Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center

Genes and Development

Volumn 13, Issue 15, 1999, Pages 1983-1993

  Siatecka, Miroslawa ^a   Reyes, José L ^a,b   Konarska, Maria M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

5' splice site; Catalytic center; Prp8; Spliceosome; US snRNP protein

Indexed keywords

SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA;

ARTICLE; GENE MUTATION; GENE REPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN RNA BINDING; RNA SPLICING; SPLICEOSOME;

EID: 0033179750     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.13.15.1983     Document Type: Article

References (54)

1. Abovich, N. and M. Rosbash. 1997. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell 89: 403-412.
2. Achsel, T., K. Ahrens, H. Brahms, S. Teigelkamp, and R. Lührmann. 1998. The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol. Cell Biol. 18: 6756-6766.
3. Anderson, G.J., M. Bach, R. Lührmann, and J.D. Beggs. 1989. Conservation between yeast and man of a protein associated with U5 snRNP. Nature 342: 819-821.
4. Becker, D.M. and L. Guarente. 1991. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194: 182-187.
5. Burge, C.B., T.H. Tuschl, and P.A. Sharp. 1998. Splicing of precursors to mRNAs by the spliceosomes. In RNA world II (ed. R.F. Gesteland, T.R. Cech, and J.F. Atkins), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Burley, S.K. and R.G. Roeder. 1996. Biochemistry and structural biology of transcription factor IID (TFIID). Annu. Rev. Biochem. 65: 769-799.
7. Cadwell, R.C. and G.F. Joyce. 1992. Randomization of genes by PCR mutagenesis. PCR Methods Appl. 2: 28-33.
8. Chiara, M.D., L. Paladjian, R.F. Kramer, and R. Reed. 1997. Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals. EMBO J. 16: 4746-4759.
9. Collins, C. and C. Guthrie. 1999. Prp8 constrains a tertiary interaction at the spliceosomal active site. Genes & Dev. (this issue).
10. Dietrich, R.C., R. Incorvaia, and R.A. Padgett. 1997. Terminal intron dinucleotide sequences do not distinguish between U2-and U12-dependent introns. Mol. Cell 1: 151-160.
11. Dix, I., C.S. Russell, R.T. O'Keefe, A.J. Newman, and J.D. Beggs. 1998. Protein-RNA interactions in the U5 snRNP of S. cerevisiae. RNA 4: 1239-1250.
12. Fouser, L.A. and J.D. Friesen. 1986. Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing. Cell 45: 81-93.
13. Frank, D. and C. Guthrie. 1992. An essential splicing factor, SLU7, mediates 3′ splice site choice in yeast. Genes & Dev. 6: 2112-2124.
14. Guthrie, C. and G.R. Fink. 1991. Guide to yeast genetics and molecular biology, (ed. J.N. Abelson and M.I. Simon), Vol. 194. Academic Press, San Diego, CA.
15. Hodges, P.E., S.P. Jackson, J.D. Brown, and J.D. Beggs. 1995. Extraordinary sequence conservation of the PRP8 splicing factor. Yeast 11: 337-342.
16. Jacquier, A., J.R. Rodriguez, and M. Rosbash. 1985. A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing. Cell 43: 423-430.
17. Kandels-Lewis, S. and B. Séraphin. 1993. Role of U6 snRNA in 5′ splice site selection. Science 262: 2035-2039.
18. Kao, H.-Y. and P.G. Siliciano. 1996. Identification of Prp40, a novel essential yeast splicing factor associated with the U1 snRNP. Mol. Cell. Biol. 16: 960-967.
19. Karin, M., R. Najarian, A. Haslinger, P. Valenzuela, and J. Welch. 1984. Primary structure and transcription of an amplified locus: The CUP1 locus of yeast. Proc. Natl. Acad. Sci. 81: 337-341.
20. Kim, C.H. and J. Abelson. 1996. Site-specific crosslinks of yeast U6 snRNA to the pre-mRNA near the 5′ splice site. RNA 2: 995-1010.
21. Konforti, B.B. and M.M. Konarska. 1994. U4/U5/U6 snRNP recognizes the 5′ splice site in the absence of U2 snRNP. Genes & Dev. 8: 1962-1973.
22. Konforti, B.B., M.J. Koziolkiewicz, and M.M. Konarska. 1993. Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly. Cell 75: 863-873.
23. Krämer, A. 1996. The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem. 65: 367-409.
24. Kuhn, A.N., Z. Li, and D.A. Brow. 1999. Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome. Mol. Cell 3: 65-75.
