF2/ASF
21. The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes
22. Trans-splicing of nematode pre-messenger RNA in vitro
23. U small nuclear ribonucleoprotein requirements for nematode cis- and trans-splicing in vitro
24. Mechanisms for selecting 5' splice sites in mammalian pre-mRNA splicing
25. U1 snRNA promotes the selection of nearby 5' splice sites by U6 snRNA in mammalian cells
26. U4/U5/U6 snRNP recognizes the 5' splice site in the absence of U2 snRNP
27. A short 5' splice site RNA oligo can participate in both steps of splicing in mammalian extracts
28. Disruption of base pairing between the 5' splice site and the 5' end of U1 snRNA is required for spliceosome assembly
29. Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome
30. Rapid and efficient site-specific mutagenesis without phenotypic selection
31. Antisense probes containing 2-Amino-adenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts
32. RNase H cleavage for processing of in vitro transcribed RNA for NMR studies and RNA ligation
33. The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases
34. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
35. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes
36. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
37. Site-specific modification of pre-mRNA: The 2' hydroxyl groups at the splice sites
38. Splicing of precursors to mRNAs by the spliceosome
39. The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro
40. The role of U5 snRNP in pre-mRNA splicing
41. Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage
42. U5 snRNA interacts with exon sequences at 5' and 3' splice sites
43. snRNA interactions at 5' and 3' splice sites monitored by photoactivated cross-linking in yeast spliceosomes
44. RNA-RNA interactions in the spliceosome: Unraveling the ties that bind
45. Trans-splicing
46. RNA-RNA interactions in nuclear pre-mRNA splicing
47. The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast
48. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
49. Spliceosome assembly
50. The canonical GU dinucleotide at the 5' splice site is recognized by p220 of the U5 snRNP within the spliceosome
51. Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing
52. Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts
53. 3' splice site recognition in S. cerevisiae does not require base pairing with U1 snRNA
54. The spliceosomal U small nuclear RNAs of Ascaris lumbricoides
55. The U5 and U6 small nuclear RNAs as active site components of the spliceosome
56. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
57. An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p
58. SR proteins can compensate for the loss of U1 snRNP function in vitro
59. Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps
60. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
61. snRNP Structure and function
62. 35
63. Site-specific crosslinking of mammalian U5 snRNP to the 5' splice site prior to the first step of premessenger RNA splicing
64. Functional reconstitution of U6 snRNA in nematode cis and trans-splicing: U6 can serve as both a branch acceptor and a 5' exon
65. A new method for detecting sites of 2'-O-methylation in RNA molecules
66. 35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing