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Volumn 36, Issue 3, 1999, Pages 224-231

Single-molecule detection with total internal reflection excitation: Comparing signal-to-background and total signals in different geometries

Author keywords

B phycoerythrin; Fluorescence imaging; Photobleaching; Rhodamine 6G; Single molecule detection; Total internal reflection

Indexed keywords

RHODAMINE 6G;

EID: 0033168828     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19990701)36:3<224::AID-CYTO12>3.0.CO;2-J     Document Type: Article
Times cited : (71)

References (45)
  • 1
    • 4244084400 scopus 로고
    • Optical-detection and spectroscopy of single molecules in a solid
    • Moerner WE, Kador L. Optical-detection and spectroscopy of single molecules in a solid. Phys Rev Lett 1989;62:2535-2538.
    • (1989) Phys Rev Lett , vol.62 , pp. 2535-2538
    • Moerner, W.E.1    Kador, L.2
  • 2
    • 0040391480 scopus 로고
    • Single pentacene molecules detected by fluorescence excitation in a para-terphenyl crystal
    • Orrit M, Bernard J. Single pentacene molecules detected by fluorescence excitation in a para-terphenyl crystal. Phys Rev Lett 1990;65: 2716-2719.
    • (1990) Phys Rev Lett , vol.65 , pp. 2716-2719
    • Orrit, M.1    Bernard, J.2
  • 3
    • 0025920682 scopus 로고
    • Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal
    • Ambrose WP, Moerner WE. Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal. Nature 1991;349: 225-227.
    • (1991) Nature , vol.349 , pp. 225-227
    • Ambrose, W.P.1    Moerner, W.E.2
  • 6
    • 36448999220 scopus 로고
    • Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence
    • Wilkerson CW Jr, Goodwin PM, Ambrose WP, Martin JC, Keller RA. Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence. Appl Phys Lett 1993;62: 2030-2032.
    • (1993) Appl Phys Lett , vol.62 , pp. 2030-2032
    • Wilkerson C.W., Jr.1    Goodwin, P.M.2    Ambrose, W.P.3    Martin, J.C.4    Keller, R.A.5
  • 7
    • 33748586492 scopus 로고    scopus 로고
    • Photochemistry and fluorscence emission dynamics of single molecules in solution: B-phycoerythrin
    • Wu M, Goodwin PM, Ambrose WP, Keller RA. Photochemistry and fluorscence emission dynamics of single molecules in solution: B-phycoerythrin. J Phys Chem 1996;100:17406-17409.
    • (1996) J Phys Chem , vol.100 , pp. 17406-17409
    • Wu, M.1    Goodwin, P.M.2    Ambrose, W.P.3    Keller, R.A.4
  • 9
    • 0000854652 scopus 로고    scopus 로고
    • Single-molecule detection in liquids by laser-induced fluorescence
    • Goodwin PM, Ambrose WP, Keller RA. Single-molecule detection in liquids by laser-induced fluorescence Accounts Chem Res 1996;29: 607-613.
    • (1996) Accounts Chem Res , vol.29 , pp. 607-613
    • Goodwin, P.M.1    Ambrose, W.P.2    Keller, R.A.3
  • 11
    • 0028104148 scopus 로고
    • Probing individual molecules with confocal fluorescence microscopy
    • Nie S, Chiu DT, Zare RN. Probing individual molecules with confocal fluorescence microscopy. Science 1994;266:1018-1021.
    • (1994) Science , vol.266 , pp. 1018-1021
    • Nie, S.1    Chiu, D.T.2    Zare, R.N.3
  • 13
    • 0000195179 scopus 로고
    • Digital chemical-analysis of dilute microdroplets
    • Ng KC, Whitten WB, Arnold S, Ramsey JM. Digital chemical-analysis of dilute microdroplets. Anal Chem 1992;64:2914-2919.
    • (1992) Anal Chem , vol.64 , pp. 2914-2919
    • Ng, K.C.1    Whitten, W.B.2    Arnold, S.3    Ramsey, J.M.4
  • 14
    • 0027698450 scopus 로고
    • Single molecules observed by near-field scanning optical microscopy
    • Betzig E, Chichester RJ. Single molecules observed by near-field scanning optical microscopy. Science 1993;262:1422-1425.
    • (1993) Science , vol.262 , pp. 1422-1425
    • Betzig, E.1    Chichester, R.J.2
  • 15
    • 0028762166 scopus 로고
    • Single-molecule detection and photochemistry of a surface using near-field optical-excitation
    • Ambrose WP, Goodwin PM, Martin JC, Keller RA. Single-molecule detection and photochemistry of a surface using near-field optical-excitation. Phys Rev Lett 1994;72:160-163.
    • (1994) Phys Rev Lett , vol.72 , pp. 160-163
    • Ambrose, W.P.1    Goodwin, P.M.2    Martin, J.C.3    Keller, R.A.4
  • 16
    • 0028734960 scopus 로고
    • Fluorescence detection of single molecules using near-field optical excitation and time correlated photon-counting
    • Ambrose WP, Goodwin PM, Martin JC, Keller RA. Fluorescence detection of single molecules using near-field optical excitation and time correlated photon-counting SPIE 1994;2125:2-11.
    • (1994) SPIE , vol.2125 , pp. 2-11
    • Ambrose, W.P.1    Goodwin, P.M.2    Martin, J.C.3    Keller, R.A.4
  • 17
    • 0028137086 scopus 로고
    • Alterations of single molecule fluorescence lifetimes in near-field optical microscopy
    • Ambrose WP, Goodwin PM, Martin JC, Keller RA. Alterations of single molecule fluorescence lifetimes in near-field optical microscopy. Science 1994;265:364-367.
    • (1994) Science , vol.265 , pp. 364-367
    • Ambrose, W.P.1    Goodwin, P.M.2    Martin, J.C.3    Keller, R.A.4
  • 18
    • 0028137085 scopus 로고
    • Probing single-molecule dynamics
    • Xie XS, Dunn RC. Probing single-molecule dynamics. Science 1994;265: 361-364.
    • (1994) Science , vol.265 , pp. 361-364
    • Xie, X.S.1    Dunn, R.C.2
  • 19
    • 0028269587 scopus 로고
    • Near-field spectroscopy of single molecules at room-temperature
    • Trautman JK, Macklin JJ, Brus LE, Betzig E. Near-field spectroscopy of single molecules at room-temperature. Nature 1994;369:40-42.
    • (1994) Nature , vol.369 , pp. 40-42
    • Trautman, J.K.1    Macklin, J.J.2    Brus, L.E.3    Betzig, E.4
  • 21
    • 0029350755 scopus 로고
    • Super resolution imaging and detection of fluorescence from single molecules by scanning near-field optical microscopy
    • Meixner J, Zeisel D, Bopp MA, Tarrach G. Super resolution imaging and detection of fluorescence from single molecules by scanning near-field optical microscopy. Opt Eng 1995;34:2324-2332.
    • (1995) Opt Eng , vol.34 , pp. 2324-2332
    • Meixner, J.1    Zeisel, D.2    Bopp, M.A.3    Tarrach, G.4
  • 22
    • 0029987587 scopus 로고    scopus 로고
    • Probing the interaction between 2 single molecules: Fluorescence resonance energy-transfer between a single-donor and a single-acceptor
    • Ha T, Enderle T, Ogletree DF, Chemla DS, Selvin PR, Weiss S. Probing the interaction between 2 single molecules: fluorescence resonance energy-transfer between a single-donor and a single-acceptor. Proc Natl Acad Sci USA 1996;93:6264-6268.
    • (1996) Proc Natl Acad Sci USA , vol.93 , pp. 6264-6268
    • Ha, T.1    Enderle, T.2    Ogletree, D.F.3    Chemla, D.S.4    Selvin, P.R.5    Weiss, S.6
  • 23
    • 0000793711 scopus 로고    scopus 로고
    • Imaging and time-resolved spectroscopy of single molecules at an interface
    • Macklin JJ, Trautman JK, Harris TD, Brus LE. Imaging and time-resolved spectroscopy of single molecules at an interface. Science 1996;272:255-258.
    • (1996) Science , vol.272 , pp. 255-258
    • Macklin, J.J.1    Trautman, J.K.2    Harris, T.D.3    Brus, L.E.4
  • 24
  • 26
    • 0031032395 scopus 로고    scopus 로고
    • Single-molecule spectral fluctuations at room-temperature
    • Lu HP, Xie XS. Single-molecule spectral fluctuations at room-temperature Nature 1997;385:143-146.
    • (1997) Nature , vol.385 , pp. 143-146
    • Lu, H.P.1    Xie, X.S.2
  • 27
    • 0028404256 scopus 로고
    • Single-molecule detection by laser-induced fluorescence technique with a position-sensitive photon-counting apparatus
    • Ishikawa M, Hirano K, Hayakawa T, Hosoi S, Brenner S. Single-molecule detection by laser-induced fluorescence technique with a position-sensitive photon-counting apparatus. Jpn J Appl Phys 1994; 33:1571-1576.
    • (1994) Jpn J Appl Phys , vol.33 , pp. 1571-1576
    • Ishikawa, M.1    Hirano, K.2    Hayakawa, T.3    Hosoi, S.4    Brenner, S.5
  • 29
    • 0141673857 scopus 로고
    • Optical probing of single molecules of terrylene in a Shpolskii matrix: A 2-state single molecule switch
    • Moerner WE, Plakhotnik T, Irngartinger T, Croci M, Palm V, Wild UP. Optical probing of single molecules of terrylene in a Shpolskii matrix: a 2-state single molecule switch. J Phys Chem 1994;98:7382-7389.
    • (1994) J Phys Chem , vol.98 , pp. 7382-7389
    • Moerner, W.E.1    Plakhotnik, T.2    Irngartinger, T.3    Croci, M.4    Palm, V.5    Wild, U.P.6
  • 30
    • 0029129917 scopus 로고
    • Real-time imaging of single fluorophores on moving actin with an epifluorescence microscope
    • Sase I, Miyata H, Corrie JET, Craik JS, Kinosita K. Real-time imaging of single fluorophores on moving actin with an epifluorescence microscope. Biophys J 1995;69:323-328.
    • (1995) Biophys J , vol.69 , pp. 323-328
    • Sase, I.1    Miyata, H.2    Corrie, J.E.T.3    Craik, J.S.4    Kinosita, K.5
  • 31
    • 33750626346 scopus 로고
    • Characterization of photophysics and mobility of single molecules in a fluid lipid-membrane
    • Schmidt T, Schütz GJ, Baumgartner W, Gruber HJ, Schindler H. Characterization of photophysics and mobility of single molecules in a fluid lipid-membrane. J Phys Chem 1995;99:17662-17668.
    • (1995) J Phys Chem , vol.99 , pp. 17662-17668
    • Schmidt, T.1    Schütz, G.J.2    Baumgartner, W.3    Gruber, H.J.4    Schindler, H.5
  • 33
    • 0028945654 scopus 로고
    • Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous-solution
    • Funatsu T, Harada Y, Tokunaga M, Saito K, Yanagida T. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous-solution. Nature 1995;374:555-559.
    • (1995) Nature , vol.374 , pp. 555-559
    • Funatsu, T.1    Harada, Y.2    Tokunaga, M.3    Saito, K.4    Yanagida, T.5
  • 34
    • 0030575902 scopus 로고    scopus 로고
    • 3-Dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels
    • Dickson RM, Norris DJ, Tzeng YL, Moerner WE. 3-Dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels. Science 1996;274:966-969.
    • (1996) Science , vol.274 , pp. 966-969
    • Dickson, R.M.1    Norris, D.J.2    Tzeng, Y.L.3    Moerner, W.E.4
  • 35
    • 0030832226 scopus 로고    scopus 로고
    • On/off blinking and switching behavior of single molecules of green fluorescent proteins
    • Dickson RM, Cubitt AB, Tsien RY, Moerner WE. On/off blinking and switching behavior of single molecules of green fluorescent proteins. Nature 1997;388:355-358.
    • (1997) Nature , vol.388 , pp. 355-358
    • Dickson, R.M.1    Cubitt, A.B.2    Tsien, R.Y.3    Moerner, W.E.4
  • 36
    • 0031037896 scopus 로고    scopus 로고
    • Direct measurement of single-molecule diffusion and photodecomposition in free solution
    • Xu XH, Yeung ES. Direct measurement of single-molecule diffusion and photodecomposition in free solution. Science 1997;275:1106-1109.
    • (1997) Science , vol.275 , pp. 1106-1109