25. Kunkel, T.A., J.D. Roberts, and R.A. Zakour. 1987. Rapid and efficient site directed mutagenesis without phenotypic selection. In Methods in enzymology, recombinant DNA (ed. R. Wu and L. Grossman), pp. 367-382. Academic Press, San Diego, CA.
26. Lesser, C.F. and C. Guthrie. 1993a. Mutations in U6 snRNA that alter splice site specificity: Implications for the active site. Science 262: 1982-1988.
27. _. 1993b. Mutational analysis of pre-mRNA splicing in S. cerevisiae using a sensitive new reporter gene, CUP1. Genetics 133: 851-863.
28. Luo, H.R., G.A. Moreau, N. Levin, and M.J. Moore. 1999. The human Prp8 protein is a component of both U2-and U12-dependent spliceosomes. RNA (in press).
29. Luukkonen, B.G.M. and B. Seraphin. 1998a. Genetic interaction between U6 snRNA and the first intron nucleotide in S. cerevisiae. RNA 4: 167-180.
30. _. 1998b. A role for U2/U6 helix Ib in 5′ splice site selection. RNA 4: 915-927.
31. MacMillan, A.M., C.C. Query, C.R. Allerson, S. Chen, G.L. Verdine, and P.A. Sharp. 1994. Dynamic association of proteins with the pre-mRNA branch region. Genes & Dev. 8: 3008-3020.
32. Moore, M.J. and P.A. Sharp. 1993. Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature 365: 364-368.
33. Moore, M.J., C.C. Query, and P.A. Sharp. 1993. Splicing of precursors to mRNA by the spliceosome. In The RNA world (ed. R.F. Gesteland and J.F. Atkins), pp. 303-357. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. Newman, A.J. and C. Norman. 1992. U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites. Cell 68: 743-754.
35. Nilsen, T.W. 1998. RNA-RNA interactions in nuclear pre-mRNA splicing. In RNA structure and function (ed. R. Simons and M. Grunberg-Manago), pp. 279-307. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
36. Pikielny, C.W., B.C. Rymond, and M. Rosbash. 1986. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes. Nature 324: 341-345.
37. Reyes, J.L., P. Kois, B.B. Konforti, and M.M. Konarska. 1996. The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome. RNA 2: 213-225.
38. Reyes, J.L., E.H. Gustafson, H.R. Luo, M.J. Moore, and M.M. Konarska. 1999. The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5′ splice site. RNA 5: 167-179.
39. Rosbash, M. and B. Séraphin. 1991. Who's on first? The U1 snRNP-5′ splice site interaction and splicing. Trends Biochem. Sci. 16: 187-190.
40. Ruskin, B. and M.R. Green. 1985. Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing. Nature 317: 732-734.
41. Sha, M., T. Levy, P. Kois, and M.M. Konarska. 1998. Probing of the spliceosome with site-specifically derivatized 5′ splice site RNA oligonucleotides. RNA 4: 1069-1082.
42. Sharp, P.A. and C.B. Burge. 1997. Classification of introns: U2-type or U12-type. Cell 91: 875-879.
43. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 3-20.
44. Sontheimer, E.J. and J.A. Steitz. 1993. The U5 and U6 snRNAs as active site components of the spliceosome. Science 262: 1989-1996.
45. Sontheimer, E.J., S. Sun, and J.A. Piccirilli. 1997. Metal ion catalysis during splicing of pre-mRNA. Nature 388: 801-805.
46. Staley, J.P. and C. Guthrie. 1999. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p. Mol. Cell 3: 55-64.
47. Strauss, E.J. and C. Guthrie. 1991. A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes & Dev. 5: 629-641.
48. Tarn, W.-Y. and J.A. Steitz. 1996. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro. Cell 84: 801-811.
49. Teigelkamp, S., A.J. Newman, and J.D. Beggs. 1995. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA. EMBO J. 14: 2602-2612.
50. Umen, J.G. and C. Guthrie. 1995. A novel role for a U5 snRNP protein in 3′ splice site selection. Genes & Dev. 9: 855-868.
51. _. 1996. Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection. Genetics 143: 723-739.
52. Vijayraghavan, U., R. Parker, J. Tamm, Y. Iimura, J. Rossi, J. Abelson, and C. Guthrie. 1986. Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J. 5: 1683-1695.
53. Will, C.L. and R. Lührmann. 1997. Protein functions in pre-mRNA splicing. Curr. Opin. Cell Biol. 9: 320-328.
54. Wyatt, J.R., E.J. Sontheimer, and J.A. Steitz. 1992. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing. Genes Dev. 6: 2542-2553.