Protein structural dynamics by single-molecule fluorescence polarization

Progress in Biophysics and Molecular Biology

Volumn 74, Issue 1-2, 2000, Pages 1-35

  Forkey, Joseph N ^a   Quinlan, Margot E ^a   Goldman, Yale E ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PROTEIN; RHODAMINE;

BIOPHYSICS; FLUORESCENCE POLARIZATION; INSTRUMENTATION; MATHEMATICAL MODEL; MODEL; MOTION; NUCLEAR MAGNETIC RESONANCE; OPTICS; PROTEIN STRUCTURE; REVIEW; SCANNING ELECTRON MICROSCOPY; SPECTROSCOPY; X RAY CRYSTALLOGRAPHY;

BIOPHYSICS; FLUORESCENCE POLARIZATION; MODELS, THEORETICAL; MOTION; PROTEINS;

EID: 0034536115     PISSN: 00796107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0079-6107(00)00015-8     Document Type: Review

References (114)

1. Single molecule detection and photochemistry on a surface using near-field optical excitation
2. Single-molecule detection with total internal reflection excitation: Comparing signal-to-background and total signals in different geometries
3. Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal
4. Total internal reflection fluorescence microscopy
5. Fluorescence polarization microscopy
6. Total internal reflection fluorescence
7. Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts
8. Single molecules observed by near-field scanning optical microscopy
9. Near-field optics: Microscopy, spectroscopy, and surface modification beyond the diffraction limit
10. Fluorescence and photobleaching dynamics of single light-harvesting complexes
11. The dynamics of structural deformations of immobilized single light-harvesting complexes
12. The ATP synthase - A splendid molecular machine
13. Absorption and emission of evanescent photons
14. Fluorescence polarization: Measurement with ultraviolet-polarizing filters in a spectrophotofluorometer
15. Structural information for explaining the molecular mechanism of protein biosynthesis
16. Measurement of ATPase activites of myosin at the level of tracks and single molecules
17. Dynamic measurement of myosin light-chain-domain tilt and twist in muscle contraction
18. A homobifunctional rhodamine for labeling proteins with defined orientations of a fluorophore
19. Understanding, improving and using green fluorescent proteins
20. Some aspects of excited-state probe emission spectroscopy for structure and dynamics of model and biological membranes
21. Photon counting techniques with silicon avalanche photodiodes
22. On/off blinking and switching behaviour of single molecules of green fluorescent protein
23. Simultaneous imaging of individual molecules aligned both parallel and perpendicular to the optic axis
24. Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels
25. Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: Visualization of the pre-power stroke state
26. + conduction and selectivity
27. Near-field optical-scanning microscopy
28. Labeling of calmodulin with a new bifunctional bodipy for improved fluorophore stability in anisotropy measurements
29. Three-dimensional orientation measurements of symmetric single chromophores using polarization microscopy
30. Quaternary changes in topoisomerase II may direct orthogonal movement of two DNA strands
31. Single-molecule structural dynamics by fluorescence polarization microscopy
32. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
33. Wag the tail: Structural dynamics of actomyosin
34. Specific covalent labeling of recombinant protein molecules inside live cells
35. Single molecule polarization spectroscopy: Pentacence in pterphenyl
36. Single molecule spectroscopy: Fluorescence excitation spectra with polarized light
37. Single molecule dynamics studied by polarization modulation
38. Hindered rotational diffusion and rotational jumps of single molecules
39. Polarization spectroscopy of single fluorescent molecules
40. Singlemolecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism
41. Single-molecule anisotropy imaging
42. Engineering green fluorescent protein for improved brightness, longer wavelengths, and fluorescence resonance energy transfer
43. Flourescence emission at dielectric and metal-film interfaces
44. Optical microscopic observation of single small molecules
45. Fluorescence polarization transients from rhodamine isomers on the myosin regulatory light chain in skeletal muscle fibers
46. Atomic structure of scallop myosin subfragment S1 complexed with MgADP: A novel conformation of the myosin head
47. Assay of microtubule movement driven by single kinesin molecules
48. Watching proteins move using site-directed spin labeling
49. Fluorescent probes for living cells
50. Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium
51. Protein rearrangements underlying slow inactivation of the Shaker K + channel
52. Single-molecule enzymatic dynamics
53. Imaging and time-resolved spectroscopy of single molecules at an interface
54. Direct physical measure of conformational rearrangement underlying potassium channel gating
55. Fluorescent analogs of peptides and hormones
56. Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics
57. Chapter 4: Fluorescence polarization studies of myosin and muscle crossbridges
58. Optical detection and spectroscopy of single molecules in a solid
59. Illuminating single molecules in condensed matter
60. Time-resolved site-directed spin-labeling studies of bacteriorhodopsin: Loop-specific conformational changes in M
61. Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method
62. Optical detection of single molecules
63. Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal
64. High-resolution spectroscopy of organic molecules in solids: From fluorescence line narrowing and hole burning to single molecule spectroscopy
65. Transient detection of spin-labeled myosin subfragment 1 conformational states during ATP hydrolysis
66. 2 relaxation by mutual cancellation of dipoledipole coupling and chemical shift anisotrophy indicates an avenue to NMR structures of very large biological macromolecules in solution
67. Imaging individual green fluorescent proteins
68. Tilting of the light chain region in single myosin molecules using total internal reflection fluorescence polarization microscopy
69. Structure of the actin-myosin complex and its implications for muscle contraction
70. Three-dimensional structure of myosin subfragment-1: A molecular motor
71. Dynamics of translation on the ribosome: Molecular mechanics of translocation
72. Single molecule rotational and translational diffusion observed by near-field scanning optical microscopy
73. Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres
74. Polarization measurements of single fluorophores attached to motor protein in aqueous solution
75. Real time imaging of single fluorophores on moving actin with an epifluorescence microscope
76. Axial rotation of sliding actin filaments revealed by singlefluorophore imaging
77. Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis
78. Characterization of photophysics and mobility of single molecules in fluid lipid membrane
79. Imaging single-molecule dichroism
80. Detection of single fluorescent molecules
81. Single-molecule detection of rhodamine 6G in ethanolic solutions using continuous wave laser excitation
82. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: Cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion
83. Peptidyl transferase center activity observed in single ribosomes
84. Order in supported phospholipid monolayers detected by the dichroism of fluorescence excited with polarized evanescent illumination
85. Time-resolved spectroscopy of single molecules using near-field and far-field optics
86. Near-field spectroscopy of single molecules at room temperature
87. The green fluorescent protein
88. Single-molecule fluorescence observed with mercury lamp illumination
89. The orientation of transition moments of dye molecules used in fluorescence studies of muscle systems
90. Excitation wavelength dependent fluorescence anisotropy of eosin-myosin adducts
91. Covalent labeling of proteins and nucleic acids with fluorophores
92. The regulation of catalysis in ATP synthase
93. High-resolution NMR of encapsulated proteins dissolved in lowviscosity fluids
94. Fluorescent analog cytochemistry: Tracing functional protein components in living cells
95. Myosin conformational states determined by single fluorophore polarization
96. Fluorescence spectroscopy of single biomolecules
97. Probing single molecule dynamics
98. Optical studies of single molecules at room temperature
99. Molecular basis of charge movement in voltage-gated sodium channels
100. Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein
101. 1-ATPase is a highly efficient molecular motor that rotates with discrete 120° steps