    • Xu, X.H.1    Yeung, E.S.2
  • 37
    • 0039617818 scopus 로고    scopus 로고
    • Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in-vitro
    • Iwane H, Funatsu T, Harada Y, Tokunaga M, Ohara O, Morimoto S, Yanagida T. Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in-vitro. FEBS Lett 1997;407:235-238.
    • (1997) FEBS Lett , vol.407 , pp. 235-238
    • Iwane, H.1    Funatsu, T.2    Harada, Y.3    Tokunaga, M.4    Ohara, O.5    Morimoto, S.6    Yanagida, T.7
  • 38
    • 0031560940 scopus 로고    scopus 로고
    • Single-molecule imaging of fluorophores and enzymatic-reactions achieved by objective-type total internal-reflection fluorescence microscopy
    • Tokunaga M, Kitamura K, Saito K, Iwane AH, Yanagida T. Single-molecule imaging of fluorophores and enzymatic-reactions achieved by objective-type total internal-reflection fluorescence microscopy. Biochem Biophys Res Commun 1997;235:47-53.
    • (1997) Biochem Biophys Res Commun , vol.235 , pp. 47-53
    • Tokunaga, M.1    Kitamura, K.2    Saito, K.3    Iwane, A.H.4    Yanagida, T.5
  • 40
    • 0001160237 scopus 로고    scopus 로고
    • Single-molecule imaging of fluorescently labeled proteins on metal by surface-plasmon in aqueous-solution
    • Yokota H, Saito K, Yanagida T. Single-molecule imaging of fluorescently labeled proteins on metal by surface-plasmon in aqueous-solution. Phys Rev Lett 1998;80:4606-4609.
    • (1998) Phys Rev Lett , vol.80 , pp. 4606-4609
    • Yokota, H.1    Saito, K.2    Yanagida, T.3
  • 41
    • 0024490584 scopus 로고
    • Total internal-reflection fluorescence microscopy
    • Axelrod D. Total internal-reflection fluorescence microscopy. Methods Cell Biol 1989;30:245-270
    • (1989) Methods Cell Biol , vol.30 , pp. 245-270
    • Axelrod, D.1
  • 42
    • 0016236319 scopus 로고
    • Interactions of light with monomolecular dye layers
    • Wolf E, editor. Amsterdam: North-Holland
    • Drexhage KH. Interactions of light with monomolecular dye layers. In: Wolf E, editor. Progress in optics Volume XII. Amsterdam: North-Holland; 1974. p 163.
    • (1974) Progress in Optics , vol.12 , pp. 163
    • Drexhage, K.H.1
  • 43
    • 0001640805 scopus 로고
    • Light-emission by magnetic and electric dipoles close to a plane dielectric interface: 3. Radiation-patterns of dipoles with arbitrary-orientation
    • Lukosz W. Light-emission by magnetic and electric dipoles close to a plane dielectric interface: 3. Radiation-patterns of dipoles with arbitrary-orientation. J Opt Soc Am 1979;69:1495-1503.
    • (1979) J Opt Soc Am , vol.69 , pp. 1495-1503
    • Lukosz, W.1
  • 44
    • 0032128026 scopus 로고    scopus 로고
    • Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of 2-step photolysis
    • Eggeling C, Widengren J, Rigler R, Seidel CAM. Photobleaching of fluorescent dyes under conditions used for single-molecule detection: evidence of 2-step photolysis. Anal Chem 1998;70:2651-2659.
    • (1998) Anal Chem , vol.70 , pp. 2651-2659
    • Eggeling, C.1    Widengren, J.2    Rigler, R.3    Seidel, C.A.M.4
  • 45
    • 0027093323 scopus 로고
    • Solvation, crystallization, crystal-structure analysis and refinement of B-phycoerythrin from the red alga porphyridium-sordidum at 2.2 angstrom resolution
    • Ficner R, Lobeck K, Schmidt G, Huber R. Solvation, crystallization, crystal-structure analysis and refinement of B-phycoerythrin from the red alga porphyridium-sordidum at 2.2 angstrom resolution. J Mol Biol 1992;228:935-950.
    • (1992) J Mol Biol , vol.228 , pp. 935-950
    • Ficner, R.1    Lobeck, K.2    Schmidt, G.3    Huber, R.4


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