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Volumn 274, Issue 5289, 1996, Pages 966-969

Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels

Author keywords

[No Author keywords available]

Indexed keywords

BROWNIAN MOVEMENT; GELS; MICROSCOPIC EXAMINATION; MOLECULES; POLYAMIDES; POROUS MATERIALS; PROTEINS; THREE DIMENSIONAL;

EID: 0030575902     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5289.966     Document Type: Article
Times cited : (350)

References (34)
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    • Vale, R.D.1
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    • N. Catsimpoolas, Ed. Plenum, New York
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    • (1976) Methods of Protein Separation , vol.2 , pp. 145-218
    • Rodbard, D.1
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    • J.-R. Daban, M. Samso, S. Bartolome, Anal. Biochem. 199, 162 (1991); ibid., p. 169.
    • Anal. Biochem. , pp. 169
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    • D. L. Taylor and Y.-L. Wang, Eds. Academic Press. San Diego, CA
    • At a glass/solution boundary, this approximation holds to within 10%; the slight deviation from exponentiality arises from near-field coupling of the dipole radiation into the more optically dense glass. See, for example, D. Axelrod and E. H. Hellen, in Fluorescence Microscopy of Living Cells in Culture, Part B, D. L. Taylor and Y.-L. Wang, Eds. (Academic Press. San Diego, CA, 1989), pp. 399-416.
    • (1989) Fluorescence Microscopy of Living Cells in Culture, Part B , pp. 399-416
    • Axelrod, D.1    Hellen, E.H.2
  • 33
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    • note
    • 2〉 values (Table 1) is ≃2.7%, thus indicating that, within our experimental error, diffusion in axial and transverse directions is equivalent.
  • 34
    • 10544242609 scopus 로고    scopus 로고
    • note
    • We thank L. S. B. Goldstein and R. Sakowicz for useful discussions and for the Cy5-labeled antibody, and B. H. Zimm for a critical reading of the manuscript. D. J. N. was supported in part by a National Science Foundation Postdoctoral Fellowship.


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