Chemical amplification: Continuous-flow PCR on a chip

Science

Volumn 280, Issue 5366, 1998, Pages 1046-1048

  Kopp, Martin U ^a   De Mello, Andrew J ^a   Manz, Andreas ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; DNA; DNA POLYMERASE; DNA TOPOISOMERASE (ATP HYDROLYSING); DOUBLE STRANDED DNA;

AMPLIFIER; ARTICLE; BASE PAIRING; COOLING; DEVICE; FLUORESCENCE; GENE; HEATING; LIGASE CHAIN REACTION; NEISSERIA GONORRHOEAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TEMPERATURE;

DNA TOPOISOMERASES, TYPE II; GENES, BACTERIAL; GLASS; NEISSERIA GONORRHOEAE; POLYMERASE CHAIN REACTION; TEMPERATURE; TEMPLATES, GENETIC; TIME FACTORS;

NEISSERIA GONORRHOEAE; PROKARYOTA;

EID: 0032524099     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5366.1046     Document Type: Article

References (12)

1. K. B. Mullis, F. Ferré, R. A. Gibbs, The Polymerase Chain Reaction (Birkhäuser, Boston, 1994).
2. H. Swerdlow, K. Dew-Jager, R. F. Gesteland, Biotechniques 15, 512 (1993).
3. J. Cheng, M. A. Shoffner, K. R. Mitchelson, L. J. Kricka, P. Wilding, J. Chromatogr. A 732, 151 (1996).
4. A. T. Woolley et al., Anal. Chem. 68, 4081 (1996).
5. A. Manz and H. Becker, Eds., Microsystem Technology in Chemistry and Life Sciences, vol. 194 of Topics in Current Chemistry (Springer, Berlin, 1998).
6. E. L. Cussler, Diffusion Mass Transfer in Fluid Systems (Cambridge Univ. Press, Cambridge, 1984).
7. N. Chiem and D. J. Harrison, Anal. Chem. 69, 373 (1997).
8. U. D. Larsen, J. Branebjerg, G. Blankenstein, in Proceedings of the 2nd International Symposium on Miniaturized Total Analysis Systems μTAS '96 (Analytical Methods & Instrumentation, Base 1, 1996), pp. 228-230.
9. T. Laurell and J. Drott, Biosens. Bioelectronics 10, 289 (1995).
10. D. E. Raymond, A. Manz, H. M. Widmer, Anal. Chem. 66, 2858 (1994).
11. C. S. Effenhauser, A. Paulus, A. Manz, H. M. Widmer, ibid., p. 2949.
12. Supported by SmithKline Beecham, Zeneca, and a grant from the Biotechnology and Biological Sciences Research Council (UK). We thank B. Robertson, for primers and templates used in this study, and A. Ivens. We also thank Hybaid for the loan of their PCR instrument and the Alberta Microelectronic Centre for the production of the microchips.